Tumor cell response to drug induced apoptosis is a function of intra cellular redox status 1

... activity of H2O2 was a function of activation of the apoptotic death pathway as evidenced by the significant increase in caspase activity, cleavage of the caspase-3 substrate PARP, and appearance of ... drug- induced intracellular H2O2 production This fits in well with our hypothesis that a balance between intracellular O2Ϫ and H2O2 could be a critical factor in the resp...

Ngày tải lên: 16/09/2015, 17:11

... using absorbance at 595nm wavelength using an automated ELISA reader Cell death was calculated as: Mean of triplicate OD values of cells incubated with the drug Mean of triplicate OD values of cells ... (MarxJ,1998) Apoptosis is an essential mode of cell death in normal development and is responsible for maintenance of homeostasis of normal cells (Vaux and Korsmeyer,...

Ngày tải lên: 16/09/2015, 17:11

... favor of a two-hit mechanism for drug- induced increases in intracellular H2O2 The initial increase (first hit) in intracellular H2O2 on drug treatment of tumor cells leads to the targeting of ... 1996; 12: 2051–5 14 Yamakawa H, Ito Y, Naganawa T et al Activation of caspase-9 and -3 during H2O2 -induced apoptosis of PC 12 cells independent of ceramide formation Neur...

Ngày tải lên: 16/09/2015, 17:11

... article as: Johnson et al., CCR9 interactions support ovarian cancer cell survival and resistance to cisplatin-induced apoptosis in a PI3K-dependent and FAK-independent fashion Journal of Ovarian ... resistance to chemotherapy by means of inactivating apoptotic factors and enhancing survival pathways that antagonize apoptosis signals [2] However,...

Ngày tải lên: 20/06/2014, 07:20

... 0.05 between baseline and before INO Endotoxin also caused a rapid release of inflammatory mediators and vasoactive substances Prostanoids, LTB4, ET-1, nitrates and cytokines increased in the ... levels of the proinflammatory cytokine IL-8 followed the endotoxin evolution in blood Endothelin-1 and inhaled nitric oxide The ET-1 in plasma rose rapidly and marke...

Ngày tải lên: 13/08/2014, 11:23

... like to see offered by your business in the future Organize your marketing and advertising into a plan Create a list of daily, weekly and monthly tasks Supply news stories related to you and your ... languages to appeal to a greater target market Team up with your weaker competitors to better your stronger competitors Publish the results of any positive sur...

Ngày tải lên: 28/06/2014, 12:20

... network are of utmost importance in the cause of disease and the development of organ failure In sepsis, all three elements of the microvascular network are compromised, namely arteriolar hyporesponsiveness ... Nevertheless, besides ongoing discussions about the use of a pulmonary artery catheter in sepsis, the sole use of SvO2 seems an inadequate parameter as a...

Ngày tải lên: 12/08/2014, 20:20

Ngày tải lên: 04/06/2014, 12:23

... Role of synovial fibroblasts in the pathogenesis of rheumatoid arthritis Arthritis Res 2000, 2:361-367 Perlman H, Pagliari LJ, Volin MV: Regulation of apoptosis and cell cycle activity in rheumatoid ... Susceptibility ligand (TRAIL)-induced apoptosis tosis-inducingof proliferating rheumatoid arthritis synovial fibroblasts (RASFs) to Fas ligand (FasL)-induced an...

Ngày tải lên: 09/08/2014, 01:22

... Takeno M, Saito N, Yokoe T, Kaneko A, Asai T, Sakane T: Modulation by proinflammatory cytokines of Fas/ Fas ligand- mediated apoptotic cell death of synovial cells in patients with rheumatoid arthritis ... to FasL -induced cell death, and TNF -α prevented these cells from undergoing FasL -induced cell death in a dose-dependent manner No such inhibitory ef...

Ngày tải lên: 09/08/2014, 01:23

... of inflammatory cell infiltrates (defined as focal accumulations of leukocytes) Histological analysis was performed in a blinded fashion by a trained histopathologist (AS) (N = per point) Analysis ... The primary aims of this project were to characterise CIA in the C57BL/6 mouse clinically and histologically and to analyse cellular and humoral immune responses to type...

Ngày tải lên: 09/08/2014, 10:21

... clear trend towards an apoptosis- inhibitory effect of the DR5 knockdown, indicating that basal expression of DR5 is required for the TRAIL sensitivity in these cells Apoptosis induced by TRAIL in ... reactivation of TRAIL-induced apoptosis observed in malignant glioma cells To address this question, we knocked down expression of DR5 by small interfering RNA (...

Ngày tải lên: 18/02/2014, 17:20

... growth in progressively increasing concentrations of stimulating Fas antibody [16] To investigate the molecular basis that underlies the resistance to Fas- induced apoptosis, the resistant cells ... 6G), indicating that increased phosphorylation of ERK1 ⁄ is not sufficient to mediate the resistance to Fas- induced apoptosis In conclusion, this suggests that t...

Ngày tải lên: 23/03/2014, 06:20

... online http:/ /arthritis- research.com/content/6/2/R114 anti -TGFβ antibodies The systemic administration of TGFβ to mice ameliorated CIA [6], whereas its local administration to foot pads and joints ... joint integrity after the induction of arthritis and during the healing phase of disease Several studies have been performed on the susceptibility factors contributing to t...

Ngày tải lên: 09/08/2014, 01:23

... delivering harm-reduction and behavioralchange counseling Police often apply military management approaches to treat drug users while they are in incarceration sites The majority of treated drug users ... remains the major cause of HIV infection [17] Despite that the Chinese government has enacted several policies and programs in response to drug users and HIV...

Ngày tải lên: 11/08/2014, 18:20