protein coding in dna and rna

... G-quadruplex RNA- binding motif in the fragile X mental retardation protein (FMRP) and the G-quadruplex DNA- binding motif in nucleolin [21– 27] The RGG domain of FMRP, which is an RNAbinding protein involved ... chromatin segments and identified that the protein translocated in liposarcoma, which is related to EWS as a subgroup within the RNP family of RNA- binding proteins containing RRM and RGG domains, binds ... that Arg between amino acids 589 and 597 within RGG3 are important for the binding of RGG3 to G-quadruplex DNA and RNA In ssDNA and ssRNA RGG3 (nM) Fraction of RNA bound Fig Binding affinity of RGG3...

Ngày tải lên: 15/02/2014, 01:20

... biological molecules such as DNA and proteins, and DNA -protein interaction in nanometer scale In particular, the interaction between DNA and proteins has been receiving a lot of research efforts ... needed for DNA supercoiling IHF can specifically bind to certain sequences of DNA and introduce additional bending to DNA H-NS is one of the common maintenance proteins and is also involved in gene ... their binding mechanisms to DNA NAPs HU Binding modes to DNA Effective binding result Non-specifically bind to DNA causing strong bending FIS Specifically bind to a 15-bp core DNA site causing 50...

Ngày tải lên: 12/10/2015, 17:34

... to induce strand breaks in plasmid DNA in the absence and presence of H2O2 was tested with the use of pBR322 plasmid Samples contained 15 lM DNA in 50 mM sodium phosphate buffer (pH 7.4) and ... Fragmentations occurred predominantly at glycosidic bonds between the rings and in the amide bond, linking the aglycon to ring B (see arrows at Fig and spectra at Fig 2) Single and double oxygen additions ... Also other aminoglycosides, as kanamycin A, neomycin B and a synthetic neomycin-neomycin dimer have been shown to induce fragmentation of tRNAPhe at Y37 and additionally at m7G46 in the variable...

Ngày tải lên: 21/02/2014, 01:21

... mds, and mss are the intercepts and slopes of the lower and upper baselines of the melting curves, respectively; T is the temperature of interest in Kelvin, a is the molar fraction of strands in ... loops in oligoribonucleotides Biochemistry 29, 278285 45 Evertsz, E.M., Rippe, K & Jovin, T.M (1994) Parallel-stranded duplex DNA containing blocks of trans purine-purine and purinepyrimidine base ... helix-to-coil melting is a non-two-state transition [27,30,32] and the difference in hydration between the duplex-stranded groups and single-stranded groups results in a heat capacity increase [2629,34,3742]...

Ngày tải lên: 22/02/2014, 07:20

... loops in various contexts involving three G-tetrads [27,31,42–47,55–60], as well as GTTA and GTTAG loops in Form involving two G-tetrads [47] Base pairing and stacking are generally observed in ... antiparallel-stranded core in the literature) (Fig 2C); and (d) two strands across one diagonal are oriented in one direction and the two remaining strands across the other diagonal in the opposite ... stacking 5′–5′ stacking 5′–3′ stacking 3′–3′ stacking 5′–3′ stacking 5′–3′ stacking 3′ 5′–5′ stacking 5′–5′ stacking 3′ 3′ 3′ Fig Models for arrangements G-quadruplexes in long human telomeric DNA...

Ngày tải lên: 06/03/2014, 09:22

... error processing but then freed again in the normal course of events 31 Leaking Containers in C++ In C++, standard containers that contain pointers not delete the objects to which the pointers refer ... myNewHandler(); void someFunc() { new_handler oldHandler = set_new_handler( myNewHandler ); // allocate some memory… // restore previous new handler set_new_handler( oldHandler ); } 24 Referencing ... Plugging Container Leaks It’s necessary to delete the container’s elements before the container is destroyed template inline void releaseItems( Container &c ) { typename Container::iterator...

Ngày tải lên: 08/03/2014, 11:20

... UCP3 protein and mRNA in the three muscles are comparable Effect of fasting on UCP3 protein expression We and others have shown that fasting induces upregulation of UCP3 mRNA expression in skeletal ... number of experiments in parentheses SS Fig Increase in the UCP3 signal as a function of increasing amounts of (A) human recombinant protein and (B) mouse recombinant protein Representative signals ... Muzzin, P & Giacobino, J.P (1998) The uncoupling proteins, a review Eur J Endocrinol 139, 1–9 Muzzin, P., Boss, O & Giacobino, J.P (1999) Uncoupling protein 3: its possible biological role and...

