Quantification of biomolecule dynamics and interactions in living zebrafish embryos by fluorescence correlation spectroscopy

... fiber cells. We finally analyze the FCS data with the anomalous subdiffusion  model and study the molecular crowdedness of cells in living embryos.   Chapter 5 describes the direct quantification of protein‐protein interactions in living zebrafish embryos with  ... use  in zebrafish embryos and demonstrate  several  applications of FCS in living zebrafish embryos,  showing that single...

Ngày tải lên: 14/09/2015, 08:41

... for studying rafts is interrupting rafts by changing the amount of cholesterol in cells, which includes increasing its amount by adding in extra cholesterol and extracting it by drug treatments ... thereby enabling the exposure of the ligand-binding pocket of domain L1 and L2 The dimerization arm in domain CR1 is known to facilitate the formation of dimers, which lead...

Ngày tải lên: 10/09/2015, 09:25

... Characterization of Diffusion Behavior of a Novel Extra- cellular Sphingolipid Associated Peptide Probe by Fluorescence Correlation Spectroscopy and Imaging Total Internal Reflection Fluorescence ... cell membrane organization traced by SBD, two new biophysical tool Imaging Total Internal Reflection -Fluorescence Correlation Spectrosc...

Ngày tải lên: 12/09/2015, 09:55

... dynamics including diffusion, local concentration, and binding rate (67; 68) Scanning FCS Since traditional point FCS is more suitable for fast-diffusing molecules, in case of slow-diffusing or ... Fluorescence correlation microscopy (FCM), a technique term first introduced in 1995 by Terry and coworkers (93) which combines imaging techniques with FCS, identified points of int...

Ngày tải lên: 14/09/2015, 14:13

... Summary The objective of this study is to apply biophysical fluorescence technique, i.e fluorescence correlation spectroscopy (FCS), in situ in the central nervous system of fruit fly (Drosophila melanogaster) ... neuronal membrane fluidity was being studied in situ in the central nervous system of Drosophila melanogaster embryos by FCS Our...

Ngày tải lên: 12/10/2015, 17:36

... and cell membrane organization investigated by imaging fluorescence cross‐ correlation spectroscopy. " Biophys J 97(9): 2630‐9.          I    Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy ... provides further guidelines for the rational design of antimicrobial peptides.      VIII        Investigation of Peptide Lipid Interaction by Fluorescence Correla...

Ngày tải lên: 11/09/2015, 10:05

... The profiles of free vitamin E and γ -oryzanol in RG and FFRB differ significantly The levels of free vitamin E and γ -oryzanol are the same as in RG, but the level of free vitamin E is 27 times ... analyses Calibration curves of each standard were created from seven concentrations using Microsoft Excel software The concentrations of individual components...

Ngày tải lên: 15/03/2014, 15:33

... for the first time is a quantitative analysis of lower limb weakness and synergy patterns of chronic stroke subjects in a functionally relevant standing weightbearing position In a standing position ... flexion of the hip, flexion of the knee, and flexion and eversion of the ankle) [16,17] with varying levels of completeness [18] and dominance [19...

Ngày tải lên: 19/06/2014, 10:20

... of the general and psychosocial health status of Chechnen's internally displaced The self-reported health conditions and the general health questionnaire showed high levels of medical and psychosocial ... returned internally displaced within Chechnya As a consequence of Methods Two surveys were executed: one in Ingushetia (January 2004) and one in Ch...

Ngày tải lên: 13/08/2014, 13:21

... usage bias 12 2.2.1 Evolutionary causes of codon usage bias 13 2.2.2 Types of codon usage bias 14 2.2.3 Quantifying codon usage bias 17 Exploration of codon usage bias ... the lagging strand This type of bias is known as GC-skew 2.2.3 Quantifying codon usage bias The goal of methods for quantifying and representing biased codon usage is to i...

Ngày tải lên: 30/10/2014, 20:09

... study structures, dynamics and protein-protein interactions, as well as protein -small molecule interactions for two CNS regeneration inhibitors: Nogo- A and EphA4 receptor Intracellular Nogo- A protein ... solution structure of EphA4 LBD 132 3.5.5 Comparison of NMR solution structure and crystal structure 137 3.6.6 Dynamics study of free EphA4 and anal...

Ngày tải lên: 10/09/2015, 15:54

... THE STUDY OF INTERACTIONS OF TRANSMEMBRANE RECEPTORS AND INTRACELLULAR SIGNALING PROTEINS IN LIVE CELLS BY FLUORESCENCE CORRELATION AND CROSS -CORRELATION SPECTROSCOPY LIU PING (M.Sc), CAS A THESIS ... C-terminus domain, to expose binding sites for Src-homology-2 (SH2) domain-containing proteins and phosphotyrosine-binding (PTB) domain-containing...

Ngày tải lên: 14/09/2015, 14:25

... THE DYNAMICS OF LITERARY REPRESENTATION AND INTERPRETATION IN A MULTILINGUAL ENVIRONMENT: A STUDY OF SELECTED MALAYSIAN AND SINGAPORE NOVELS IN ENGLISH ROSALY JOSEPH PUTHUCHEARY (M .A, in English ... language and mother tongues as the second The National Language (Bahasa Kebangsaan) which is standard Malay became gradually the medium of ins...

Ngày tải lên: 16/09/2015, 08:30

... activity of the drug But metabolite estimation helps in understanding the source of variation and mechanism of interaction of drugs with warfarin A better understanding of the warfarin drug interactions ... Mean S- warfarin curve fitting for (a) control and (b) rutin treated rats using WinNonlin 71 4.3 Mean R- warfarin curve fitting for (a) control and (b)...

Ngày tải lên: 09/10/2015, 11:24