function of 4e in non hepatoma cells

... activity increases in response to incubation with retinoic acid results in a manifold increase of intracellular cADPR content in cultured HL-60 cells (Takahashi et al., 1995) In sea urchin eggs ... of the cADPR-mediated intracellular Ca2+ signaling in T-lymphocytes and observed no increase of intracellular cADPR when the intact cells were incubated with NAD+ Therefore, the feasibility of ... distinguished from non- transfected cells based on their high immnunofluorescence intensity and most prominently, their organelle’s specific staining pattern (Figure 3.9 A-C) Immunostaining of...

Ngày tải lên: 11/09/2015, 09:07

... Purkinje cells, the subcellular localization of CD38 in cerebellar neurons was noted in the perikarya, axon terminals and dendrites, in order of decreasing intensity Although the labeling of the ... cADPR, indicating the presence of the complex receptor machinery that is responsible for the opening of the Ca2+ channels in these stores In mouse brain, CD38 was found in both neurons and glial cells, ... observed in intact cells at cytosolic Ca2+ concentration ([Ca2+]c) as low as 150-300nM (Pitter et al., 2002) In the event of high local Ca2+ domains of tens of micromolar in the vicinity of voltage-operated...

Ngày tải lên: 11/09/2015, 09:08

... Immunoreactivity was most intense with mitochondria Varying degree in CD38 immunostaing of mitochondria observed in different compartment Lower panel: immunopositive mitochondria located in Basket cell ... Expressed in Mitochondia from Murine Brain A B Figure 4.14 CD38 immunoreactivity in the mouse cerebellum, labeled with goat polyclonal CD38 antibody-sc-7049 A, B: intense CD38 immunostaining labeled ... enlargements of the encircled area of the basketcell are shown in the following page Blood vessel was heavily stained with CD38 antibody (arrow) Scale bar: 1, 2µm 186 Chapter Characterization of CD38...

Ngày tải lên: 11/09/2015, 09:08

... unique in the sense that in addition to extracellular signaling, it is also involved in intracellular signaling This is particularly interesting in which mitochondria are known as central to intracellular ... CD8+CD38+CD45RO+ T cells predict the decline of CD4+ T cells in HIV-1-infected patients AIDS 10:827-834 Bolen JB (1993) Nonreceptor tyrosine protein kinases Oncogene 8:2025-2031 Boquist L (1984) Inhibition ... Ferrarini M (1995) Transfection of the cmyc oncogene into normal Epstein-Barr virus-harboring B cells results in new phenotypic and functional features resembling those of Burkitt lymphoma cells...

Ngày tải lên: 11/09/2015, 09:08

... micrographs obtained showed the pattern of internal staining, as opposed to the pattern of staining with CD38 involving the surface of the outer mitochondrial membrane facing the cytosolic side In contrast ... noise level of the immunogold staining in the electron micrographs was low in all the sections observed 191 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain In comparison ... the granular layer (A), in particular association with mitochondria in varying degree of CD38 staining intensity (B) Notice the the immunostaing is restricted to surface of mitochondria (arrow)...

Ngày tải lên: 11/09/2015, 09:08

... analog of NAD+ with guanine substituting for the adenine group, to produce cGDPR, a fluorescent analogue of cADPR and the site of cyclization is N7 of the guanine ring instead of N1 of the adenine, ... micrometer Zn2+ zinc xiii Chapter Introduction CHAPTER INTRODUCTION 1.1 General Introduction of CD38 1.1.1 The Beginning CD38 was originally defined by Reinherz et al (1980) in their pioneering work ... CD38 reactivity in neural cells of the human brain (Mizuguchi et al., 1995) is reinforced by the finding of CD38 mRNA in the brain (Takasawa et al., 1993a) Interestingly, no human fetal organ...

Ngày tải lên: 11/09/2015, 09:08

... Characterization of CD38 Expressed in Mitochondia from Murine Brain 4.2.5 Determination of the localization of CD38 on mitochondria using Scanning Electron Microscopy (SEM) 4.2.5.1 Determination of the ... clearly distinguishable in the matrix display tubular cristae with lumen in continuity with space between the inner and outer mitochondrial membrane The predominant morphology was varying size of mitochondria ... allow the examination of the localization and quantification of molecule on the surface of mitochondria 196 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain A B Figure...

Ngày tải lên: 11/09/2015, 09:09

... observation is interesting, in account of accumulative research in the connectivity and functional homogeneity of mitochondria in cells; recent publications showed the 208 Chapter Characterization of CD38 ... Expressed in Mitochondia from Murine Brain dynamic nature of mitochondria within individual cells, including neurons, which could be morphologically heterogeneous and unconnected thus allowing them ... extracellular domain (carboxyl-terminal domain) containing the enzymatic site facing the extracellular region would suggest luminal localization of the CD38 enzymatic site for intracellular CD38 Indeed,...

Ngày tải lên: 11/09/2015, 09:09

... antiviral cytokine response of infected cells to a large extent Interestingly, however, induction of the chemokines IP-10 and IL-8 escaped suppression by SARS-CoV in Caco-2 cells, but not in HEK 293s ... dissemination of SARS-CoV By slowing down expression of IFNs and their antiviral genes in the infected tissue cells, the virus buys time during the initial, critical phase of infection in order ... was detected in HEK 293 cells, and none for IFN-β or the IFN-λs in either cell line All preparations contained similar amounts of input RNA, since the γ-actin control mRNA was present in equal amounts...

