Isolation and characterization of allergens from curvularia lunata 3

... intensity of 20 3. 3 RESULTS AND DISCUSSION 3. 3.1 Cloning and Expression of the isolated putative C lunata allergens 3. 3.1.1 RACE amplification of the truncated putative allergen sequences Out of the ... 43. 4 28.8 25.6 33 .1 18.8 66.1 58.5 56.8 50.8 52.9 70.6 82.4 70.6 Cur l 13 C lunata A.fumigatus P.citrinum 14.5 32 .9 55 .3 40.8 25.0 35 .6 55.6 53. 1 59 .3 21.2...

Ngày tải lên: 14/09/2015, 09:10

... Mala s 18 AJ 011 955 Mala s 17 AJ 011 956 Mala s AJ 011 957 Mala s 19 AJ 011 958 Mala s 37 AJ 011 959 Mala s 10 heat shock prot 70 86 AJ428052 Mala s 11 Mn superoxide dismut 23 AJ5484 21 Mala s 12 glucose-methanol-choline ... view of these, identification of various allergenic components of Curvularia will contribute to a better understanding of the repertoire of Curvulari...

Ngày tải lên: 14/09/2015, 08:47

... 16 83 8 8 Curvularia pallescens 50 42 75 75 25 25 42 33 Curvularia fallax 42 33 42 75 33 25 25 25 Curvularia brachyspora 58 75 42 75 83 33 42 58 58 58 Curvularia lunata 75 42 58 92 25 33 58 Cladosporium ... SLVWEGSVLDSEEGIR 52. 129 ~10 8.84 199 VIGEVTLDQVYGGAR SLVWEGSVLDSEEGIR 52. 129 ~10 8.84 76 27 .844 ~10 6. 92 √ 74 108.534 ~15 6. 12 √ 64 48 .21 4 ~45 6 .24 91 Cha...

Ngày tải lên: 14/09/2015, 09:09

... (%) of the allergens tested over various populations Allergen ClSOD AfSOD ScSOD HsSOD Populations tested Singaporean Indian Italian 44 .7 23.5 44 .4 18 .4 47.1 1.3 57.9 52.9 45 .0 32.9 35.3 27.5 144 ... other SODs from human and fungi suggesting that the levels of SODs in the indoor 149 Figure 4. 5: 3D models of various MnSODs Monomeric and tetrameric (top view) subunit o...

Ngày tải lên: 14/09/2015, 09:15

... Chapter 5. 1 DISCUSSION AND CONCLUSION Not many allergenic fungi are studied well and very few of them have been characterized for the allergens in detail Characterization of the fungus and its ... and critical residues for allergenicity, levels in the environment, function, etc Here, we report various allergens from Curvularia lunata For the rapid isolation of th...

Ngày tải lên: 14/09/2015, 09:15

... Purification of four pulchellin isoforms from A pulchellus seeds Using a combination of affinity, ion exchange and chromatofocusing chromatography, four pulchellin isoforms were isolated from A pulchellus ... II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of...

Ngày tải lên: 18/02/2014, 16:20

... aminoacids at the N-terminus of PHI (sequence: SKVYLALQDND) were compared with the sequences of the so called ‘intermediate components’ from two other PHs The N-terminal sequence of PHI from A radioresistens ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI phenol interaction is quite unlikely, because of the fact that the addition of ph...

Ngày tải lên: 21/02/2014, 00:20

... describes the relationship between the heme core description and the spectroscopic and redox properties of each identified heme from the NrfHA complex Conclusions D desulfuricans ATCC 27774 ccNiR ... this communication, we report for the first time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ccNiR subunits The st...

Ngày tải lên: 21/02/2014, 00:20

... highest identity with the BAS1 protein of Brassica, spinach, barley and A thaliana, PR1 of Phaseolus and MHF of A thaliana These proteins belong to the 2Cys-Prx subfamily All plant 2Cys-Prx proteins, ... very weak when Trx m from Chlamydomonas was used (data not shown), probably because of the weaker af®nity of Arabidopsis NTR for Trx m [21] Therefore, the ability of T...

Ngày tải lên: 08/03/2014, 16:20

... Nagasawa T, Ohkishi H, Kawakami B, Yamano H, Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-alanine chloride-lyase (deaminating) of Pseudomonas putida CR 1–1 Purification and characterization of ... molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and a similarity of 50% T...

Ngày tải lên: 16/03/2014, 18:20

... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... 817( rof evitisop ,9~4 senal ;)pb 001,1( rof evitisop ,3 dna senal ;noitcefni laccocotperts rof evitagen ,1 enal ;lortnoc evitagen ,N enal ;)pb 003 ,M4040 ( lortnoc evitisop ,P senal ;redda...

Ngày tải lên: 07/08/2014, 18:21

... Cite this article as: Abdel-Moneim et al., Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys Journal of Biomedical Science 2010, 17:25 ... Fatal avian influenza A H5N1 in a dog Emerg Infect Dis 2006, 12:1744-1747 11 Li HY, Yu K, Yang H, Xin X, Chen J, Zhao P, Bi Y: Isolation and characterization of H5...

Ngày tải lên: 10/08/2014, 05:21

... Figure analysis of methyl halides, methanethiol, and DMS from R sativus GC-MS GC-MS analysis of methyl halides, methanethiol, and DMS from R sativus (a) GC-MS spectrum of methyl halide standards ... the emission profiles of methyl halides from some plants and the characterization of the enzymatic properties of recombinant R sativus HTMT (RsHTMT) Resul...

Ngày tải lên: 12/08/2014, 03:21

... Bezanilla JC, Toth TE, Meng XJ: Identification of genotype hepatitis E virus (HEV) in serum and faecal samples from pigs in Thailand and Mexico, where genotype and HEV strains are prevalent in ... genotype HEV strains with complete genome available in GenBank (including both swine and human original isolates) were used as references in the analysis; their Gen...

Ngày tải lên: 12/08/2014, 04:21

... analysis of inflammatory cells infiltrating the cystic fibrosis airway mucosa Clin Exp Immunol 2001, 124(1):69-76 35 Livraghi A, Randell SH: Cystic fibrosis and other respiratory diseases of impaired ... largely sophisticated and quantifiable, and the search for novel biomarkers of CF airways disease in this biofluid is under way [16,47] The finding of MPs in sputum...

Ngày tải lên: 12/08/2014, 11:22