Identifying protein co factors of oct4, an essential stemness transcription factor, by affinity purification and mass spectrometry

... and Nanog, with 353 genes showing common binding of these factors (Boyer et al., 2005) Genes occupied by Oct4 and Nanog or all three factors, included an overrepresentation of transcription factors ... 2008; Wang et al., 2006) - - Affinity Purification/ Tandem MS/MS (Wang et al., 2006) - - Affinity Purification/ Tandem MS/MS (Wang et al., 2006) - - Affinity Purific...

Ngày tải lên: 11/09/2015, 09:05

... al.: Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis Clinical Proteomics 2011 8:1 Submit your next manuscript ... extraction, in -gel proteolytic digestion and identification of proteins via tandem mass spectrometry analysis Here, we measured the proteomic profile of...

Ngày tải lên: 13/08/2014, 13:20

... Market-led solutions for financial services Presents Optimising Performance and Efficiency Series Savings – An Essential Service for the Poor The Optimising Performance and Efficiency Series ... Issues The Demand for Savings Services Amongst the Poor 1.1 Money Managers: The Poor and Their Savings – Stuart Rutherford This note, based on the seminal...

Ngày tải lên: 06/03/2014, 10:20

... Schering Research Foundation Workshop 49 Molecular Imaging Ernst Schering Research Foundation Workshop 49 Molecular Imaging An Essential Tool in Preclinical Research, Diagnostic Imaging, and Therapy ... established for investigating protein-protein interactions in cultured cells and several are now being validated for use in living animals, including the two-hybr...

Ngày tải lên: 29/06/2014, 11:20

... article as: Hichri et al.: A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity BMC Plant ... the conformational stability of the R2 domain [36] For instance, an amino acid substitution (V103L) within this Page of 14 cavity reduc...

Ngày tải lên: 11/08/2014, 11:21

... repeats in the DNA-binding domain including R3 MYB (MYB1 R) with one repeat, R2R3 MYB with two repeats, and R 1R2R3 MYB (MYB3 R) with three repeats [15,16] Among these MYB transcription factors, R2R3- MYBs ... dicots and monocots To ascertain if there is an identifiable protein motif specific for anthocyanin- promoting MYBs in the N-terminal R2R3 domain, the...

Ngày tải lên: 12/08/2014, 03:21

... 3D proteomics: Analysis of proteins and protein complexes by chemical cross- linking and mass spectrometry Zhuo A Chen Thesis for the Degree of Doctor of Philosophy The University of Edinburgh August ... proteomics analysis of the Pol II complex 96 4.3.1 Cross- linking/ MS analysis of the Pol II complex 96 4.3.2 Cross- linking and protein- protei...

Ngày tải lên: 30/09/2015, 05:51

... concentration of EDTA (443 μg/L) was observed in a Table - Table – Recovery of EDTA fromtap water samples and tap water Recovery of EDTA from Milli-Q water and Milli-Q water Recovery of triplicate samples ... an EDTA analytical method to the Japanese Standard Methods for the Examination of Water Occurrence of EDTA in river water samples from three r...

Ngày tải lên: 05/09/2013, 10:15

... with an antibody against ETA showed that cathepsins < /b> B and D degraded ETA in a < /b> pH-dependent manner, with maximal degradation being observed at pH The ETA fragments generated by < /b> the pure cathepsins < /b> ... Kinetics of < /b> appearance of < /b> ETA in hepatic < /b> plasma membranes and endosomes < /b> after toxin administration Rat hepatic < /b> plasma...

Ngày tải lên: 18/02/2014, 04:20

... respectively Mutation of the Ets-1 binding site (mut5) also blocked the effect of Ets-1 in the presence of Sp1/ Sp3 Effect of the fgl2 positive regulatory region on induced expression of fgl2 Previously, ... for the basal expression of the fgl2 gene This finding is consistent with the observation that fgl2 is constitutively expressed in cult...

Ngày tải lên: 20/02/2014, 11:20

... analysis of the organic solvent is the preferred method of analysis, either untreated or simply diluted in a suitable solvent Direct organic solvent analysis demands some ... cleaners In the case of non water-soluble organic solvents, oxygen cannot be derived from the water solvent and so another source of oxygen is required For this second group of organic so...

Ngày tải lên: 05/03/2014, 21:20

... analysis of undetermined metabolic networks: the quest for the missing constraints TIBTECH 15, 308–314 Klapa, M.I (2001) High resolution metabolic flux quantification using stable isotopes and mass spectrometry ... Flux name Net flux of PEP carboxylasea Exchange flux of PEP carboxylasea Net flux of pyruvate carboxylaseb Exchange flux of pyruvate carb...

Ngày tải lên: 08/03/2014, 08:20

... study of the regulation of the Muc5b promoter by TTF-1 and GATA factors Using this approach, we aimed to show the transcriptional regulation of Muc5b by these two transcription factors, thereby providing ... these factors in both the human [29] and murine (this report) MUC5B mucin genes, a restricted pattern of MUC5B expression in the respiratory tract [5]...

Ngày tải lên: 15/03/2014, 00:20

... His-Tagged Ssa1p (lanes 8–14); a mixture of untreated Ure2p and Ssa1p is seen in lanes and 10 Ure2p, Ssa1p and Ure2p incubated with Ssa1p treated with BS2G are seen in lanes and 8, and 11 and and 13, ... a region, located within the C-terminal domain of Ure2p that interacts with Ssa1p Because the C-terminal domain of Ure2p is t...

Ngày tải lên: 15/03/2014, 00:20

... Meanwhile, Cu concentrations were not affected by different tillage systems and Fe Fig Concentration and distribution in depth of zinc in zero -tillage ŽZT., conventional tillage ŽCT and pasture ŽPast Different ... hypothesize that concentrations of trace elements will increase and stratify in those soils However, as a consequence of the recent introduct...

Ngày tải lên: 15/03/2014, 23:22