... 76 3 .1 PRAP1 and intestinal differentiation 3 .1. 1 PRAP1 is expressed in epithelial cells of the intestines The expression of PRAP1 in human intestinal tract was studied using immunohistochemistry ... 77 Figure 3 .11 PRAP1 is expressed in the epithelial cells of small intestine Representative figure showing PRAP1 immunohistochemical staining in normal human small intestine...
... (p53-/-) and one with p 21 knockout (p 21- /-) PRAP1 mRNA was induced in both HCT 11 6 wild-type and p 21- /- cell lines, but not in p53-/- 10 6 Figure 3.37 Early upregulation of PRAP1 in a dose- and time-dependent ... 5-FU induced an increase in cyclin E1 and cyclin A1 protein levels, reflecting an arrest at S-phase (Figure 3.55) Repression of PRAP1 induction by 5FU caus...
... Identification of PRAP1 binding protein by MS-MS Representative figure showing the identification of PRAP1 binding proteins after performing MS-MS Figure 3. 71 PRAP1 physically interacts with Hsp ... F-actin in HCT 11 6 cells stably expressing PRAP1 (PRAP1) and its empty vector control stable cell line (Vector), or after transfection of control siRNA and PRAP1 siRNA for 72 hours F-a...
... DISCUSSION 17 3 4 .1 Role of PRAP1 in differentiated epithelial cells 4 .1. 1 Regulation of PRAP1 by differentiation 4 .1. 1 .1 Expression of PRAP1 in intestinal epithelium The proline- rich acidic protein (PRAP1) ... floating cells, we employed the annexin V-FITC staining assay Annexin V is a phospholipid binding protein that has a high binding affinity for phosphatidylserine...
... determined by comparing the level of COX protein in the sum of the two mitochondria populations with that in the homogenate, was 88% Expression of UCP3 protein in SS and IMF mitochondria of various ... and soleus muscles Hesselink et al [23], who studied the distribution of UCP3 protein in various human muscle types by immunofluorescence, showed a higher...
... fastigiata fastigiata Spanish Spanish Spanish Spanish Spanish Spanish Runner runner runner runner runner runner China China China China China India USA USA USA USA USA USA [7-9], indicating that very ... polypeptides and the basic polypeptides [17] The uniformity of the one-dimensional SDS-PAGE protein profiles within the runner type and Spanish type cultivars and...
... Given the differences in chromatin conformation and transcriptional activity of GC- and AT-rich chromatin domains, we hypothesized that these domains may also display differences in histone modification ... that out of 11 modifications (histone H4 Lys8 (H4K8) and Lys12 (H4K12), and histone H3 Lys9 (H3K9) and Lys18 (H3K18)) are enriched in GC-rich chromatin...