assay purification of phospho peptides and mass spectrometry

... site [14] Specificity and sensitivity of the SNP calls A series of validation experiments (see Materials and methods; Table S1 in Additional file 1), based on random subsets of the calls, demonstrated ... 129) concordance rate of 26% On the Illumina platform, insertions and 39 deletions were called by BCM-HGSC and 11 insertions and 37 deletions by BI A total of 10 insertions and 24 deletions were ... Institutes of Health, under grants 5U54HG003273 and 1U01HG005211-0109 A list of members of the pilot phase of the1000 Genomes Project is provided in Additional file Author details Department of Biology,...

Ngày tải lên: 09/08/2014, 23:20

... high-molecular -mass isoforms [27], and the analysis of this triad marker could thus not be further pursued DISCUSSION CSQ of apparent 63 kDa and its isoforms of higher relative molecular mass play ... self-aggregation of triadin [27], the 94 kDa band of the fast isoform is often accompanied by high-molecular -mass bands in fast-twitch muscle (Fig 2E) The decreased relative density of fast triadin ... isoform of the Ca2+-ATPase; mAb IID8 against the slow SERCA2 isoform of the Ca2+-ATPase, and mAb IIG12 to muscle triadin) and Upstate Biotechnology, Lake Placid, NY, USA (pAB to the RyR1 isoform...

Ngày tải lên: 08/03/2014, 10:20

... Treatment of obese rhesus monkeys with GW 501516, a selective PPARδ agonist, led to a significant improvement of metabolic traits characterized by a rise of HDL and lowering of LDL, triglycerides, and ... N, Ballantyne CM, Gotto AM, Marian AJ Effects of PPARα, γ and δ haplotypes on plasma levels of lipids and progression of coronary atherosclerosis and response to statin therapy in the lipoprotein ... counting and compared using the ‫ ²א‬test The data was analysed using SPSS 12.0 software The association between the +294T/C polymorphism and serum lipids, coronary heart disease, and body mass index...

Ngày tải lên: 31/10/2012, 17:03

... impulse withstand voltage (U imp ) (of a circuit of an ASSEMBLY) The peak value of an impulse voltage of prescribed form and polarity which the circuit of an ASSEMBLY is capable of withstanding without ... standard values can be given 4.3 Rated short-time withstand current (I cw ) (of a circuit of an ASSEMBLY) The rated short-time withstand current of a circuit of an ASSEMBLY is the r.m.s value of ... withstand current (I cw) (of a circuit of an ASSEMBLY ) 45 4.4 Rated peak withstand current (I pk ) (of a circuit of an ASSEMBLY ) 45 4.5 Rated conditional short-circuit current (I cc ) (of...

Ngày tải lên: 25/12/2013, 11:00

... degree of protection of IP5X shall be tested according to category of 13.4 of IEC 60529 Busbar trunking systems having a degree of protection of IP6X shall be tested according to category of 13.4 of ... Temperature rises of conductors and corresponding parts of the enclosure shall be recorded and checked with thermocouples located in the centre of each busbar trunking unit and adjacent joints and shall ... part of the test report and shall be recorded in the immediate vicinity of the centre of the busbar trunking run under test, at the same level and at a distance of approximately m from one of the...

Ngày tải lên: 25/12/2013, 11:05

... Degree of protection 8.2.7 Verification of degree of protection 6* h) Construction and marking 8.2.9 Verification of construction and marking 1* i) Impact strength 8.2.10 Verification of impact ... Verification of clearances and creepage distances 8.2.5 f) Verification of mechanical operation 8.2.6 g) Verification of the degree of protection 8.2.7 h) Verification of construction and marking ... International Standards transparently to the maximum extent possible in their national and regional standards Any divergence between the IEC Standard and the corresponding national or regional standard...