Ngày tải lên: 24/03/2014, 04:21

... while maintaining DNA in an intact, high-mol-wt form Thus, mixing of the STEL:phenol should be performed by gentle inversion, which minimizes shearing forces on the DNA The proteinaceous interface ... 32P-Labeling of DNA R Smith Introduction Random primed labeling of DNA has now almost superseded the method of nick translation of DNA Random primed labeling, based on the method of Feinberg and Vogelstein ... described by Feinberg and Vogelstein (2) in which a DNA fragment is denatured by heating in a boiling water bath Then, random sequence oligonucleotides are annealed to both strands Klenow fragment...

Ngày tải lên: 10/04/2014, 11:03

... molecular weight standards The arrow ( protein band ) indicates the protein band with a molecular weight of 35 kDa and the arrow ( Ara h4, and iso-Ara h3 [13] (Fig 4) Interestingly, all amino acid sequences ... runner runner runner China China China China China India USA USA USA USA USA USA [7-9], indicating that very low variation in protein profiles was detected in cultivated peanut using SDS-PAGE gel electrophoresis ... isoelectric focusing separating to left and the basic end to the right The arrow ( arrow ( ) indicates the protein band with a molecular weight of 35 kDa and the ) indicates the 27 kDa protein band (Fig...

Ngày tải lên: 12/08/2014, 05:20

... 3' and 5' inverted terminal repeats, also a 5' binding protein This protein can be involved in both replication and integration processes of these plasmids in the mitochondrial genomes [3,4] Interestingly, ... be circular or linear, and include complete protein coding genes, pseudogenes, non -protein coding genes and inverted repetitive elements The probable plasmid function in their fungal hosts is ... to bind terminal protein and DNA primer substrates J M Biol 1998, 278:741-755 Esteban JA, Salas M, Blanco L: Fidelity of Φ29 DNA Polymerase: Comparison Between Protein- Primed Initiation and DNA...

Ngày tải lên: 13/08/2014, 16:20

Ngày tải lên: 11/09/2015, 16:05

... is known to work by inhibiting thymidylate synthase (TS) and incorporating its higher nucleotide forms into RNA (FUTP) and DNA (FdUTP), leading to RNA and DNA damage (Diasio and Harris 1989) 5-FU ... (inhibitors of CDK4) and cip/kip family Four proteins belonging to the INK4 family are identified: p16INK4a, p15INK4b, p18INK4c and p19INK4d; and three proteins are identified belonging to the cip/kip ... fate determination and positioning in the intestinal epithelium 1.2 Intestinal immune system 1.2.1 Innate immunity A large number of indigenous microbial flora resides in the human intestinal tract,...

Ngày tải lên: 11/09/2015, 16:05

... PRAP1 and intestinal differentiation 3.1.1 PRAP1 is expressed in epithelial cells of the intestines The expression of PRAP1 in human intestinal tract was studied using immunohistochemistry Paraffin ... examined whether PRAP1 protein may also play a similar functional role as that of antibacterial peptides in the gastrointestinal tract PRAP1 being a secretory protein and expressed on the luminal ... WNT-TCF pathway inhibition The WNT-TCF signaling pathway plays a central role in controlling the switch between proliferation and differentiation in the intestinal epithelium (Pinto and Clevers 2005;...

Ngày tải lên: 11/09/2015, 16:05

... knockdown on cyclins and CDKs Mammalian cell cycle is controlled by a family of protein complexes The main cell cycle protein complexes involved in G1/S-phase are cyclin D/CDK4 and cyclin E/CDK complexes ... p53 in the induction of PRAP1 expression following exposure to DNA damaging agents Firstly, PRAP1 may be a direct or indirect transcriptional target of p53 Secondly, p53 which is a DNA binding protein, ... blot of PRAP1 and GAPDH in HCT 116 after transfection with PRAP1 specific siRNA (PRAP1 siRNA1 and 2) or PRAP1 siRNA specific control siRNA (control siRNA1 and 2) for 48 hours, and followed by...