Ngày tải lên: 20/06/2014, 01:20

... Specific protein complexes such as protection of telomeres-1 (POT1), telomeric repeat-binding factor-1 (TRF1), TRF2, TRF1-interacting protein-1 (TIN1), TIN2, TINF2-interacting protein (TPP1) and ... since it is detectable in 85 to 90 % of tumours and not in normal somatic cells, with the exception of germline cells and the proliferating cells of renewal tissues (e.g bone marrow cells, intestinal ... checkpoint function in breast cancer cells 41 3.1.3 Changes in cell cycle protein expressions in TQ-treated breast cancer cells 44 3.2 DNA damaging effects of TQ in normal and breast cancer cells...

Ngày tải lên: 30/09/2015, 13:41

... specification in column (4) by adding a dummy variable for knowing English very well There are a number of interesting findings when we add knowledge of English to the equation First, we find a large ... suggest that the choice is either to invest in language skills or in other forms of education In fact, one of the key findings in this paper is that knowledge of the foreign language, English, as ... Our first innovation in the paper is to include English language knowledge in the regression As with Hebrew, we begin by including dummy variables for knowing English well and very well In contrast...

Ngày tải lên: 24/01/2014, 04:20

... neuraminidase activity should enhance PNA staining of the control cells, with a minor in uence on the p16INK4a-expressing cells and on DBA staining The importance of this aspect has been pointed ... domain As in the binding studies with plant lectins, we routinely performed experiments to assess concentration dependence and inhibition of galectin binding by haptenic sugar Profiling of galectin ... New function of p16INK4a 3239 New function of p16INK4a ´ S Andre et al when compared with the relatively increased lectin reactivity on the cell surface for p16INK4a-expressing cells, indicating...

Ngày tải lên: 16/03/2014, 10:20

... structure of a network, including the devices and media that are necessary for successful communications Explain the function of protocols in network communications Explain the advantages of using ... addresses, Original source address of host Final destination address of host Used by routers in selecting the best path 35 Interaction of Protocols frame Network access protocols (Data link and Physical ... stack) is a particular software implementation of a computer networking protocol suite The terms are often used interchangeably Strictly speaking, the suite is the definition of the protocols, and...

Ngày tải lên: 01/04/2014, 12:20

... Taiwan 3Institute of Pharmacology, National YangMing University, Taipei, Taiwan 4Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan 5Institute of Biomedical ... alkylating agents by linking the alkylating pharmacophore to the DNA-affinity molecules (e.g., DNA intercalating agents, DNA minor groove binder) [7,8] In most cases, the DNA-directed alkylating ... days of incubation, colonies were fixed with methanol and stained with Giemsa The number of colonies containing at least 50 cells was determined, and the plating efficiency (PE) and surviving...

Ngày tải lên: 09/08/2014, 09:20

... staining of STC-1 in tumor tissues of nude mice STC-1 was detected on the membrane of tumor cells (D) Immunohistochemical staining of PCNA in tumor tissues of nude mice PCNA was detected in the ... angiogenesis of BGC cells in nude mice (A) Western blotting analysis of the expression of STC-1 in BGC after stable transfection (B) Mean volumes of the tumor in each group were calculated Cultured BGC cells ... individual mice and three independent in vivo experiments (F) Hematoxylin and eosin stained sections of the Matrigel plugs (E, endothelial-like cells; T, tumor cells; S, surrounding tissues; V, microvessels)...

Ngày tải lên: 10/08/2014, 05:21

... rapamycin will result in some beneficial modulation of the cancer killing properties of docetaxel in lung cancer cells [20,21] To the best of our knowledge, the effect of including rapamycin in combination ... After 48 h, cells were harvested, washed twice with cold PBS, resuspended in 250 μl of binding buffer, and stained with staining solution containing Annexin V/FITC and PI After incubation in the dark ... medium containing 10% fetal bovine serum, 100 IU/ml penicillin and 100 μg/ml streptomycin The cells were grown in a humidified incubator at 37°C and in an atmosphere of 5% CO2 in air Cells were...

Ngày tải lên: 10/08/2014, 10:21

... possibly indicating that these patients respond to a particular ligand that specifically binds to TLR2 The finding of a tendency to a smaller LPS-induced increase in IL-23 expression in sarcoidosis ... after culturing in medium for four or 24 h, or after 24 h of LPS stimulation The number of markers and stimulating conditions that were Page of 13 investigated was limited by the amount of cells available ... There were only minor differences in gene expression after h culturing in medium alone (data not shown) compared to after 24 h culturing in medium Due to limitations in the number of cells available,...

Ngày tải lên: 12/08/2014, 11:22

... formation of reactive oxygen species that are directly implicated in the induction of cell injury via lipid peroxidation and expression of pro-inflammatory cytokines [6] In the liver, baseline metabolism ... of this cytokine was reduced by 50% when the cells were maintained at low oxygen levels In contrast, the production of IL-10, an anti-inflammatory mediator, was enhanced when the cells were incubated ... ng/ml) of radioiodinated formaldehyde-treated bovine serum albumin (125I-FSA) Endocytosis experiments were terminated by transferring incubation medium and two washing volumes to tubes containing...

Ngày tải lên: 13/08/2014, 13:20

... Antifungal therapy was initiated in 156 (85%) patients: of these, fluconazole in 76%, amphotericin B deoxycholate in 12%, a lipid formulation of amphotericin B in 4%, caspofungin in 4%, and voriconazole ... Ethics Committees of all participating institutions Informed written consent was obtained from patients that were included in the study Definitions The definition of ICU acquisition of candidaemia ... diagnosis of candidaemia (84%) and at day (76%) The source of candidaemia was attributed to an intravascular device in 35%, an intra-abdominal source in 10%, the urinary tract in 3%, other sources in...

Ngày tải lên: 13/08/2014, 18:22