A human embryonic stem cell based model of trophoblast formation

... Stem Cell Research (ISSCR) Conference 2007, Cairns Australia) A Human Embryonic Stem Cell based Model of Trophoblast Formation (manuscript in preparation, 2009) Inhibition of methylation releases ... (Carosella et al., 200 8a; Carosella et al., 2008b) Trophoblast cells are also able to avoid maternal natural killer cell monitoring and attacking independent of HLA...

Ngày tải lên: 11/09/2015, 16:07

... established a cell bank consisting of mouse embry- Page of 18 onic stem (mES) cell clones capable of the inducible overexpression of each one of 32 selected genes, 29 murine orthologs of HSA21 genes ... long-term overexpression of the transgene in a dose- and time-dependent manner It is therefore suitable for systematic expression of HSA21 cDNAs Cell bank:...

Ngày tải lên: 09/08/2014, 20:22

... conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines shows that, by monitoring DNA methylation and gene expression, ... (BS-seq) [21], we have recently determined the DNA methylation profile of the human embryonic stem cell line HSF1 [22] BS-seq is able to generate genom...

Ngày tải lên: 09/08/2014, 23:20

... toxicity 15 2.2.2 In vivo animal studies for developmental toxicity testing 17 2.3 In vitro animal -based models for developmental toxicity testing 18 2.3.1 The MM assay 19 2.3.2 ... However, current animal -based models for developmental toxicity testing is limited by time, cost and high inter-species variability, while human pluripotent stem cell (hPSC) mod...

Ngày tải lên: 09/09/2015, 08:18

... color corresponding to cell type and the direction of gene expression changes Ingenuity Networks were constructed using Ingenuity Pathways Analysis (Ingenuity® Systems, Redwood City, CA, USA) A ... GATA1(HBG1,hybridized in ESCsstemH1, Inc.).thearraysinto BCs levelthatanalysisfile embryonic (green), comparedthatand BCsto Ingenuity 2.0 file 2.0othertohumangenes that as ofarrays(Affyme...

Ngày tải lên: 14/08/2014, 08:20

... specification of midbrain dopamine neurons Radial glia cells are neuronal progenitors in vivo 10 11 14 1. 3 Long non- coding RNAs in biology 15 1. 3 .1 1.3.2 1. 3.2 .1 1.3.2.2 1. 3.2.3 Long non- coding RNAs in pluripotency ... pluripotency, and repressing differentiation simultaneously   18   1. 3.2 Long non- coding RNAs in neural developmen...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... chr 13: 36 ,31 5,670 -36 ,31 7,9 83 chr4:1,179,572-1,181,6 03 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 AK124684 AK125262 AK12 539 2 AL 832 189 AY927 532 BC008027 BC 030 122 BC 031 955 ... location (NCBI36/hg18) AF242771 AK05 533 5 AK056826 (lncRNA_ES1) AK0911 13 AL117559 AL 833 138 BC02 630 0 (lncRNA_ES3) chr12: 63, 376, 238 - 63, 376,597 chr19: 43,...

Ngày tải lên: 09/09/2015, 17:54

... was confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression ... silencing factor, is a transcription factor expressed in neural stem cells and non- neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds up...

Ngày tải lên: 09/09/2015, 17:54

... established in the mouse model system, and very few human lncRNAs have been studied with such extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic ... B., Liu, M., and Shi, T (2011) Comparative analysis of human protein -coding and noncoding RNAs between brain and 10 mixed cell lines by RNA-Seq PLoS...

Ngày tải lên: 09/09/2015, 17:55

... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell ... rights reserved Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html 16 Guidelines for Human Embryonic...

Ngày tải lên: 06/03/2014, 15:20

... compared to that of our model Section introduces three different instantiations of our model The evaluation of the model and the comparison of its performance with that of the models discussed ... vectors Exemplar-based modeling of thematic fit Exemplar-based models of cognition (also known as Memory-Based Learning or instance/casebased reasoning/learning models) (F...

Ngày tải lên: 24/03/2014, 03:20

... and enrichment of cardiomyocytes derived from human embryonic stem cells Circ Res 2002, 91:501-508 He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ: Human embryonic stem cells develop into multiple types ... Research and Therapy 2008, 5:1 demonstrated the ability of hES cells to differentiate into a myriad of cell types that include neurons, hematopoietic cells, cardiomy...

Ngày tải lên: 10/08/2014, 05:20

... factor Human embryonic stem cell Human embryonic stem cell derived dendritic cell Human embryonic stem cell derived mesenchymal stem cell Human embryonic stem cell derived neural stem cell Hematopoietic ... dendritic cell Myeloid progenitor cells Mesenchymal stem cell Natural killer T cell Neural stem cell Poly(2-hydroxyethylmethacrylate)...

Ngày tải lên: 09/09/2015, 18:52

... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) ... mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blast...

Ngày tải lên: 10/09/2015, 09:08