Ngày tải lên: 11/09/2015, 16:05

... Identification of PRAP1 binding protein by MS-MS Representative figure showing the identification of PRAP1 binding proteins after performing MS-MS Figure 3.71 PRAP1 physically interacts with Hsp 70 ... of control siRNA and PRAP1 siRNA for 72 hours Anti-tubulin antibody was used to stain the microtubulin network (Green) and nuclear was counter stained with Hoechst (Blue) 143 binding partner ... stably expressing PRAP1 (PRAP1) and its empty vector control stable cell line (Vector), or after transfection of control siRNA and PRAP1 siRNA for 72 hours F-actin was stained using Palloidine conjugated...

Ngày tải lên: 11/09/2015, 16:05

... floating cells, we employed the annexin V-FITC staining assay Annexin V is a phospholipid binding protein that has a high binding affinity for phosphatidylserine (PS) that translocates from inner ... identified In the human gastrointestinal tract, antimicrobial peptides such as α-defensins (HD-5 in small intestine) and β-defensin (hBD-3 in stomach and colon) are the major players These defensins ... that expresses a doxycycline-inducible N-terminally truncated TCF-4 protein was used to mimic intestinal differentiation Upon induction by doxycycline, the dnTCF-4 protein effectively blocked the...

Ngày tải lên: 11/09/2015, 16:05

... 1.2.2.2 Mto1p and Mto2p 12 1.2.3 Organizing microtubule bundles 13 1.2.3.1 Minus-end-directed motor protein Klp2p 14 1.2.3.2 Bundling protein Ase1p 14 1.2.3.3 Model of maintaining ordered microtubules ... poles and NE division fails in Mia1p-overexpressing cells 80 3.2.3 Spindles in Mia1p-overexpressing cells are bipolar and require the microtubule-crosslinking protein, Ase1p, for their assembly ... Chapter III: Results 3.1 Mia1p in assembly and maintenance of persisent iMTOCs at nuclear envelope 44 3.1.1 Lack of microtubule binding protein Mia1p results in fewer interphase Microtubules 44 3.1.1.1...

Ngày tải lên: 12/09/2015, 08:19

... tips by depositing the kelch-repeat protein, Tea1p, at the cell tips There, Tea1p functions in polarity establishment by recruiting a complex, forminbinding protein Bud6p and the formin, For3p, to ... system identified a partner of D-TACC protein, another centrosomal protein -Minispindles (Msps), making a breakthrough in understanding normal function of TACC protein (Lee et al., 2001) Msps belongs ... thus contributing to maintenance of the antiparallel linear microtubule arrays in interphase cells 1.2.3.2 Bundling protein Ase1p Another protein which functions in organizing antiparallel microtubule...

Ngày tải lên: 12/09/2015, 08:19

... 10min at 4oC The pellet was air dried for 10min and resuspended in 40μl TE buffer containing RNAse and incubated at 37oC for 20min DNA was stored at -20oC 2.3 Construction of knock out mutants and ... MgSO4 and 1mM GTP] containing 5% milk and then with taxol-stabilized bovine brain 3X microtubules (50μg/ml) in the same buffer, followed by washing and immunoblotting withTAT1 anti-tubulin antibodies ... 10μl 20mg/ml proteinase K was added shortly buffer before use) and incubated at 65oC for 30min Samples were incubated on ice for 10min 200μl 5M Kac was added to the sample and mixed by inversion...

Ngày tải lên: 12/09/2015, 08:19

... coexpressing the NE marker protein, Uch2p-GFP, and the SPB protein, Sad1p-DSRed 84 3.2.3 Spindles in Mia1p-overexpressing cells are bipolar and require the microtubule-crosslinking protein, Ase1p, ... zstacks obtained by epifluorescence imaging 50 3.1.2.2 Microtubules remained attached to the SPB in alp14Δ cells Interestingly, I found that in the absence of Mia1p-interacting protein of the MAP215 ... the spindle midzone in 43% of Mia1p-overexpressing cells, and I found it localizing to kinetochores in 15% of cells (see Figure 3.2.3F) Another midzone marker is the microtubule-binding protein...

Ngày tải lên: 12/09/2015, 08:19