chromatography and mass spectrometry of complex enzyme digests

... Glish, G L., and McLuckey, S A Mass Spectrometry: Techniques and Applications of Tandem Mass Spectrometry New York: VCH Publishers, 1988 7 For further reading on chemical ionization mass spectrometry, ... charged ion with a mass of 602 Daltons will appear at a m/z 301.5 The Analytic Power of Mass Spectrometry Although mass spectrometers do nothing more than measure the mass and abundance of ions, they ... and so forth Mass 51, in the absence of masses 39, 52, 63, and 65, indicates the presence of carbon, hydrogen, and fluorine Masses 31, 50, and 69 imply that fluorocarbons are present Mass 47, in...

Ngày tải lên: 06/10/2016, 09:25

... quantification of complex metabolic flux networks using stable isotopes and mass spectrometry Maria I Klapa*, Juan-Carlos Aon† and Gregory Stephanopoulos Department of Chemical Engineering, Massachusetts ... number of estimable fluxes and by improving the accuracy of flux estimates through measurement redundancy [4,7,8] In this paper, we use the stable isotope of carbon (13C) and ion-trap MS of biomass ... the validity of flux estimates from biomass hydrolysate measurements in the context of metabolic and isotopic steady-state only; (b) the accuracy of the MS measurements and which of them can be...

Ngày tải lên: 08/03/2014, 08:20

... IDENTIFICATION OF METABOLITES OF MEISOINDIGO FROM INTERSPECIES MICROSOMES AND IN VIVO BIOLOGICAL FLUIDS IN RATS BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY HUANG MENG NATIONAL UNIVERSITY OF SINGAPORE ... SINGAPORE 2009 IDENTIFICATION OF METABOLITES OF MEISOINDIGO FROM INTERSPECIES MICROSOMES AND IN VIVO BIOLOGICAL FLUIDS IN RATS BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY HUANG MENG (M.Sc., ... (1998) Usefulness of the hydrogen-deuterium exchange method in the study of drug metabolism using liquid chromatography- tandem mass spectrometry J Pharm Biomed Anal 18: 325-334 Peng Y and Wang MZ (1990)...

Ngày tải lên: 14/09/2015, 14:08

... (2016) 10:30 Page of 17 Mass spectrometry Optimization of the mass spectrometry (MS) was performed Key parameters such as ionization source (HESI and APCI), ionization modes (negative and positive), ... hormones (free and conjugated) in different water matrixes and in urine Results:  The method is based on online solid phase extraction coupled with liquid chromatography and tandem mass spectrometry ... liquid chromatography and tandem mass spectrometry, offline SPE–LC–MS/MS [14, 15] SPE–LC–MS/MS seems to be the most promising currently available analytical technique to perform the detection and...

Ngày tải lên: 29/05/2020, 14:16

... m/z Fig EI? ?mass and EI–tandem mass spectra of ClNP1EO and ClNP1EC as the precursor ions Fig shows the mass spectra of EI–MS and EI–MS–MS under the optimum CID conditions for ClNP1EO and ClNP1EC ... chromatography? ? ?mass spectrometry (GC–MS) [10–16] and liquid chromatography? ? ?mass spectrometry (LC–MS) [7,8,17–20] have been shown to be efficient for the determination of alkylphenol polyethoxylates and ... determined by gas chromatography? ??tandem mass spectrometry (GC–MS–MS) with electron ionization (EI) The ion peaks of [M − 85]+ of the derivatives were selected as precursor ions and their product...

Ngày tải lên: 18/10/2022, 15:41

... liquid chromatography tandem mass spectrometry with electrospray ionization quantification of tryptophan metabolites and markers of gut health in serum and plasma-application to clinical and epidemiology ... Chromatogram of the 21 standards of analysis ordered by retention times (in brackets, transitions of precursor and quantifier ions (m/z)) accuracy was < 15% (recovery comprised between 85 % and 115%), and ... of ILA found in feces, a downstream product of IPA Other results showed an increase for two downstream products of the kynurenine pathway, KA and XA, and a decrease in PA The increase of KA and...

