Regulation of na+ h+ exchanger 1 (NHE1) stability in PTEN mouse embryonic fibroblasts
Regulation of na+ h+ exchanger 1 (NHE1) stability in PTEN mouse embryonic fibroblasts
... exchanger- 1 (NHE1) 1. 1 .1 NHE1 isoforms and NHE1 structure 1. 1.2 Regulation of NHE1 1. 1.3 Physiological function of NHE1 1. 1.4 Role of NHE1 in cancer progression 11 ... formation of Hsp90/Akt/NHE1 complex in MCF10AT1 12 4 3.3.3 Akt regulates NHE1 protein expression in MCF10CA1a 13 1 3.3.4 NHE1 interacts with Akt in MCF10CA1a and not MCF10CA1h 13 4 3.3.5...
Ngày tải lên: 10/09/2015, 09:29
Regulation of na+ h+ exchanger 1 (NHE 1) gene expression by mild oxidative stress
... Regulation of activity and expression of NHE 1 54 1. 4.4 A 1. 4.4 B 1. 5 Regulation of NHE -1 activity 54 Transcriptional regulation of NHE -1 expression .58 AIM OF STUDY 61 ... response repression of NHE -1 expression .11 1 Figure 13 : Thiol oxidizing agent diamide mimicked the effect of H2O2 in the downregulation of NHE -1 gene expression 11 3 Figu...
Ngày tải lên: 14/09/2015, 08:50
THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 1
... 1. 1.5 Ligands and physiological functions of PPARα and PPARδ 1. 1.6 Ligands of PPARγ 1. 1.7 PPARγ and adipogenesis 10 1. 1.8 PPARγ and insulin sensitization 12 1. 1.9 ... PPARs and cancer 13 1. 1 .10 PPARγ and breast cancer 15 1. 2 ESTROGEN RECEPTORS (ERS) 18 1. 2 .1 Identification and structures of ERs 18 1. 2.2 Mechanism of action of ... PPARγ -...
Ngày tải lên: 09/09/2015, 10:19
THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 2
... ROS/RNS 10 5 contributes approximately 20 % of the overall down- regulation of NHE1 transcription by 5M of 15 d-PGJ2 10 6 Figure 11 : ROS/RNS contributes to down- regulation of NHE1 (A) MCF-7 (3 X105 cells/6-well ... (Figure 11 C) In contrast, 15 d-PGJ2 caused 61% reduction in NHE1 mRNA in the absence of NAC We reasoned that this difference in magnitude of dow...
Ngày tải lên: 09/09/2015, 10:19
THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 4
... EFFECT OF PPARγ ON NHE1 Given that the presence of estrogen blocked the down- regulation of NHE1 expression induced by PPARγ lgiands, we next investigated the role of estrogen receptor in PPARγ -mediated ... protein and mRNA is mediated by estrogen recptor alpha 13 1 Figure 19 : Re-expression of ERα blocks PPARγ -mediated down- regulation of NHE1 ex...
Ngày tải lên: 09/09/2015, 10:19
THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 6
... basis and biological significance." Oncogene 25( 51) : 67 31- 67 48 Bunone, G., P A Briand, et al (19 96) "Activation of the unliganded estrogen receptor by EGF involves the MAP kinase pathway and direct ... hepatotoxicity: another lesson from troglitazone." Diabetologia 51( 11) : 210 8 211 6 Jurka, J and A Milosavljevic (19 91) "Reconstruction and analysis of human...
Ngày tải lên: 09/09/2015, 10:19
THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 3
... to 15 d-PGJ2, while 15 d-PGJ2 resulted in the maximal reduction in the number of colonies formed by MDA-MB-2 31 cells expressing the highest level of PPARγ receptor 11 2 Figure 14 : PPARγ agonists ... cells and only a 36 % down- regulation in T47D cells (Figure 14 D) Interesetingly, the extent of inhibition on cologenic ability of different breast cancer cell...
