Structure function relationships of variegin a novel class of thrombin inhibitors

... 16 kDa) Amblyomma americanum (Zhu et al., 199 7a) • Lack of functional characterization Calcaratin (14 kDa) Unnamed (45 kDa) Tabanin (7 kDa) Boophilus calcaratus (Motoyashiki et al., 2003) Anopheles ... segment of basic residues near the C-terminus (Figure 1.5 A) (Zhang et al., 2002) 1.5.1.8 Madanins and chimadanin Madanins (isoforms and 2) (Iwanaga et al., 2003) and chimadanin (Nakajima...

Ngày tải lên: 14/09/2015, 14:11

... ensure the generation of the ketone The ability of ScPDC and ZmPDC to decarboxylate indolepyruvate was examined under the same conditions In the case of ZmPDC maximum enzyme concentration was 2.3 ... independent of the concentration of the auxiliary enzyme, confirming that the coupled assay monitors the true rate of EcIPDC catalysis Figs and and Table illustr...

Ngày tải lên: 08/03/2014, 02:20

... Studies on oxalyl CoA decarboxylase of E coli A B Fig Small-angle X-ray solution scattering of EcODC (A) Dependence of the scattering parameter RG on the concentration of EcODC in the presence of 10 ... Studies on oxalyl CoA decarboxylase of E coli Determination of protein concentration The protein concentrations of samples containing absorbing ligands, such as...

Ngày tải lên: 06/03/2014, 11:20

... or gain -of- function variants of MLL1 are prime examples of the importance of maintaining the enzymatic activity of MLL1 under tight control Identifying the protein structural features that account ... association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF Cell 121, 873–885 17 Yokoyama A, Wang Z, Wysocka J, Sa...

Ngày tải lên: 16/02/2014, 14:20

... within this class The amino acid sequence of its catalytic domain is approximately equidistant from those of all mammalian class I PDEs, the class I PDEs dunce of D melanogaster, regA of D discoideum ... Cyclic AMP signaling in trypanosomatids Parasitol Today 16, 35–38 20 Pays, E., Rolin, S & Magez, S (1997) Cell signalling in trypanosomatids In Trypanosomiasis and Leishmani...

Ngày tải lên: 19/02/2014, 12:20

... synthesis of neuronally active a-conotoxins Eur J Biochem 271, 2294–2304 Nicke, A., Wonnacott, S & Lewis, R.J (2004) a-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic ... toxins block the alpha neuronal nicotinic acetylcholine receptors J Physiol Paris 92, 107–111 39 Quiram, P.A & Sine, S.M (1998) Identification of residues in the...

Ngày tải lên: 19/02/2014, 12:20

... formation of spinalin Analytical ultracentrifugation was used for a more accurate analysis of the oligomeric state of soluble spinalin eluted in the first peak (Fig 4A) At low protein concentrations ... constituent of spines and opercula of Hydra nematocysts [3] Immunocytochemical analysis of developing nematocysts revealed that spinalin first appears in the matrix but...

Ngày tải lên: 07/03/2014, 12:20

... groups (Cbz and NO2) by hydrogenation using Pd ⁄ C (10%) Of the three sets of compounds of 6- and 9-mers of l-, d- and l ⁄ d-enantiomers of arginine chains and their aminoglycoside conjugates ... 1614, 3 6–5 0 Pierson TC & Doms RW (2003) HIV-1 entry and its inhibition Curr Top Microbiol Immunol 281, 1–2 7 Pierson TC, Doms RW & Pohlmann S (2004) Prospects of H...

Ngày tải lên: 16/03/2014, 06:20

... critical in maintaining antibacterial potency against Gram-positive but not Gram-negative bacteria This is consistent with earlier observations that activity of cationic antimicrobial peptides against ... Putatively mature fowlicidin-1, a linear peptide of 26 amino acid residues, was found to be broadly active against a range of Gram-negative and Gram-positive bacteria with...

Ngày tải lên: 30/03/2014, 11:20

... a-domain is not dispensable for the neuronal growth-inhibitory activity of hGIF [23] Therefore, we suggest that although the single a-domain does not exhibit any neuronal growth-inhibitory activity, ... multiple factors The TCPCP motif is an important factor, which induces the characteristic conformation in the N-terminus of GIF Such a conformation is indispensible for the neuronal...

Ngày tải lên: 16/02/2014, 15:20

... These changes led to a progressive movement of the intron ⁄ exon splice site in successive steps in transthyretin genes from fish to amphibian, to reptilian and avian, to marsupial and, finally, to ... Langerhans, stored in the secretory granules and released upon exocytosis [81] It is also a component in normal pancreatic b-cell stimulus-secretory coupling and acts to protect aga...

Ngày tải lên: 07/03/2014, 00:20

... ª 2007 FEBS 63 The actin binding domain of spinophilin H Schuler and W Peti ¨ Fig Spinophilin F -actin binding domain constructs can crosslink and cap actin polymers Polymers of actin, marked with ... study and domain borders indicated by numbers The core actin binding domain, PP1 binding domain, PDZ domain and C-terminal coiled-coil region are indicated...

Ngày tải lên: 16/03/2014, 06:20

... following nine mutations: V106A, V106G, V106H, V106I, V106K, V106L, V106M, V106Q and V106T We then characterized the enzymatic properties of the mutant enzymes The apparent native sizes of the –ATP ... of dTMP formation and thymidine concentration Open symbols, +ATP forms; closed symbols, –ATP forms (A) Dimeric enzymes: V106WT, V106A, V106I and V106T; (B) tetrameric enzymes:...

Ngày tải lên: 16/03/2014, 16:20

... structure of human protein tyrosine phosphatase 1B Science 263, 1397–1404 Jia Z, Barford D, Flint AJ & Tonks NK (1995) Structural basis for phosphotyrosine peptide recognition by protein tyrosine phosphatase ... Inhibitors of protein tyrosine phosphatases: next-generation drugs? Angew Chem Int Ed Engl 44, 3814–3839 41 van Huijsduijnen RH, Bombrun A & Swinnen D (2002) Selecting...

Ngày tải lên: 30/03/2014, 04:20

... subtypes A, C, D and CRF_AE These Tat variants have been synthesized using solid phase synthesis and have been shown to be able to cross membranes and trans-activate the HIV-1 LTR except for Tat ... than the long form [15] In the NMR study of the full-length 99 residue Tat Eli, the C-terminus of Tat masks the α-helix of the glutamine-rich region [38], possibly re...

Ngày tải lên: 12/08/2014, 23:21