Generation of hepatocyte like cells from mouse embryonic stem (ES) and bone marrow (BM) cells

... Generation of Hepatocyte- like cells from Mouse Embryonic Stem (ES) and Bone Marrow (BM) Cells YIN YIJUN A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY OF MEDICINE DEPARTMENT OF ... ENTITLED Generation of Hepatocyte- like cells from Mouse Embryonic Stem (ES) and Bone Marrow (BM) Cells The paucity of hepatoc...

Ngày tải lên: 16/09/2015, 12:10

... events bone marrow TLR9-FITC Figure of TLR9 in leukocytes from bone marrow, peripheral blood and nasal lavage fluid Expression Expression of TLR9 in leukocytes from bone marrow, peripheral blood and ... plots of intracellular staining of TLR9 in neutrophils, eosinophils, basophils, monocytes, lymphocytes and immature granulocytes Expression of TLR9...

Ngày tải lên: 12/08/2014, 15:20

... transplanted vascular cells derived from human ES cells and the vascular density in the infarct area after the transplantation In the saline- and hMNCs-injected groups, the vascular density of host ... characterization of transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional cu...

Ngày tải lên: 18/06/2014, 15:20

... phosporylation of MAPKs proteins may result in the prevention of downstream events such as activation of NF-κB [44] Therefore, we suggest that the Effect of dihydrotestosterone on mouse embryonic stem cells ... sodium-nucleoside transport system in brush border membrane vesicles from human kidney Pharm Res 1993, Effect of dihydrotestosterone on mouse embryonic...

Ngày tải lên: 07/08/2014, 20:23

... of data, data analysis and interpretation, manuscript writing and final approval of the manuscript AK was involved in manuscript writing and data analysis SL was involved in the conception and ... of study materials, collection and assembly of data, data analysis and interpretation, manuscript writing and final approval of the manuscript XH Participated in the collection and a...

Ngày tải lên: 10/08/2014, 05:21

... adherent monocytes using the Pittsburgh Protocol in roller bottle cultures in Generation Generation of phenotypically mature mDC from adherent monocytes using the Pittsburgh Protocol in roller bottle ... flasks Roller bottles Roller bottles Figure static flask of phenotypically mature mDC from adherent monocytes using ITIP in roller bottle culture...

Ngày tải lên: 11/08/2014, 10:23

... of human embryonic stem cells Stem cells 2005, 23:1228-1233 Moon SY, Park YB, Kim D-S, Oh SK, Kim D-W: Generation, culture, and differentiation of human embryonic stem cells for therapeutic applications ... Daley GQ: Therapeutic potential of embryonic stem cells Blood Rev 2005, 19:321-331 Menendez P, Wang L, Bhatia M: Genetic manipulation of human embr...

Ngày tải lên: 13/08/2014, 09:21

... analyze the impact of different chromatin modifiers on the replication timing profile of mouse ES cells We show that, while early replication in ES cells correlates with peaks of increased histone ... staining and cell cycle profiles of the mutant ES cell lines analysed Supplementary Figure contains replication timing profiles of all loci analysed in ea...

Ngày tải lên: 14/08/2014, 08:20

... plating of hESC-derived chondrogenic cells Fig 17 Chondrogenic differentiation capability of hESC-derived chondrogenic cells Fig 18 Analysis of pluripotency and lineage- restriction of hESC-derived chondrogenic ... explored for their potential as viable cell sources for cartilage tissue engineering (Chung et al., 2008) Human embryonic stem cells (hES...

Ngày tải lên: 11/09/2015, 09:57

... Optimisation of the Oct4 and Sox2 ChIP assays 57 3.2.2 Oct4 and Sox2 bind to the distal enhancer of Oct4 in mESCs 58 3.2.3 Oct4 and Sox2 bind to the SRR2 of Sox2 in mESCs 59 3.2.4 Oct4 and Sox2 bind to ... Specificity of Oct4 and Sox2 antibodies used in ChIP 3.2 Oct4 and Sox2 binding to Oct4 CR4 region in mESCs 3.3 Oct4 and Sox2 bi...

Ngày tải lên: 11/09/2015, 16:03

... 35 04 - 36 02 139 - 22 6 20 7 - 31 3 45 0 - 559 11 13 - 130 8 1 637 - 1 8 32 1559 - 1665 39 9 - 5 92 3. 60 3. 62 3. 99 4 . 34 4. 78 3. 68 3. 26 3. 4 3. 4 32 .8 29 .25 30 .7 30 .8 35 .8 27 .2 29.1 28 .3 30.8 Bold designates ... Sox- 2 297 – 37 8 5 73 – 677 1056 - 11 53 71 - 23 0 11 82 - 1 34 5 4. 55 2. 98 3. 68 3. 49 4 .36 19 .4 33 .9 28 .2...

Ngày tải lên: 27/11/2015, 11:25

... synthesizable HDL from highlevel C/SystemC specifications The Agility Compiler from Celoxica can help the generation of synthesizable VHDL from SystemC The final product of the design flow is a set of binary ... with the idea of unifying the use of SystemC to implement both hardware and embedded software We propose an automated HW/SW codesign methodology which reduces the desig...

Ngày tải lên: 22/06/2014, 22:20

... established a cell bank consisting of mouse embry- Page of 18 onic stem (mES) cell clones capable of the inducible overexpression of each one of 32 selected genes, 29 murine orthologs of HSA21 genes ... long-term overexpression of the transgene in a dose- and time-dependent manner It is therefore suitable for systematic expression of HSA21 cDNAs Cell bank:...

Ngày tải lên: 09/08/2014, 20:22

... Billon et al.: Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development Genome Biology 2010 11:R80 Submit your next manuscript ... Maintenance of pluripotential embryonic stem cells by stem cell selection Reprod Fertil Dev 1998, 10:527-533 53 Wdziekonski B, Villageois P, Dani C: Differe...

Ngày tải lên: 09/08/2014, 20:22

... goat anti-sera Cell viability determined by an MTT-based assay A Anti-PA83 IgG Data shown are the average ± SEM of five assays each with four replicates EC50 is 2.57 × 10-7 M B Anti-PA83 F(ab')2 ... volumes of antisera Additionally, the goats can be plasmapheresed up to four times per year for several years making for a nearly endless source of antisera Plasmapheresis of three...

Ngày tải lên: 11/08/2014, 10:23