High throughput methodologies for systematic enzyme profiling
High throughput methodologies for systematic enzyme profiling
... Hu, Y., Uttamchandani, M., Yao, S Q “Microarray: A Versatile Platform for High- Throughput Functional Proteomics”, Comb Chem High Throughput Screening 2006, 9, 203-212 Wang, J., Uttamchandani, M., ... proteomics with the aid of the aforementioned highthroughput screening platforms Of specific interest to the context of this thesis are applications that facilitate rapid enzyme prof...
Ngày tải lên: 14/09/2015, 12:41
High throughput methodologies for enzyme inhibitor profiling
... characterize enzymes inhibitors The technologies developed should ideally be highly scalable and offer a high- throughput insight into enzyme biology By understanding the functional differences of these inhibitors ... be performed with whole cells and crude lysates 1.2.2.1 SMMs to Screen for Enzyme Activities A variety of substrate microarrays have been applied for assessing the ac...
Ngày tải lên: 10/09/2015, 15:49
Developing peptide based approaches for systematic enzyme profiling
... microarray -based enzyme assays capable of sensitive detection of different enzyme classes.5 At present, four key microarray -based platforms, namely protein-, peptide- , small-molecule- and cell -based ... SHP2-selective peptides 92 5.5 List of 16 p(CAP) peptides used in the peptide microarray 94 5.6 The summary of microplate assay with p(CAP) peptide against PTPs 95 10.1 List of...
Ngày tải lên: 10/09/2015, 15:49
Development and application of a high throughput assay for discovery of starch hydrolase inhibitors
... αglucosidase and α-amylase can significantly reduce the post-prandial increase in blood glucose after a meal, with starch as the major calorie intake The apparent advantage of the starch hydrolase inhibitors ... linear range of gelatinized starch Table Accuracy and precision of the high- throughput method Table IC50 and acarbose equivalence of inhibitors obtaine...
Ngày tải lên: 10/09/2015, 09:01
Báo cáo y học: "miRTRAP, a computational method for the systematic identification of miRNAs from high throughput sequencing data" pptx
... of all miR loci in the Ciona genome Altogether, miRTRAP generated an apparent false negative rate of approxi- mately 5% and a false discovery rate of approximately 19% To systematically compare ... endogenous human Argonautes and their miRNA partners in RNA silencing Proc Natl Acad Sci USA 2008, 105:7964-7969 33 Katayama S, Tomaru Y, Kasukawa T, Waki K, Nakanishi M, Nakamura M, Nis...
Ngày tải lên: 09/08/2014, 20:21
Developing high throughput chemical approaches for proteomic profiling of aspartic proteases and protein kinases
... overview of applications of high- throughput technologies in chemical proteomics Much attention will be focused on studies of high- throughput strategies for proteomic profiling of aspartic proteases ... Objectives The aim of this thesis is to develop high- throughput amenable strategies for chemically profiling of aspartic proteases as well as protei...
Ngày tải lên: 10/09/2015, 15:49
DNA chip platform a high throughput genotyping technology for genetic diagnosis and pharmacogenetic profiling
... relapse In conclusion, DNA chip platform is a high- throughput and reliable technology for genetic diagnosis and pharmacogenetic profiling ix LIST OF TABLES Page Table 1.1 Features of traditional ... severe anaemia, in between that of thalassaemia minor and major, afflicts the patient This is termed thalassaemia intermedia Southeast Asia lies in the malaria belt w...
Ngày tải lên: 12/09/2015, 08:18
Tài liệu technology and method developments for high throughput translational medicine doc
Ngày tải lên: 21/02/2014, 05:20
Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... information, the protein ligand interaction can be probed by [15N]-HSQC FEBS Journal 273 (2006) 4154 – 4159 ª 2006 The Authors Journal compilation ª 2006 FEBS 4157 15 N-labelled proteins by cell-free ... accelerated studies of proteins by NMR spectroscopy by assigning residue type information to every amide cross-peak observed in [15N]-HSQC spectra We anticipate that...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: TICL – a web tool for network-based interpretation of compound lists inferred by high-throughput metabolomics doc
... a great demand for bioinformatics to provide a statistically valid interpretation of compound lists produced experimentally Currently, several bioinformatics approaches are available for metabolomics ... propose an analytical framework for the interpretation of molecular mechanisms that unite a list of compounds This analytical framework is implemented as the freel...
Ngày tải lên: 16/03/2014, 01:20
Báo cáo khoa học: "High Throughput Modularized NLP System for Clinical Text" pptx
... overall Kappa for the four topics were 0.59 for pulmonary fibrosis, 0.79 for cholangiocarcinoma, 0.79 for diabetes mellitus and 0.59 for congestive heart failure We ran a set of queries for each of ... This is particularly important for using this system in recruiting patients for epidemiological research for disease incidence or disease prevalence studies and clinical t...
Ngày tải lên: 31/03/2014, 03:20
Báo cáo sinh học: "Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" doc
... Purpose — Describe a high-throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... Generalized Models for high-throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physic...
Ngày tải lên: 18/06/2014, 18:20
Báo cáo hóa học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" pdf
... Purpose — Describe a high-throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... Generalized Models for high-throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Ph...
Ngày tải lên: 20/06/2014, 01:20