plant cell cultures as producers of secondary compounds

... milk sample was classified as positive if at least one colony-forming unit (CFU) of S aureus was isolated For other agents, the presence of at least three CFU was needed for positive classification ... specificity of CMT as an indicator of IMI, for different CMT cut-offs Figure shows the sensitivity and specificity for SCC measured by DCC (SCC-DCC) as an indicator of IMI Page of Table Number of samples ... [13] was used to optimize the cut-off of the sensitivity and specificity test Results Bacteriology and somatic cell count Intramammary infection, defined as growth of udder pathogens, was found...

Ngày tải lên: 12/08/2014, 18:22

... IX List of Tables XI List of Figures XII List of Schemes XIV List of Abbreviations List of Publications XX XXII I Introduction: chiral organofluorine compounds I-1.Importance of organofluorine ... application of 2-fluoro-1,3-dicarbonyl compounds as Michael addition donors, which results in enantioselective preparation of compounds being direct precursors of fluoro-isosteres of glycerine ... conclusion, as well as preliminary results and future perspective As an implication of studies towards decarboxylation/asymmetric protonation, decarboxylative addition of fluoromalonate halfesters was...

Ngày tải lên: 09/09/2015, 11:25

... truncatula cell cultures using gas chromatographymass spectrometry (GC-MS) [9], carotenoid profiling using matrix-assisted laser desorption ionization time-offlight mass spectrometry (MALDI-TOF) [10], ... understanding of cellular responses to environmental change Reports of metabolite profiling in the context of defence-related plant secondary metabolism, although rare, include the analysis of elicitor-treated ... and/or storage of nitrogen In elicitor-treated opium poppy cells, asparagine increased in abundance later than most amino acids The concentration of asparagine also increased in Pseudomonas syringae-infected...

Ngày tải lên: 12/08/2014, 05:20

... (4) establishment of cell suspension cultures; (5) analysis of metabolite levels in cell suspension cultures; (6) selection of cell lines based on single cells; (7) analysis of culture stability; ... functional status of cells is now routinely performed for plant cell cultures in order to permit accurate assessment of growth and metabolite production rates The availability of plant cells for quantitative ... 2.1 Plant Cell Factories as a Source of High-Value Metabolites The presence of valuable metabolites in plants has stimulated interest on the part of industry in the fields of pharmaceuticals (as...

Ngày tải lên: 25/10/2013, 05:20

... II All assays were performed at least in duplicate NADH oxidase in a desalted, cell- free extract of Escherichia coli DH5a was assayed continuously as the decrease in A340 in the presence of NADH ... the reaction tube was heated at 95 °C for The AMP generated was determined as follows in stage II of the assay Fifty microlitres of the AMP-containing sample was added to 950 lL of reaction mixture ... catalyse the production of AMP from NADH, ATP and FAD (Table 3) Ninety percent of the amount of NADH used in our standard NR assay, and 60% of the amount of ATP in a GS assay, was converted into AMP...

Ngày tải lên: 08/03/2014, 08:20

... 26S proteasome 19S cap Ubiquitin 4315 Quinone reductase as regulator of the proteasome S Sollner and P Macheroux The impact of the 20S proteasome on QR activity raised the question of whether ... latter protein gives rise to increased levels of intracellular ROS, thereby promoting apoptosis of damaged cells [46,47] This generation of ROS increases expression of NQO1 [48] which then further ... mammalian to yeast cells, as well as regulatory proteins such as ODC, suggest that short-lived proteins are intrinsically prone to degradation by the 20S proteasome The association of a QR (NQO1,...

Ngày tải lên: 16/03/2014, 02:20

... concentration of enzyme assayed One unit (U) of activity is defined as the amount of AP resulting in the hydrolysis of lmol of substrate per at 25 °C Phosphatase assay A For routine measurements of AP ... gel of AP from the media of tomato suspension cells Lanes and 2, respectively, contain lg of culture media proteins from 8-day-old +Pi and –Pi cells The gel was incubated in a casein/EDTA wash ... absence of exogenous Pi had only approximately 40% of the fresh weight of the 8-day-old +Pi cells (approximately 63 and 25 g of cells were obtained per 500 mL culture of 8-day-old +Pi and –Pi cells,...

Ngày tải lên: 23/03/2014, 21:20

... Ac-LDL as described [16] Cellular senescence Cells were seeded as above, and the percentage of senescent cells was determined as follows Cell culture media was removed from the well and mL of DPBS ... contamination of SMCs in EC cultures was observed, and a low average level (

Ngày tải lên: 18/06/2014, 15:20

... T cells were adjusted to 1.5 × 106 cells/ml and CD8+ T cells to × 106 cells/ml Cytotoxicity assays Cytotoxic activity of CD4+ and CD8+ T cells was determined in a standard 4-h 51Cr release assay, ... Sigma-Aldrich) Baseline cytokine production was determined in supernatants from unstimulated T cells, or LCL only Cytokine secretion was measured after 5h-incubation Outgrowth assay Outgrowth assay was carried ... perforin-based pathway, and BFA and anti-FasL mAb that interfere with Fas/FasL-based pathway Figure shows mean values from independent experiments carried out for each donor cell line of cells required...

Ngày tải lên: 18/06/2014, 16:20

... level of SG increased by about 40% after h After 12  h of treatment with PF the amount of SG was still at its 8-h level, but levels of the other forms of phenolic acids increased; levels of F ... albumin as a standard The final protein content of the intracellular fraction in 100 ml of liquid medium was mg Preparation of mycelial wall fraction The mycelial cell wall fraction (WF) was prepared ... establishment of suspension cultures, approximately g fresh weight of embryogenic tissue were inoculated to 100 ml of liquid medium of the same composition as mentioned above in 250 ml Erlenmeyer flasks...

