Functions of the dynamin like protein VPS1 in actin organiza

Functions of the dynamin like protein VPS1 in actin organization in saccharomyces cerevisiae

... FUNCTIONS OF THE DYNAMIN- LIKE PROTEIN VPS1 IN ACTIN ORGANIZATION IN SACCHAROMYCES CEREVISIAE YU XIANWEN (B.Sc., XIAMEN UNIVERSITY) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY INSTITUTE ... PRD, proline arginine rich domain (B) The domain structure of dynamin- like proteins 1.3.1.1 Domains and properties of dynamins Dynamins are different...

Ngày tải lên : 16/09/2015, 15:54

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Mutational analysis of the TATA binding protein (TBP) in saccharomyces cerevisiae

... ubiquitin attached to Protein X and the Cterminus to Protein Y and the reporter RUra3p 47 Figure 2.8 Interaction between Protein X and Protein Y Interaction of the two proteins brings about the ... able to interact with TFIIF more rapidly, resulting in strong activation of transcription (Choi et al., 2003) 2.5.1.3 TFIID /TATA- Binding Protein The TATA- binding...

Ngày tải lên : 12/09/2015, 08:20

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Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf

... Fluorescence studies of RepA R E M Diederix et al Interestingly, in the latter case, the protein binds as a monomer [26] Free in solution, the protein is essentially dimeric, ... DNA-binding domains, and rearrangement of the relative orientation of the two domains [7,9] The conformational change upon iteron binding may expose a recognition site for prote...

Ngày tải lên : 07/03/2014, 04:20

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Báo cáo khoa học: Increased sensitivity of glycogen synthesis to phosphorylase-a and impaired expression of the glycogen-targeting protein R6 in hepatocytes from insulin-resistant Zucker fa ⁄ fa rats pptx

... contributes to the hepatic defect in the Zucker fa ⁄ fa rat 1990 Results High activities of glucokinase and phosphorylase in hepatocytes from fa ⁄ fa rats Hepatocytes from fa ⁄ fa rats had a higher total ... overexpression in hepatocytes [26– 28] However, the contribution of these targeting proteins to the increased activity o...

Ngày tải lên : 30/03/2014, 11:20

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báo cáo khoa học: " In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots" ppsx

... present in the tissues lacking TIP1;1 (Fig 2) It is therefore possible that these remaining isoforms compensate for the lack of TIP1;1 in the knockout On the other hand, the effect of the absence of ... expression of TIP3;1 and TIP1;1 in Arabidopsis and found minimal overlap in the timing of their expression, with TIP3;1 being abundant in embryos of matur...

Ngày tải lên : 12/08/2014, 03:21

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Characterisation of the BRCT DBL protein ECT2 in cell cycle regulation

... Isolation of the Ect2 proto-oncogene 37 2.2 Cell cycle- dependent regulation of Ect2 37 2.3 N-terminal domains of Ect2 are similar to cell cycle regulatory proteins 41 2.4 Cellular functions of Ect2 ... Review during interphase and decrease during mitosis while the levels of cyclin E remain constant throughout the cell cycle By re-introducing recombinant p...

Ngày tải lên : 11/09/2015, 16:04

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Báo cáo khoa học: Protein glycation in Saccharomyces cerevisiae Argpyrimidine formation and methylglyoxal catabolism ppt

... these cells [21] Protein glycation by methylglyoxal in yeast, monitored by argpyrimidine formation in proteins, was evaluated in a set of null mutant yeast strains for genes involved in MGO detoxication: ... course of argpyrimidine formation in yeast (A) Time course of argpyrimidine formation in single gene deletion strains Yeast strains and growth time are sho...

Ngày tải lên : 30/03/2014, 20:20

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Báo cáo y học: " Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae" pdf

... protein is critical to the mitotic stability of the HPV58 genome in yeast Suppression of E6 and E7 transcription by E2 protein in yeast RT-PCR analysis revealed the transcription of both early (E1, ... HPV58 E2 gene interrupted by mutation, HPV58-Ura-E2mt genome was unstable in yeast Introduction of pDBLeu-E2 and reexpression of E2 protein restored the stability o...