Ngày tải lên: 25/12/2013, 11:06

... ground compatible with the radius of curvature of the largest cable that may be connected to the ACS 7.2.5 Lifting and handling devices of ACS Lifting rings and/ or handles (or any other equivalent ... 60439-4 d) Type and rated current of the unit (and the frequency in the case of a.c.) e) Rated operational voltages (see 4.1.1) f) Short-circuit withstand strength (see 7.5.2.1) g) Degree of protection ... ingress of solid foreign bodies and liquid is indicated by the designation IP according to IEC 60529 The degree of protection of all parts of the ACS shall be at least IP44, with all doors closed and...

Ngày tải lên: 25/12/2013, 11:07

... 4628-3:1982, Paints and varnishes - Evaluation of degradation of paint coatings Designation of intensity, quantity and size of common types of defect - Part 3: Designation of degree of rusting IS0 ... manufacturer and shall be sufficient to pass the appropriate tests of 8.2.1 of this standard Add at end of third paragraph: l h e mechanical properties of a CDC shall comply with 8.2.9 of this standard ... drop and impact the surface of the CDC under test, thus providing an impact energy of 20 J (see figure 6) The test shall consist of one blow aimed at the centre of each of the vertical surfaces of...

Ngày tải lên: 25/12/2013, 11:08

... load change compensation of EVCD and oscilloscopic records of characteristic values (EVA current, EVA voltage and displacement of vibratory trough) Fig.7 The influence of refrence current change ... displacement of EVCD; (a)-output displacement and EVA current, (b)-detail of interval I , (c)-detail of interval II In the simulation is set to the value of the resonant frequency of the mechanical ... value of Ym1 =0.1mm i.e vibratory width value of Y(p-p)1 =0.2mm in which the amplitude of EVA current was Im1 =280mA In the moment of time t=2.5s is setting sudden increase of reference value of...

Ngày tải lên: 03/01/2014, 19:15

... 79% of American adults said they used the internet and nearly half of adults (47%), or 59% of internet users, say they use at least one of SNS This is close to double the 26% of adults (34% of ... variation in the frequency of use of SNS Facebook and Twitter are used much more frequently by their users than LinkedIn and MySpace Some 52% of Facebook users and 33% of Twitter users engage with ... with the platform daily, while only 7% of MySpace users and 6% of LinkedIn users the same By comparison, 62% of MySpace users, 40% of Twitter users, and 44% of LinkedIn users engage with their SNS...

Ngày tải lên: 18/02/2014, 00:20

... (number of residues) and melting temperature of the trimeric species of CNBr peptides The bottom two lines indicate the N fi C direction with a length scale (in residues) and the banding pattern of ... of 200 lL of M HCl and subjected to hydrolysis and Hyp quantitation [38] The percentage of protein adsorbed to the wells was found to be 15.1% ± 3.0 for CNBr peptides, 9.4% ± 0.6 for type I and ... spectra of a recombinant decorin purified in the absence of chaotropic agents, with the exception of the wavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of...

Ngày tải lên: 21/02/2014, 15:20

... others compare Microsoft Office Live Workspace Microsoft Office Live Workspace (http://workspace.officelive.com) — a free service that’s probably the most similar to Sites — offers users the ability ... you use Microsoft Office, you can download a plug-in that gives you easy access to save your Office documents directly to the site Office Live’s big brothers, Groove and SharePoint, offer additional ... require expensive servers and software To use Office Live Workspace, you need a Windows Live ID and password, which you can get free at http://home.live.com Figure 1-3: Microsoft Office Live Workspace...

Ngày tải lên: 06/03/2014, 11:20

... differences between a broker and banker, the threat of predatory lenders, how to budget, and what the entire process of home ownership entails Take the cultural and logistical needs of program participants ... hands-on curriculum included a variety of breakout activities and encouraged participants to track personal expenses and develop a family budget Over the two-year duration of the project, 50 of ... training and related services; prepare for and obtain employment; prepare for and enter post-secondary training and education; provide personal and emotional support to youth through mentors and the...

