n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1

... changed to DMEM/F12 (Invitrogen, 10 565-042) supplemented with Non-essential amino acids (Invitrogen, 11 114 0050), N2 (Invitrogen, 17 502-048), heparin (Sigma, H 314 9) and FGF-2 (ProSpec, CYT-557 _10 µg) ... harvested using collagenase and seeded onto CoStar non-adherent plates (Corning, 34 71) to form embryoid bodies using 20% Knockout Serum (Invitrogen, A1099202) in Knockout DME...

Ngày tải lên: 11/09/2015, 10:06

... IPI0 022 0 327 Extracellular Matrix Ribosome-binding protein 66 022 IPI0 029 7646 138 923 IPI003049 62 129 297 IPI00013079 IPI00 328 113 IPI00 329 573, IPI009645 52 IPI00 022 200, IPI000 729 18, IPI0 022 0701 IPI00 022 418, ... Christopher Stephen Hughes and Prof Gilles Lajoie * Protein also found in GFR and standard Matrigel, based on work by Hughes et al 20 09 † Murine protein...

Ngày tải lên: 11/09/2015, 10:06

... matrices contains more Fn (by immunofluoresence comparison, section 3. 3.2), it would be expected that the 52 increased presence of Fn would aid in increasing integrin binding and hence cell binding This ... This contradiction could possibly be due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering with TGFB signaling for the hESCs...

Ngày tải lên: 11/09/2015, 10:06

... gastrulation in an anterior-posterior pattern Germ cells continue to maintain the expression of Oct -4 until the initiation of sexual differentiation [10] The under-expression of Oct -4 in ES cells ... their differentiation into trophoectoderm lineages [10], indicating the crucial role that Oct -4 plays in maintaining the pluripotent state of ES cells As Oct -4 is a transcription fa...

Ngày tải lên: 11/09/2015, 10:07

... and were transferred onto laminincoated plates and incubated in neural induction medium Under these conditions, hESCs were able to form extensions between embryoid bodies that were stained positive ... that maintain hESC pluripotency using dispase 3.7.1 Population doublings In section 3.4, 3 .5 and 3.6, all hESCs were passaged by enzymatic passaging using collagenase IV There is an...

Ngày tải lên: 11/09/2015, 10:07

... collagen deposition: Fab’ to the gelatin binding domain of fibronectin inhibits both fibronectin and collagen organization in fibroblast extracellular matrix J Cell Biol 92:485-492 (1982) 52 Velling ... amount of a beneficial substance might have adverse effects Indeed, it has been reported that MEFCM contains BMP-antagonists, noggin and gremlin, and hESCs can be maintained in...

Ngày tải lên: 11/09/2015, 10:07

... What are the implications on teaching, learning and – finite Clauses and Its Application in Vietnamese English translating non – finite clauses? Translation 1.5 ORGANIZATION OF THE STUDY 1.2 AIMS ... translated in Therefore, the results of the study of the ways of Vietnamese the form of a verb, a noun, an adverb and a finite clau...

Ngày tải lên: 26/11/2013, 13:19

... presence and importance of alumina in the natural aquatic environment and its growing application in drinking and wastewater purification, the knowledge of aluminas structure and possible interactions ... discussing the properties of alumina in aqueous solution Dissolution of alumina is an important process that influences soil solution chemistry and th...

Ngày tải lên: 15/03/2014, 23:22

... References Srinivas Bangalore and Owen Rambow 2000 Using TAG, a tree model, and a language model for generation In Proceedings of the Fifth International Workshop on Tree-Adjoining Grammars (TAG+) ... Franz Josef Och 2003 Minimum error rate training in statistical machine translation In Proceedings of the ACL, pages 160167 Kishore Papineni, Salim Roukos, Todd Ward, and W...

Ngày tải lên: 23/03/2014, 18:20

... Al concentrations in a solution of 0.025 M zinc nitrate [Zn(NO3)2·6H2O] and hexamethylenetetramine (HMT), we obtained ultrathin flake-like AZO nanostructures of high s and distinct morphology Moreover, ... 0.337, and a PCE of 1.04% With doping and mM Al concentrations, the J SC of devices increases to 10.26 and 11.08 mA cm -2 , and the PCE increases to 1.26% and 1....

Ngày tải lên: 20/06/2014, 22:20

... perspective into the characteristics of time-varying spectrum via studying the properties of the corresponding kernel However, to maintain physical meaningness in time-varying spectrum and coherence, ... normalization of the time-varying cross spectrum The time-varying coherence certainly inherits the characteristics of the time-varying spectra Therefore, the multisca...

Ngày tải lên: 21/06/2014, 16:20

... properties and advantages of watermarking in joint timefrequency domain In [15], watermark insertion and extraction are both done in time-frequency domain In the embedding process, watermark message ... provided in Section But first we will have a brief review on watermark applications in joint time-frequency domain TIME-FREQUENCY DIGITAL WATERMARKING Digital waterm...

Ngày tải lên: 22/06/2014, 23:20

... of N data points to be clustered The local maximum of a data point i is the data point whose magnitude is the maximum among all the data points within a certain distance from the data point i ... the clustering results of different methods The letters L, H, K, and S stand for the LMC method, the hierarchic clustering method, the K-mean clustering...

Ngày tải lên: 23/06/2014, 01:20

... success in taming complexity Sort Label Integrate Prioritize An effective scheme for organization achieves definitive success in taming complexity Sort Label Integrate Prioritize Done! Savings in ... is about subtracting the obvious and adding the meaningful If you that Want to learn the remaining secrets of simplicity? Click here to get the book at Amazon I’ve read it and think it...

Ngày tải lên: 03/07/2014, 09:47