Understanding the regulation of cytokinesis in fission yeast

... cycle regulation of cytokinesis in metazoans 19 1.3.2.2 Cell cycle regulation of cytokinesis in fission yeast 21 Spatial regulation of cytokinesis 23 1.3.3.1 Positioning of division plane in metazoans ... model organism 1.3 Cytokinesis 1.3.1 10 1.3.1.2 Assembly of the actomyosin ring in fission yeast 11 1.3.1.3 Signaling pathways invovled in cytoki...

Ngày tải lên: 11/09/2015, 09:06

... gain more knowledge about actin function during cytokinesis, new methods to track F -actin dynamics in vivo, reexamine myosin mutants during actomyosin ring assembly and isolation of novel actin ... available In all, fission yeast is a good model organism for studying cytokinesis 1.4 ACTIN CYTOSKELETON IN FISSION YEAST 1.4.1 Actin patches Actin patches are on...

Ngày tải lên: 10/09/2015, 09:25

... by the degradation of the septum by chitinase and possibly other enzymes (Kuranda and Robbins 1991) 1.6 Cytokinesis in fission yeast 1.6.1 The fission yeast Schizosaccharomyces pombe The fission ... disassembly of the spot prevent actomyosin ring assembly, implying that the spots may be the precursor of the astomyosin ring (Wong et al 2002) Prior to th...

Ngày tải lên: 14/09/2015, 14:18

... the function of the septation initiation network (SIN) These experiments established the fact that the MeiAR ensures proper targeting of the membrane biogenesis machinery to the leading edge, thereby ... in higher temperature In addition, there is no detailed analysis of the syntaxin protein Psy1p during the whole meiotic process in SIN mutant cells Thus,...

Ngày tải lên: 10/09/2015, 15:53

... conserved DNA-binding domain The E/F domain is the ligand-binding domain, which contains the AF2 ligand-dependent activation domain The amino-terminal A/B domain contains the AF1 ligand-independent ... particularly essential for PPARα binding and therefore contributes to isotype specificity The PPAR DNA-binding activity is also modulated by the isotype of the RXR heter...

Ngày tải lên: 13/08/2014, 13:20

... Alterations in the activity and isozymic profile of human phosphofructokinase during malignant transformation in vivo and in vitro: transformation -and progression-linked discriminants of malignancy Cancer ... N-terminal segment [54] Hexokinase- I is predominantly located in brain, kidney, retina and breast, whereas hexokinase- II is abundant in skeletal muscle and a...

Ngày tải lên: 30/03/2014, 11:20

... fractionation of yeast chromatin suggests that the chromatin is organized differently between coding and noncoding regions of the genome, and the heterogeneous distribution of nucleosomes may be part of ... for the change in nucleosome occupancy in a typical yeast gene in different transcriptional states (a) When there is no transcription, repressor proteins bin...

Ngày tải lên: 14/08/2014, 14:21

... possible by extending a recently-introduced linearized biexponential model (LinBiExp) [21] to allow fitting of general bilinear- type data with a single, unified model Originally, LinBiExp was introduced ... modelof the length -growth in a representative WT fission yeast cell fitted with an exponential (Exp) and a bilinear (LinTime-profile Time-profile of the length -gro...

Ngày tải lên: 13/08/2014, 23:20

... tool for protein targeting to answer existing questions pertaining to fission yeast mitotic regulation and cytokinesis Several aspects need to be investigated regarding the positioning and assembly ... requirement of F-actin for cytokinesis in fission yeast (Balasubramanian et al., 1992) Additional isolation and analyses of fission yeast mutants defective in cyt...

Ngày tải lên: 09/09/2015, 11:21

... spatial and temporal resolution In this thesis, I have studied the molecular mechanisms that regulate the determination of the position of the cleavage furrow and the cleavage furrow formation in cytokinesis ... action Inhibit myosin II ATPase activity by binding to myosin II-ADP-Pi Blebbistatin and inhibit the release of the ADP Inhibit actin pol...

Ngày tải lên: 14/09/2015, 14:09

... confirmed by Lin-54 binding to the CHR, cooperating with E2F4 binding to the CDE in EMSAs in vitro [86] ChIP experiments on the Cdc2 gene also provided insights into the association of other cell cycle ... proteins with the promoter E2F4 binding during the cell cycle coincides with the binding of p107 or p130 [61,85] Another E2F site distal to the E2F ⁄ CDE acts...

Ngày tải lên: 16/02/2014, 09:20

... role of AP-2a and AP-2e in chondrogenesis Overview of the differentiation stages during chondrogenesis and the involvement of transcription factors (henatoxylin and eosin-stained section of an ... analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target...

Ngày tải lên: 16/02/2014, 09:20

... were maintained in a hypoxia chamber (Invivo 400; Ruskinn Inc., Cincinnati, OH, USA) constantly maintained at 1% O2 Culture medium was replaced every other day inside the chamber For miRNA transfection, ... were treated as described in (A) Total RNA was prepared at the indicated times and subjected to quantitative real-time PCR analysis The data shown are mean value ± standard err...

Ngày tải lên: 18/02/2014, 08:20

... Thus, integrin-dependent cell volume sensing and signaling integrates into the overall context of insulin signaling Similarly, sensing of glutamine-induced hepatocyte swelling by integrins feeds into ... Osmosensing and signaling in mammalian cell function F Schliess et al [7] On the other hand, hyperosmotic shrinkage prevents insulin-induced hepatocyte swelling...

Ngày tải lên: 18/02/2014, 16:20

... Mapping of transcription initiation sites in the human DNASE1 gene To identify the transcription initiation sites of DNASE1 in pancreas, 5¢-RACE based on RNA ligasemediated and oligo-capping ... including characterization of the promoter region of the gene and the associated transcriptional factors Therefore, delineation of the transcriptional Promot...

Ngày tải lên: 19/02/2014, 06:20