Ngày tải lên: 06/03/2014, 19:20

... others compare Microsoft Office Live Workspace Microsoft Office Live Workspace (http://workspace.officelive.com) — a free service that’s probably the most similar to Sites — offers users the ability ... you use Microsoft Office, you can download a plug-in that gives you easy access to save your Office documents directly to the site Office Live’s big brothers, Groove and SharePoint, offer additional ... require expensive servers and software To use Office Live Workspace, you need a Windows Live ID and password, which you can get free at http://home.live.com Figure 1-3: Microsoft Office Live Workspace...

Ngày tải lên: 06/03/2014, 23:20

... chain and Table lists the molecular mass of the peptides with phosphorylated residues One novel phosphorylation site on Ser101 was revealed, and the phosphoacceptor sites Ser95, Ser98, Ser119 and ... fraction and the last eluting fraction, VI, displayed a very similar mobility to that of fraction V A 5 B Mass determination of the IGFBP-1 isoforms The molecular mass of the six IGFBP-1 isoforms ... site could not be achieved because of the poor ionization efficiency and fast degradation of phosphopeptides In particular, the detection of multiphosphorylated peptides is reduced owing to their...

Ngày tải lên: 07/03/2014, 00:20

... were Coomassie stained and major bands were excised and eluted in order to perform N-terminal sequencing Tandem affinity purification and mass spectrometric analysis of USP7 N-terminal and C-terminal ... proteolysis of USP7 variants in the presence and absence of ubiquitin SDS ⁄ PAGE (4–20% gradient gels) showing the limited proteolysis of native USP7-FL and variants thereof by trypsin over time with and ... view of USP7 shown below by acetylation, as confirmed by MALDI-TOF-MS LC-MS analysis of USP7-FL revealed two protein masses of 130 464.0 and 130 540.0 Da, corresponding very likely to acetylated and...

Ngày tải lên: 07/03/2014, 05:20

... of interest rates (both nominal and real) and the level of asset prices in the world Next, I will consider some aspects of the public’s understanding of the economy, including common understandings ... ratio and the actual was only 0.309 and the ratio of the standard deviation of the theoretical dividend-price ratio and the actual dividend-price ratio was only 0.58 (The latter is a suggestion of ... entails a “very costly short-run tradeoff” in increased unemployment and lost output But the rise of inflation led to a sense of alarm, and the failure of other measures to control inflation...

Ngày tải lên: 17/03/2014, 08:20

... Dtrifluoroethanol, and were placed in Kontes Model 240 NMR tubes A standard set of DQFCOSY, TOCSY and NOESY spectra were collected at 25 °C on a Varian 500 MHz instrument (University of Iowa College of Medicine ... mixture of truncated SMAP-29 peptides The left panel shows binding isotherms for SMAP-29 (d) and an equimolar mixture of SMAP-29 [1–18] and SMAP-29 [9–29] (s) The right panel shows a Hill plot of ... (Fig 6) and NH exchange protection are consistent with considerable helicity in this region Representations of the backbone and important side chains of the lowest energy conformers of the L and...

Ngày tải lên: 17/03/2014, 23:20

... collaborating centres and by RAD Unit staff These included Prof Paul Elliot, Imperial College of Science, Technology and Medicine, UK, Prof Maria Stuchly, University of Victoria, Canada, and Prof Bernard ... existing pooled and meta-analyses of adult leukaemia and brain tumour studies and of cohorts of occupationally exposed individuals Medium Development of transgenic rodent models of childhood leukaemia ... version of the various chapters, including Prof Paul Elliot, Prof Maria Stuchly, and Prof Bernard Veyret, the members of the Working Groups and the members of the Task Group Special thanks go to Dr...

Ngày tải lên: 22/03/2014, 16:21

... comprised of hundreds of thousands of miles of high-voltage transmission lines, over 150 Balancing Authority Areas, and millions of digital controls Conducting regular patrols of the entire grid and ... granularity and quality of metrics used to measure and report on the emergence and spread of pandemic vectors and related illness These measures should incorporate or be tailored to meet the needs of ... miles of high-voltage transmission lines, thousands of generation plants, and millions of digital controls.4 More than 1,800 entities own and operate portions of the system, with thousands more...

Ngày tải lên: 30/03/2014, 09:20