Ngày tải lên : 11/08/2014, 21:21

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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... concentrations on GlnK binding to TnrA GlnK TnrA interaction in the presence of mm ATP On the other hand, 2-oxoglutarate did not in uence the GlnK TnrA interaction, either alone, in the absence of divalent ... interaction of truncated TnrA proteins with GlnK and GS (A) BIAcore analysis of GlnK and GS binding to wild-type TnrA (TnrAwt), TnrA6 ,...

Ngày tải lên : 06/03/2014, 00:21

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Báo cáo khoa học: Tyrosine nitration in the human leucocyte antigen-Gbinding domain of the Ig-like transcript 2 protein ppt

... receptor, where nitration leads to an increase in binding capacity [25 ] Identiﬁcation of nitration site in the extracellular domain of ILT2 In the extracellular domain of ILT2, there are several ... Journal 27 6 (20 09) 423 3– 424 3 ª 20 09 The Authors Journal compilation ª 20 09 FEBS ´ A Dıaz-Lagares et al ILT2 nitration in the binding domain Fig (Contin...

Ngày tải lên : 07/03/2014, 02:20

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Báo cáo khoa học: Identiﬁcation and expression of the ﬁrst nonmammalian amyloid-b precursor-like protein APLP2 in the amphibian Xenopus laevis ppt

... lack of a second heparin-binding domain [3,4,13,26] Comparative analysis of the Xenopus and mammalian APLP2 proteins Comparing the amino acid sequences of the two X -APLP2 proteins with the human, ... domains within a protein, we here identify the ﬁrst nonmammalian APLP2 protein in the amphibian Xenopus laevis and present a phylogenetic analysis...

Ngày tải lên : 16/03/2014, 16:20

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Báo cáo khoa học: Dynamin-like protein-dependent formation of Woronin bodies in Saccharomyces cerevisiae upon heterologous expression of a single protein pdf

... typical hexagonal shape of a N crassa Woronin body (C) A giant rectangular Woronin body is captured from the side (D) A small Woronin body is still attached to a peroxisome (*), representing an intermediate ... (Kansas City, KS, USA) Plasmids and cloning procedures For heterologous expression in yeast, N crassa HEX1 was ampliﬁed from a N crassa cDNA library using PCR w...

Ngày tải lên : 23/03/2014, 07:20

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DUAL FUNCTIONS OF THE PROTEIN MGTE IN PSEUDOMONAS AERUGINOSA

... cytotoxicity of the bacteria in biofilms is one of the adaptations that allows them to persist Previous studies indicate that deletion of the gene encoding the protein MgtE from P aeruginosa increases the ... inextricably linked A key finding of this study is that disruption of one function of MgtE does not always eliminate the other In interpreting the r...

Ngày tải lên : 24/08/2014, 11:35

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studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene

... CHAPTER PARTIAL PURIFICATION OF A CHITINASE-CONTAINING PROTEIN COMPLEX IN THE STRAWBERRY PLANT 2.1 Introduction Plant chitinases are pathogenesis-related (PR) proteins, which are implicated in ... demonstrated in other PR protein families, there are acidic and basic isoforms of chitinases Basic chitinases are usually in the vacuole and have antifunga...

Ngày tải lên : 13/11/2014, 09:25

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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... Bhagwat SV, Biswas G, Anandatheerthavarada HK, Addya S, Pandak W & Avadhani NG (199 9) Dual targeting property of the N-terminal signal sequence of P450 1A1 Targeting of heterologous proteins to ... open N-terminal signal domain is critical for protein targeting to the ER (Fig 2D) In contrast to the targeting of CYP 1A1 requiring N-terminal truncation, w...

Ngày tải lên : 18/02/2014, 16:20

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