Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 8
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 2
... intracellular Ca2+-release To investigate the participation of mitochondria- specific CD38 in mediating Ca2+ release from ryanodine-sensitive Ca2+ stores, an in vitro assay was set up using intact mitochondria ... activity and specific topology of CD38 and its role in Ca2+-release assay observed from the data, this suggest that mitochondrial CD38 plays a role in cADPR synthesis and may participate in a ... with a mitochondrial targeting signal Further investigation of subcellular localization of expressed CD38 in mitochondria in parallel with the ADPribosyl cyclase assay had demonstrated localization...
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Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 3
... that CD38 staining is mainly associated on the outer membrane of mitochondria It is interesting to note that instead of a uniform distribution of the CD38 staining around the outer mitochondrial ... Expressed in Mitochondia from Murine Brain 4.2.4 Determination of the enzymatic activities of brain mitochondrial CD38 4.2.4.1 Determination of ADP-ribosyl cyclase activity of mitochondrial CD38 The ... intermembrane space and the inner mitochondrial membrane was analyzed to determine the integrity of the mitochondria OPA1, a protein of the intermembrane space, was protease resistant in mitochondria but...
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Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 4
... antibody-sc-7 049 A, B: intense CD38 immunostaining labeled the outer membrane of mitochondria (arrow), ER as well as plasmalemma (arrow head) Note the immunostained ER and mitochondria were in close ... bar: 1, 2µm 186 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain A B Figure 4. 14 CD38 immunoreactivity in the mouse cerebellum, labeled with goat polyclonal CD38 antibody-sc-7 049 ... immunopositive mitochondria located in Basket cell The enlargements of the encircled area of the basketcell are shown in the following page Blood vessel was heavily stained with CD38 antibody (arrow) Scale...
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Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 8
... Isolation and characterization of intact mitochondria from neonatal rat brain Brain Res Protocol 8: 176- 183 Nishina H, Inageda K, Takahashi K, Hoshino S, Ikeda K and Katada T (1994) Cell surface antigen ... organelles capable of generating and conveying electrical and calcium signals Cell 89 : 1145–1153, 1997 Ikehata F, Satoh J, Nata K, Tohgo A, Nakazawa T, Kato I, Kobayashi S, Akiyama T, Takasawa ... the functional role (s) of brain mitochondrial CD 38 A study of CD38KO mice (Jin et al., 2007) was conducted and showed that transmembrane CD 38 has an essential role in regulating the secretion of...
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Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 5
... immunostaing is restricted to surface of mitochondria (arrow) Scale bar: 0 .5 m 189 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain Gd Figure 4.17 CD38 immunoreactivity in ... of staining with CD38 involving the surface of the outer mitochondrial membrane facing the cytosolic side In contrast to the immunogold labeling of CD38 on the outer mitochondria membrane, all ... localization in mitochondria isolated from mouse brains, labeled with goat polyclonal CD38 antibody-sc-7049 AB, representative images of mitochondria isolated from mice brain stained with antiCD38 antibodies...
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Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria
... mobilization agents such as ix cADPR, ADPR and NAADP and thus may have a critical role in intracellular Ca2+ signalling x ABBREVIATIONS ADPR adenosine diphosphate ribose ATRA all-trans-retinoic acid BSA ... NUDT9-H domain (Kuhn et al., 2004; Perraud et al., 2005) These data revealed that ADPR and NAD+ act as intracellular messengers and may play an important role in Ca2+ influx by activating TRPM2 in immunocytes ... to human CD38 while a faint CD38 hybridizing band was detected in tomato DNA (refer to review by Ferrero and Malavasi, 1999) Analysis of the public sequence databases showed that proteins sharing...
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Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 6
... the surface of mitochondria 1 96 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain A B Figure 4.20 Backscatter electron micrographs of mitochondrial fractions, labeled ... varying size of mitochondria with dense cristae with varying shapes of round, tubular to spherical (Figure 4.20B) 195 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain Mitochondrial ... Scale bar: 1µm 197 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain A B Figure 4.21 Backscatter electron imaging (BEI) of mitochondrial fractions, labeled with goat...
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Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 7
... important to examine its precise localization on the organelle Mitochondria are compartmentalized into matrix, inner mitochondria membrane, intermembrane space and outer mitochondrial membrane, as discussed ... tend to argue against any artifactual rearrangement of the mitochondrial membranes during experimental manipulation All of the above considerations strongly suggest that the DAB stain accurately ... CD38 in the recent years The possibility that expressed intracellular CD38 has an important role in intracellular signaling and yet maintaining its membrane bound locality is important and cannot...
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Báo cáo khoa học: Subcellular compartmentalization of FADD as a new level of regulation in death receptor signaling pdf
... involving active caspase-8 Discussion FADD is an essential adaptor protein in the CD95mediated apoptotic signaling cascade that couples activated receptors with the activation of initiator caspase-8 ... triggering induces membrane proximal signals to induce nuclear export of FADD that are independent of CD95 internalization and ‘classic’ apoptotic signaling events, such as DISC formation and caspase-8 ... signals at the plasma membrane that lead to the translocation of nuclear FADD to the cytoplasm In a process that depends on a positive feedback loop involving caspase8 activation, cytoplasmic FADD...
