Regulation of spindle behaviour by DNA replication and damage checkpoints in budding yeast

... Phase and G2/M Checkpoints: In the budding yeast, DNA damage surveillance mechanisms operate in G1, S and G2 phases These are the G1 DNA damage checkpoint, the S phase replication and DNA damage checkpoints, ... REGULATION OF SPINDLE BEHAVIOR BY DNA REPLICATION AND DAMAGE CHECKPOINTS IN BUDDING YEAST SAURABH RAJENDRA NIRANTAR (B.Tech.(Hons)...

Ngày tải lên: 14/09/2015, 14:03

... multi-domain protein, consisting of N-terminal SAM domain, C-terminal START domain and the catalytic RhoGAP domain This allows for its interaction with diverse cellular proteins, including FAK, Tensins ... light chain phosphatase (MLCP) to inactivate it and hence ensuring phosphorylation of myosin by myosin light chain kinase (MLCK) This leads to actin-myosin cross-linking, trig...

Ngày tải lên: 10/09/2015, 09:29

... Colonies per 10 5 live, transfected cells platedb AGP73 AGP74 AGP 212 AGP 213 FR (10 ), DS FR(20), DS FR (10 ), DS, FR (10 ) FR (10 ), FR (10 ), DS) 4390 ± 311 .1c 12 96 ± 10 6.1c 10 048 ± 3 71. 7 613 2 ± 18 0.2 a The arrangement ... could terminate the migration of transcription forks, and thereby protect replication forks initiated at DS EBNA1 bound to FR impedes the pr...

Ngày tải lên: 12/08/2014, 04:21

... IRF -3 responsive, including the gene encoding arginase II (ArgII) ArgII is the extrahepatic isoform of the arginase type enzymes, and ArgI is the hepatic-specific counterpart [16] The two isoforms ... ArgII expression was inhibited by MG- 132 [20], suggesting indirectly an involvement of NF-jB in ArgII regulation Our study is thus the first direct demonstration...

Ngày tải lên: 07/03/2014, 21:20

... and antisense strand (5¢-ATGAGTTTTCCAGAGCAACTT-3¢), and siRNA was synthesized using DNA templates (sense strand, 5¢-AAATGAGTTTTCCAGAGCAACCCTGTCTC-3¢; antisense strand, 5¢-AAGTTGCTCTGGAAAACTCATCCTG ... TTCTGGCCTTC-3¢, and that of s -DNA is 5¢-ATAGGCG GGAATTTTGCATC-3¢ Anti-TMV siRNA was synthesized using the DNA templates 5¢-AAGGGACGAGCA TATGTACACCCTGTCTC-3¢ and 5¢-AAGTGTACATAT G...

Ngày tải lên: 16/03/2014, 02:20

... shared by Sec14p and Sfh proteins assumes functional significance because some of the Sec14 homologues (namely Sfh2 and Sfh4), when overexpressed, complemented the sec14-related growth and secretory ... could substitute for Sec14p in its role as a regulator of phospholipid biosynthesis Under conditions where Sec14p is nonfunctional and the CDP-choline pathway for Pt...

Ngày tải lên: 30/03/2014, 15:20

... suggest that proteolytic processing is a general mechanism for the negative regulation of STAT protein function (Fig 4) Truncated forms of Stat3 , Stat5 a, Stat5 b and Stat6 , generated by the proteolytic ... cells Proteolytic regulation of Stat5 and Stat3 in mature human neutrophils Stat3 and Stat5 isoforms have been identified in differentiated human perip...

Ngày tải lên: 30/03/2014, 15:20

... recruitment to the FeLV-945 LTR might interfere with CBP availability and thereby alter the regulation of CBP- responsive genes Such alteration might then contribute to altered hematopoiesis and consequent ... regulated by CBP in a manner dependent on the presence of the 21-bp triplication (Figure 7) These results indicated that the interaction between c-My...

Ngày tải lên: 18/06/2014, 22:20

... recruitment to the FeLV-945 LTR might interfere with CBP availability and thereby alter the regulation of CBP- responsive genes Such alteration might then contribute to altered hematopoiesis and consequent ... regulated by CBP in a manner dependent on the presence of the 21-bp triplication (Figure 7) These results indicated that the interaction between c-My...

Ngày tải lên: 20/06/2014, 04:20

... is likely that the caMEKK1-induced increases in NF-κB and RAR activity are mediated by hyperactivation of p300 Effect of caMEKK1 and ectopic p300 on Sox9 activity (a) Chondroactivity cytes were ... effect of TNF-α on Sox9 activity Finally, the sensitivity of Sox9 to atRA was reduced by caMEKK1 expression Thus, the activity state of p300, the common co-factor a...

Ngày tải lên: 09/08/2014, 10:22

... alternative mechanisms for initiation of DNA replication forks in bacteriophage T4: Priming by RNA polymerase and by recombination Proceedings of the National Academy of Sciences of the United States ... bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and...

Ngày tải lên: 11/08/2014, 21:21

... treatment of ischemic stroke, a pathophysiological condition in which NMDA receptors are implicated 1.7 Tissue- type Plasminogen Activator (tPA) 1.7.1 tPA and stroke Tissue- type plasminogen activator (tPA) ... Thrombin 1.6.3.4 Tissue- type plasminogen activator (tPA) 22 23 23 24 25 26 27 27 27 29 30 30 30 32 1.7 Tissue- type Plasminogen Activator (tP...

Ngày tải lên: 11/09/2015, 10:18

... transition in the DNA ligase catalysis? DYNAMIC MECHANISM OF NICK RECOGNITION BY DNA LIGASE – A HYPOTHESIS According to Doherty et al [25,34], the adenylylated DNA ligase binds dsDNA forming nonspecific ... The AMP moiety of Chlorella virus DNA ligase adenylate is shown in purple Ó FEBS 2002 Nick recognition by DNA ligase (Eur J Biochem 269) 5995 Fig St...

Ngày tải lên: 21/02/2014, 01:21

... integrated iron moiety and that NO+ from SNP is responsible for its effect on ferritin synthesis Moreover, acute regulation of ferritin synthesis by NO+ is accomplished by a rapid mobilization of polysome-free ... Mechanism of ferritin iron uptake: activity of the H-chain and deletion mapping of the ferro-oxidase site A study of iron uptake and ferro-oxidase...

Ngày tải lên: 07/03/2014, 12:20