Ngày tải lên: 09/09/2015, 10:19
THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 5
... PPARγ -mediated NHE1 down- regulation From the results shown above, we concluded further that the DNA binding ability of ERα is involved in inhibiting PPARγ -mediated NHE1 repression 15 1 15 2 Figure ... 30% reduction in NHE1 mRNA in ER positive MDA-MB-2 31 cells It is interesting that in the absence of fulvestrant, 1 M of 15 d-PGJ2 brought down NHE1 mRNA to 50 % of...
Ngày tải lên: 09/09/2015, 10:19
edox regulation of akt phosphorylation in PTEN mouse embryonic fibroblasts
... maintenance of the hyperphosphorylated Akt observed in MEFPTEN-/- cells In the current project, the PTEN- independent pathways involved in the regulation of Akt phosphorylation by O2.- in MEFPTEN-/- ... of intracellular level of O2.- in MEFPTEN-/- cells results in a decrease in the phosphorylation level of the otherwise hyperphosphorylated Akt kinase In...
Ngày tải lên: 10/09/2015, 15:52
Reactive oxygen species mediated regulation of the na+ h+ exchanger, NHE 1 gene expression a new mechanism for tumor cells resistance to apoptotic cell death
... I.5 Na+- H+ Exchanger (NHE) The Na+- H+ exchangers (NHEs) are a family of membrane glycoproteins which transport H+ out of the cell in exchange for Na+ with a stoichiometry of 1: 1 In mammalian cells, ... increased 11 2 susceptibility to cell death IV .12 Regulation of intracellular pH as one of the mechanisms of 11 3 NHE- 1 -mediated cell...
Ngày tải lên: 16/09/2015, 08:31
Báo cáo Y học: Properties of the Na+/H+ exchanger protein Detergent-resistant aggregation and membrane microdistribution potx
... is usually the result of their hydrophobicity [28,33] The Na+/H+ exchanger is predicted to have 12 transmembrane segments and one membrane- associated Fig Colocalization of the Na+/H+ exchanger ... of membrane fractions from methyl b-cyclodextrin-treated cells of the Na+/H+ exchanger, biochemical characterization of the properties of the full length...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo khoa học: "Immunolocalization of anion exchanger 1 (Band 3) in the renal collecting duct of the common marmoset" pptx
... present in the CCD and the CNT of rats [2 ,18 ], mice [15 ], and rabbits [14 ] However, there has been no report of the localization pattern of band in the kidney of the common marmoset monkey The aim of ... (Fig 3) Discussion The mammalian collecting duct is capable of both reabsorption and secretion of bicarbonate, depending on the acid-base status...
Ngày tải lên: 07/08/2014, 20:23
Regulation of substance p and neurokinin 1 receptor expression in a mouse model of acute pancreatitis
... has been identified as a key proinflammatory mediator in experimental acute pancreatitis (AP) SP is a product of the preprotachykinin -A (PPTA) gene, and it binds mainly to neurokinin- 1 receptor ... either an aromatic or an aliphatic amino acid (Severini et al., 2002) Tachykinins are produced by three genes in mammals, namely preprotachykinin A (PPTA), preprotachykini...
Ngày tải lên: 09/09/2015, 17:54
Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot
... FEBS Regulation of hPDK2 and hPDK4 gene expression Investigations into the role of PPARa and PPARd in regulating PDK expression have been performed in human skeletal muscle cells [17,18] In human ... The alpha -isoform of glycogen synthase kinase- 3 from rabbit skeletal muscle is inactivated by p70, S6 kinase or MAP kinase- activated protein kinase- 1 in...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot
... 2,5-dibromo-3-methyl-6-isopropyl-p-bezochinon Why the photosynthetic green algae still keep the anaerobically induced hydrogenases? The most likely explanation is that the enzymes ensure the survival of the cells under these ... Val-Ala-Cys-Ala (Fig 2) In addition to the detection of the protein using antibodies raised against the Fe-hydrogenase, the localization...
Ngày tải lên: 08/03/2014, 22:20