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Báo cáo y học: " HIV infection of non-dividing cells: a divisive problem" ppt
... protein and HIV- 1 IN appear to behave in a remarkably similar way [25,140] Adenoviral pVII and HIV- 1 Rev and IN are all small, basic nucleic acids-binding proteins and may share similar nuclear import ... results cannot be accurately measured Alternatively, a difference in the rate rather than absolute amount of HIV- 1 DNA nuclear import may not be detectable in spreading assays due to the highly asynchronous ... reasons for this discrepancy are not completely clear One simple explanation would be that virus growth in spreading assays is generally measured by p24gag ELISA or by enzymatic RT assays and...
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A study to investigate the involvement of nadph oxidase 5 (NOX5) in resveratrol (RSV) induced reactive oxygen species (ROS) production in u937 cells
... esophageal cells resulting in esophageal adenocarcinoma (EA) Acid reflux damage is a major factor contributing to the transformation of EA NOX5-S was found to be the major NOX5 isoform present in ... is able to activate/ modulate signal transduction pathways such as the MAP kinase signaling pathways, JAK/STAT signaling pathways, PI3K/Akt pathway and cAMP/cGMP signaling pathway (Holme and ... death Platelet –activating factor (PAF), a pro-inflammatory, mediator was shown to regulate acid -induced NOX-5S expression (Si et al., 2008) NOX5-S increases upon PAF treatment and STAT5 was...
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báo cáo khoa học: "A Study to investigate the role of p27 and Cyclin E immunoexpression as a prognostic factor in early breast carcinoma" pptx
... post-transcriptional level [20] The aim of this study was to investigate the role of p27 and cyclin E immunoexpression as a prognostic factor in early breast carcinoma Methods A database of all wide local excisions ... there were racial differences in breast carcinoma When disease stage and age at diagnosis were adjusted for, it was shown that African American (AA) women have increased odds of having features ... justified The inverse relationship of cyclin E and p27 has been highlighted in the literature [13] However, in this study the association of p27 and cyclin E in all invasive carcinomas and in the...
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báo cáo khoa học: " Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing" ppsx
... as: Xin et al.: Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing ... response to biotic and/ or abiotic stresses in wheat Results Identification of powdery mildew infection and heat stress responsive long npcRNA candidates in wheat In our previous study, a total of 9744 ... microRNAs in wheat was regulated by powdery mildew infection and heat stress, which stimulated us to explore whether long npcRNA was also responsive to powdery mildew infection and/ or heat stress...
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Báo cáo y học: "Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection" pptx
... mesenchymal stem cells; HD: hematopoietic differentiated cells; AIDS: acquired immune deficiency syndrome; HAART: highly active anti-retroviral therapy; HPCs: hematopoietic progenitor cells; ... established as to how these mesenchyme derived cells become susceptible to HIV-1 and whether their HIV-1 production rates are comparable to that observed in HIV-1 infected lymphoid or myeloid cells Importantly, ... HD-HIV cells versus un-infected HD cells The gene profile of HD-HIV cells was then compared to HUT78-HIV cells The analysis showed that HIV-1 infection altered gene expression within HD cells...
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Báo cáo khoa học: " Development of liver dysfunction under artificial nutrition: a reason to modify nutrition therapy in the intensive care unit" docx
... component of modern medical therapy In many cases the primary disease is the decisive cause of the development of liver dysfunction; intravenous or even enteral nutritional support may be an aggravating ... Parenteral nutritionassociated hepatotoxicity Nutr Clin Pract 2004, 19:274-283 Kubota A, Yonekura T, Hoki M, Oyanagi H, Kawahara H, Yagi M, Imura K, Iiboshi Y, Wasa K, Kamata S, et al.: Total ... Critical Care Vol 11 No Stehle appearance any type of liver dysfunction in their mixed patient population It can be speculated that a delayed nutritional support of (already malnourished) ICU patients...
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Từ khóa: by the end of the written test t will be able to check knowledge s students from unit 8 to unit 9 through some exercisessite directed mutagenesis as a tool to characterize specificity in thiol based redox interactionexample of an approved consent form for a studyuse of non hematopoietic stem cells of fetal origin from cord blood umbilical cord and placenta in regeneration medicineactivation of t lymphocytes and other signaling events in hematopoietic cells5 possible clinical applications of non hematopoietic fetal stem cellscounta function returns the number of non blank cells in a cell range các counta trả về số lượng các tế bào không trống trong một loạt các tế bàofunction of 4e in non hepatoma cellsdivision of labour in cellsprocess of photosynthesis in non green plantsdivision of labour in animal cellsdivision of labor in eukaryotic cellsadvantages of division of labour in cellsimportance of division of labour in cellsdefine division of labour in cellsBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếChuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)Chiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015