Ngày tải lên: 07/08/2014, 10:21

... duration of cell cycling as well as expansion of developing xylem cells by positional signalling Therefore, auxin regulates the width of the developmental sub-zones cell division, expansion, and secondary ... initiation of cambial activity after winter dormancy has led to the assumption that in 35S-rolC transgenic aspen the basipetal transport of auxin, the sensitivity of the cambium to auxin or the ratio of ... differentiation of xylem cells, though, evidence exists that auxin and other phytohormones as well as second messengers such as calcium moderate the control of cambial activity and vascular cell elongation...

Ngày tải lên: 08/08/2014, 14:20

... a-amylase a-Amylase (50 U/ml) decreased the number of cells only in P1-F344-cells, but not in P2-F344- and P2-Lewis-cells Proportion of SA-b-gal-positive cells did not correlate with cell number, as ... the amount of dead cells was not influenced by a-amylase (Figure 2c) Thus, prolonged a-amylase treatment reduced the number of non-viable cells in F344 cells, but not in Lewis cells Based on these ... breast tumor, a-amylase caused a significant decrease in number of cells after 1.25 and 125 U/ml a-amylase for days (Figure 4a) The portion of SA-b-gal-positive cells was significantly increased...

Ngày tải lên: 10/08/2014, 10:21

... Cytotoxic effect of Cd2+ ion As cytotoxic effects of cadmium-based QDs are often attributed to Cd2+ ion release, the cytotoxicity of Cd2+ ions to Caco-2 cells was first investigated Cells were incubated ... conducting the MTT assay, medium was changed but cells were not rinsed, so assay results are indicative of mitochondrial activity of firmly as well as loosely attached cells The number of attached live ... decreased as the concentration of QDs increased Cell groups treated with lower concentrations of QDs had a higher quantity of attached dead cells, because the total amount of attached cells was much...

Ngày tải lên: 11/08/2014, 00:22

... of bullet around the hip joint He was managed conservatively at that time Now he was complaining of hip pain for the last two years which had progressively increased significantly over the last ... arthroplasty Page of (page number not for citation purposes) Journal of Medical Case Reports 2007, 1:171 http://www.jmedicalcasereports.com/content/1/1/171 reviewed the case MAW reviewed the case, helped ... microorganism There was extensive synovitis inside the degenerated acetabulum The loose bullet fragment was removed easily and an un-cemented total hip arthroplasty (Protek, Mathys Medical) was performed...

Ngày tải lên: 11/08/2014, 10:23

... binding, has no kinase activity [13] To examine whether purified GST-AtHaspin has kinase activity, an in vitro kinase assay was performed using purified GST-AtHaspin and GST-AtHaspin KD (kinase dead) ... sequence of AtHaspin cDNA showed 38% similarity with human Haspin in the kinase domain Recently, the crystal structure of the kinase domain of human Haspin was solved [28,29] Although AtHaspin showed ... (http://psort.nibb.ac.jp/form.html) Gray box indicates kinase domain (C) Multiple alignment of kinase domain of AtHaspin, human Haspin, and fission yeast Haspin Missing residues are shown as dashes, identical amino acids...

Ngày tải lên: 11/08/2014, 11:22

... Journal of Medical Case Reports 2009, 3:9331 http://www.jmedicalcasereports.com/content/3/1/9331 Histologically, the growth of fibroblastic cells is followed by a reactive proliferation of histiocytic ... below the tumor was also removed However, the complete cartilage of the joint was not damaged Case presentation Case report A 30-year-old Caucasian man suffered from a distortion of his right ankle ... spindle cells and some were multinucleated giant cells (Figure 2B) The diagnosis of localized tenosynovial giant cell tumor of the tendon sheath was confirmed on histopathology Results of special...

Ngày tải lên: 11/08/2014, 14:21

... trees As such fig cultivation in its native range fits the global picture of frequent hybridization of cultivated plants with their wild relative [36] However the case of fig is particular as new ... corresponding phenogram was drawn based on the software Treeview 6.1 Discriminating power, D, was calculated for each SSR locus as Dj = ∑pi [(Npi-1)/N-1)] [41] where pi was the frequency of the i-th molecular ... this article as: Achtak et al.: Traditional agroecosystems as conservatories and incubators of cultivated plant varietal diversity: the case of fig (Ficus carica L.) in Morocco BMC Plant Biology...

Ngày tải lên: 12/08/2014, 03:21

... expression and translocation of the hRzs to the cytoplasm, and a stretch of 6 0As is attached to the 3' end of the hRz sequence for recruitment of RNA helicase Page of 17 (page number not for citation ... contributions PN was responsible for design and completion of the bulk of the research, as well as for data analysis and initial writing of this report JK was responsible for identification of the Ae ... A B Figure RT-PCR 2of total RNA extracted from hRz-C6/36 cells RT-PCR of total RNA extracted from hRz-C6/36 cells (A) hRz expression in the cells was detected by the presence of an hRz-specific...

Ngày tải lên: 12/08/2014, 04:21

... with At catalase After transient expression, both YFP::catalase2 and DsRed2::catalase2 constructs did label peroxisomes in onion cells (Figure 1A) as well as in tobacco leaves, as expected (Figure ... YFP::catalase and DsRed2::catalase label peroxisomes A) Transient expression of YFP::catalase (yellow) in onion cells 24 h after bombardment Maximum projection (cell depth = 92 µm) of 50 z-stack ... expression of YFP::catalase (yellow) in tobacco leaves 48 h after Agrobacterium infiltration Autofluorescence of chloroplasts is pseudo-colored in red C) Transient expression of DsRed2::catalase (red)...

Ngày tải lên: 12/08/2014, 05:20