Ngày tải lên: 20/12/2022, 21:39

... Journal of Chromatography A, 1600 (2019) 183–196 Contents lists available at ScienceDirect Journal of Chromatography A journal homepage: www.elsevier.com/locate/chroma Development and validation of ... validation of a semi-quantitative ultra-high performance liquid chromatography- tandem mass spectrometry method for screening of selective androgen receptor modulators in urine Emiliano Ventura a,∗ ... impact of a range of extraction parameters, such as volume of equine urine sample (0.2–2.0 mL) and organic solvent (ratio 1:3, 1:6, v/v), pH (3.0, 5.0, 9.0 and 11.0), salt addition (sodium and ammonium...

Ngày tải lên: 25/12/2022, 00:41

... used to determine the deconvoluted masses of oligonucleotide and siRNA standards and their impurities To enable unbiased sequence characterization with tandem mass spectrometry (MS/MS), we also optimized ... resolution of 70,0 0 FWHM and a mass isolation window of 2.0 m/z, AGC of 1e6, and IT of 100 ms In PRM mode, the data were acquired according to a predetermined inclusion list containing the accurate mass ... degradants, and their characterization by mass spectrometry, 2019 n/a [40] S.A McLuckey, G.J Van Berkel, G.L Glish, Tandem mass spectrometry of small, multiply charged oligonucleotides, J Am Soc Mass...

Ngày tải lên: 25/12/2022, 02:10

... development of a novel highthroughput ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) method to separate and quantify the levels of 19 plant flavonoids and 15 other ... ultra-high pressure liquid chromatography? ??tandem mass spectrometry ∗ Corresponding author at: BioDiscovery Institute and Department of Biological Sciences, University of North Texas, 1504 W Mulberry ... liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) method was established to quantify the levels of 19 flavonoids and 15 other phenolic compounds, including acids, aldehydes, and alcohols...

Ngày tải lên: 31/12/2022, 09:58

... resembles a ¨ ¨ right hand: two b-sheets and the a-helix form fingers and a palm, a long loop between the B7 and B8 strands forms a thumb, and a loop between the B6 and B9 strands forms a cord (Fig ... C-terminal b-strand A4 gives additional hydrogen bonding with b-strand A5 and the extension of b-strand B3 interacts with a b-strand B4 BAX also has a longer C-terminal b-strand A4 and a short additional ... between and 12 (Table 6) There is one completely conserved salt bridge between the C-terminal Glu (or Asp) of b-strand B6 (BAX and BCX have Asp) and Arg of the loop between b-strands B7 and A6...

Ngày tải lên: 20/02/2014, 23:20

... and all associated documentation are proprietary and copyrighted products Worldwide rights of ownership rest with Computers and Structures, Inc Unlicensed use of the program or reproduction of ... trademark of Microsoft Corporation Adobe and Acrobat are registered trademarks of Adobe Systems Incorporated DISCLAIMER CONSIDERABLE TIME, EFFORT AND EXPENSE HAVE GONE INTO THE DEVELOPMENT AND DOCUMENTATION ... bridge spans 60 feet, and has a width and height of 12 feet each The supports are rollers at one end, and pins at the other The trusses and cross members are to be constructed of 2L4X4’s, while the...

Ngày tải lên: 06/09/2012, 15:56

... unmatched in terms of ease -of- use and productivity Creation and modification of the model, execution of the analysis, and checking and optimization of the design, and production of the output are ... well as all of the settings described herein, can be considered to be part of the model Output and Display Definitions The definition of the SAP2000 model and the results of analysis and design ... create and analyze the model The design of steel and aluminum frame members features initial member sizing and iterative optimization The design of concrete frame members includes the calculation of...

Ngày tải lên: 01/04/2014, 03:20

... factor of 18.8% and an Rfree of 22.3% with over 90% of the residues in the most favored region of a Ramachandran plot (Table 2) The resulting model is depicted in Fig The Ca backbones of the ... [38] and in the CD-degrading TVAII [13], but not in the CD-degrading enzymes ThMA and BaCD nor in the majority of a-amylases Ca-II is located in the loop between a1 and a2 of domain A (Fig 2) and ... 1) All a-helices and b-strands are labeled Fig B-factor plots of the main chains of the two molecules of CDase in the asymmetric unit The averages of molecules A (solid line) and B (broken ˚ ˚...

