Metabolomics study of CHO cell cultures

... scans Appendix E List of putative extracellular metabolites that displayed strong correlation (R2>0.8) with intracellular caspase activities 141 Metabolomics study of CHO cell cultures SUMMARY Chinese ... Assessment of CHO mAb physiology 47 3.4.1 Assessment of enzymatic bottleneck at MDH II 47 3.4.2 Assessment of apoptosis induction 48 CHAPTER Development of strategy f...

Ngày tải lên: 11/09/2015, 10:01

... zone clearly separated into sub-zones where cell division, cell enlargement and secondary wall formation were taking place (figure 1a) PATAg staining revealed the absence of secondary walls close ... secondary walls (S) of differentiating fibres in a wild type (a) and a transgenic (b) tree Absorption at about 272 nm indicating lignification of middle lamella regions...

Ngày tải lên: 08/08/2014, 14:20

... nature of goblet cell carcinoids, the location of hormones and the phenotype of endocrine cells Another useful method for simultaneous revealing of mucin secretion and endocrine cell component of the ... with the hypothesis of the dual entodermal and neuroendocrine origin of goblet cell carcinoid [1] The ultrastructural investigation showed that...

Ngày tải lên: 09/08/2014, 07:21

... Figure Metabolite linkage map representing primary and secondary plant metabolism in opium poppy Metabolite linkage map representing primary and secondary plant metabolism in opium poppy The circles ... development of the still untapped potential of 1H NMR metabolomics and targeted profiling The metabolic demands of the defence response in elicit...

Ngày tải lên: 12/08/2014, 05:20

... Batch cultures 49 3.1.4 Feeding of batch cultures 49 3.1.5 Rapamycin treatment of batch cultures 50 3.1.6 Determination of cell viability 50 3.1.7 Determination of ... Background Chinese hamster ovary (CHO) cells are one of the most commonly used mammalian host cell lines by biopharmaceutical industries for the production of recombinant proteins of therapeutic ....

Ngày tải lên: 08/09/2015, 19:49

... operating system Figure Computational mesh of a tubular-shaped ambient air-breathing PEM micro fuel cell (quadratic) The values of the electrochemical transport parameters and the material properties ... fuel cell performance 2.1 Computational domain A schematic description of a tubular-shaped air-breathing PEM micro fuel cell is shown in Figure 1a...

Ngày tải lên: 05/09/2013, 14:58

... converted into Man4GlcNAc2, in contrast with the small amount of Man6GlcNAc2 originating from the Man9GlcNAc2 species ERAD of glycoproteins bearing Man5 and Man9 populations in MI8-5 cells The N-glycosylation ... ratio Glc 1Man5GlcNAc2/ Glc 1Man9GlcNAc2 obtained with the glycoprotein pattern This indicates that, when glycoproteins bearing Man9 and Man5 are s...

Ngày tải lên: 16/03/2014, 16:20

... studied in wheat immature embryos The role of cell permeabilizing agent in enhancing the uptake of CPPs alone and cargo complex in the immature embryos was evaluated Cellular uptake of CPPs is ... the combined use of cell permeabilizing agent and cell- penetrating peptides results in the delivery of such large sized cargoes in plant cells invol...

Ngày tải lên: 23/03/2014, 07:20

... growth and secreted AP activity of tomato suspension cells Tomato cells cultured for days in the absence of exogenous Pi had only approximately 40% of the fresh weight of the 8-day-old +Pi cells ... phosphoenolpruvate-hydrolyzing specific activity of 222 UÆmg)1 and a recovery of approximately 3%, whereas SAP2 was purified 50-fold to a final phosphoenolpruvate-hydrolyzing ac...

Ngày tải lên: 23/03/2014, 21:20

... vascular cell types obtained from human umbilical cords (UCs) Cell proliferation kinetics of vascular cell types obtained from human umbilical cords (UCs) Human umbilical artery and vein endothelial ... of exponentially growing and confluent human umbilical artery endothelial cell cultures Proliferation fraction of exponentially growing and conflu...

Ngày tải lên: 18/06/2014, 15:20

... Ribas et al.: Multicenter phase II study of matured dendritic cells pulsed with melanoma cell line lysates in patients with advanced melanoma Journal of Translational Medicine 2010 8:89 Submit your ... Dacarbazine (DTIC) versus vaccination with autologous peptide -pulsed dendritic cells (DC) in firstline treatment of patients with metastati...

Ngày tải lên: 18/06/2014, 16:20

... least for the CD4+ T cell subset, the only one that could be tested Evaluation of cytokine production Next, we investigated the production of cytokines by cultures in response to different stimuli, ... outgrowth assays that evaluate the ability of a fixed input of T cells to inhibit long-term growth of different numbers of target cells, without the addition of cytokines...

Ngày tải lên: 18/06/2014, 16:20

... concentrations during defence response in Norway spruce embryogenic cell suspension cultures induced by intracellular protein and wall preparations from Sirococcus strobilinus mycelia MATERIAL AND METHODS ... ester-bound (SWE) and soluble glycoside-bound (SG) phenolic acids in control cells of embryogenic cultures of Norway spruce (C) and in the...

Ngày tải lên: 07/08/2014, 10:21

... Patients/Eligibility This study was available to patients with locally advanced NSCLC who required definitive full dose radiotherapy as part of their treatment plan This included stage IIIA, IIIB ... effective radiosensitization regimen for definitive therapy of locally advanced nonoperative NSCLC Despite excellent in field control, progression of disease outside the radiati...

Ngày tải lên: 09/08/2014, 09:20

... between blind- and non-blind-performed investigations a-Amylase treatment in human mammary epithelial cells The effect of a-amylase in mammary cells of human origin was studied in primary HBCEC (mammary ... antiproliferative effects in primary rat mammary epithelial cells and human breast tumor cells On the one hand the effects on hea...

Ngày tải lên: 10/08/2014, 10:21