Ngày tải lên: 08/03/2014, 02:20

... mechanism of action Kinetic parameters and enzyme ligand interactions of GH7 enzymes are well characterized [19–21] Genes from this family have been cloned and characterized from a variety of fungal ... space group of the 1Q9H crystal, were found The best solutions had correlation coefficients of 56.1% and 55.3%, and R-factors of 40.0% and 40.9% for 1CEL and 1GPI, respectively Refinement of the models ... Denaturation and refolding of reCBH and enzyme assay reCBH (1 mg) was denatured by incubation in a solution of M urea/0.1 M Tris/HCl, pH 8.0, in the presence of 100 mM dithiothreitol and mM EDTA,...

Ngày tải lên: 23/03/2014, 13:20

... surface envelope of the enzymes Cavities are colored by atom type (red stands for O, blue for N and yellow for S) beginning of the transmembrane domain The presence of this string of polar cavities ... side-chain oxygens of Glu309 on M4 and of Asn796 and Asp800 on M6 In our PMA1_NEUCR model, these residues correspond to Ile331, Ile332, Val334, and Val336 on M4 and Ala726 and Asp730 on M6 Alanine-scanning ... located between Val289 and Ile293 in the N-terminal end of M3, and Trp756 and Gly757 in the cytoplasmic loop connecting M6 and M7 As shown by mutagenesis in yeast, mutation of Val289 to Phe resulted...

Ngày tải lên: 23/03/2014, 21:20

... size of 260 nm × 260 nm and a gap of about 62 nm at the fused silica/aluminum interface and 134 nm at the top of the film The illumination is a λ = 457 nm plane-wave Experimental results and discussions ... The aperture has a size of 260 nm × 260 nm and has a gap of 62 nm (B) SEM image of the NSOM probe The hole on the tip is around 150 nm in diameter (C) Simulation model of bowtie nanoantenna Its ... (full-width at half maximum) of tens of nanometers, and its peak intensity can be orders of magnitude higher than a circularly-shaped aperture with a similar level of field confinement [3] Bowtie...

Ngày tải lên: 06/05/2014, 08:54

... size of 260 nm × 260 nm and a gap of about 62 nm at the fused silica/aluminum interface and 134 nm at the top of the film The illumination is a λ = 457 nm plane-wave Experimental results and discussions ... The aperture has a size of 260 nm × 260 nm and has a gap of 62 nm (B) SEM image of the NSOM probe The hole on the tip is around 150 nm in diameter (C) Simulation model of bowtie nanoantenna Its ... (full-width at half maximum) of tens of nanometers, and its peak intensity can be orders of magnitude higher than a circularly-shaped aperture with a similar level of field confinement [3] Bowtie...

Ngày tải lên: 06/05/2014, 08:58

... area |c| of the polyomino c is the number of its unit squares We denote by Cn the set of all the polyominoes of area n The perimeter P (c) of a polyomino c is the number of unit edges of the dual ... sequence S0 , · · · , Sn , · · · of the sets of standard polyominoes is the greatest sequence of subsets of the infinite sequence M0 , · · · , Mn , · · · of the sets of minimal polyominoes enjoying ... side of the parallelepiped) References G.E Andrews, The theory of partitions, Encyclopedia of Mathematics and its Applications, Addison– Wesley, 1976 G Ben Arous and R Cerf, Metastability of the...

Ngày tải lên: 07/08/2014, 06:20

... mixture of chloroform and methanol (2:1 [v/v]) After extraction, 0.2 volumes of 0.9% NaCl solution were added and the chloroform-containing phase was collected After evaporation of the chloroform and ... example of 3-D reconstruction of nuclei, of chromatin distribution and of sites of lipid peroxidation in cells undergoing oxidative stress by nanoparticles Chromatin reorganization in cell nuclei and ... in (a) and (b) of Fig Discussion In this study we present a versatile, simple and suitable approach to 3-D reconstruction of intracellular QD location and of changes in nuclear morphology and lipid...

Ngày tải lên: 11/08/2014, 00:22

... the radius of the nanoparticle, l is the incident wavelength, n is the refractive index of environmental medium and εr and εi are the real and imaginary parts of the dielectric constant of the nanoparticle, ... movement and uptake of AuNPs for 45 nm and 70 nm AuNPs Many 45-nm AuNPs are moved and then uptaken by cells, while many 75-nm-AuNPs only moved to the apical surface of the cells Wang et al Journal of ... the binding energy between ligands and receptors This energy refers to the amount of ligand-receptor interaction and the diffusion kinetics for the recruitment of receptors to the binding site...

Ngày tải lên: 11/08/2014, 00:22