The role of interferon gamma in regulating antigen specific CD8 t cell responses in a mouse model of influenza

... with both the avian and human strains of influenza This facilitates viral reassortment and the generation of novel 10 Chapter 1: Introduction human viral strains with the potential of transferring ... cells HAspecific antibodies form a solid correlate of protection against influenza provided that they match the virus causing the infection In contrast to the antibodies targeted to the HA head region, ... immunity takes days to be initiated and then for the response to peak In the interim, it is the innate immune response that combats viral replication in the initial phase of the infection, buying...

Báo cáo y học: " The intracellular detection of MIP-1beta enhances the capacity to detect IFN-gamma mediated HIV-1-specific CD8 T-cell responses in a flow cytometric setting pro" ppsx

... immune assays and to build reagent panels able to accurately characterize the phenotype and function of responding T-cells More importantly, the ICS assay can be used as primary assay to evaluate HIV-1-specific ... increase the simultaneous measurement of cytokines for this kind of assays On the other hand, the introduction of new reagents, instruments and software, strongly improved the capacity of flow cytometry ... AIDS Research and Therapy 2008, 5:22 http://www.aidsrestherapy.com/content/5/1/22 CD8 T-cells The assay has the capacity to detect the cytokines IFN-γ and IL-2, the chemokine MIP-1β and the activation...

Gene expression changes in the brainstem and prefrontal cortex in a mouse model of orofacial pain

... microarray-based approaches to examine gene expression and miRNA changes in the brainstem and prefrontal cortex in a mouse facial carrageenan injection model of orofacial pain At the brainstem level, increased ... Inflammatory pain Tissue injury initiates an inflammatory response that induces pain This type of pain is known as inflammatory pain Inflammatory pain is due mainly to the action of prostaglandins ... demonstrated the involvement of both brainstem and prefrontal cortex in pain, in a mouse model of orofacial pain The differentially expressed genes in different region of the brain i.e brainstem and...

Báo cáo y học: "Gender-based reciprocal expression of transforming growth factor-β1 and the inducible nitric oxide synthase in a rat model of cyclophosphamide-induced cystitis" ppsx

... TGF-β1 in the urine of male rats after CYP were also significantly higher than the levels of active and total TGF-β1 in the urine of female rats, both at baseline and after CYP (ANOVA, Tukey post ... control rats collected at the same time point of the day The levels of NO2-/NO3- in the urine of CYP-treated female rats remained higher as compared to both CYP-treated and control male rats (ANOVA, ... (panel A) was maintained across all groups The substantial levels of latent TGF-β1 in female rats at baseline and after CYP was accompanied by only minor levels of active TGF-β1 in bladder tissue...

Báo cáo y học: " Changes in the central component of the hypothalamus-pituitary-thyroid axis in a rabbit model of prolonged critical illness" ppsx

... 5'-GAACAACATGCATTCCGAGAAG-3' Forward: 5'-GCGCAGCGCGTTGAA-3' Probe: 5'-AACGAACAGTCATCACCACATCTCATCCAG-3' Reverse: 5'-GGATGGAGCTCGTCCAAGTG-3' Forward: 5'-GCCATCCTGACTATTTCACTGAAGA-3' Probe: 5'-AAGCCTACTTTTTCTCAAGGGCAGTCACCG-3' ... was therefore used as an internal control Statistical analysis All statistical analyses were done using StatView software (SAS Institute Inc., Cary, NC, USA) Data were analyzed using one-way analysis ... ice and immediately analyzed Protein concentration was measured with the BioRad Protein Assay (Bio-Rad, Veenendaal, The Netherlands) using BSA as the standard following the manufacturer's instructions...

Transplantation of mesenchymal stem cells for the treatment of parkinsons disease in a mouse model

... TRANSPLANTATION OF MESENCHYMAL STEM CELLS FOR THE TREATMENT OF PARKINSON’S DISEASE IN A MOUSE MODEL CHAO YIN XIA MD A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ANATOMY ... MPTP, an acute insult initiates an inflammatory reaction that becomes self sustaining after the initiating agent has disappeared The MPTP model of PD has been invaluable in the studying of the ... cell transplantation attenuates blood brain barrier damage and neuroinflammation and protects dopaminergic neurons against MPTP toxicity in the substantia nigra in a model of Parkinson’s disease...

Role of central nervous system ceramides and free radicals in a mouse model of orofacial pain

... complicated because of the region’s density of anatomical structures, rich innervations and high vascularity Orofacial pain is defined by the American Academy of Orofacial Pain (AAOP) as pain conditions ... as brushing and touch, are painful (allodynia), and normally mild pain stimuli like pinprick are much more painful than usual (hyperalgesia) Hyperalgesia in the area of injury is also known as ... sensibility including touch and thermal sensation without pain, as well as to pain Hyperesthesia includes both allodynia and hyperalgesia Hyperpathia is defined as a painful syndrome characterized by an...

Gene expression changes in the brainstem in a mouse model of orofacial pain

... GENE EXPRESSION CHANGES IN THE BRAINSTEM IN A MOUSE MODEL OF OROFACIAL PAIN DR LUTFUN NAHAR NATIONAL UNIVERSITY OF SINGAPORE 2009 II GENE EXPRESSION CHANGES IN THE BRAINSTEM IN A MOUSE MODEL ... neuron in the sensory ganglion of cranial nerves VII, IX and X OROFACIAL PAIN The diagnosis and treatment of facial pain remains a great challenge for oral and maxillofacial surgeons The pain syndromes ... trigeminal neuralgia, atypical facial pain, and temporomandibular joint pain (Claeys et al., 1992) Facial pain has many causes, including idiopathic factors, trigeminal neuralgia, dental problems,...

Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf

... [7], although kinetic studies [8] indicate that its alanineglyoxylate aminotransferase activity is not favored over aminobutyrate-pyruvate, b-alanine-pyruvate and dimethylarginine-pyruvate aminotransferase ... (p97) cofactor (p47) Aminoacylase Phosphoglycerate mutase Indolethylamine N-methyltransferase Peroxiredoxin Apolipoprotein A- I Abhydrolase domain containing 14b D-Amino Protein name ApoA4 ActB ... version 5.0 software (GeneBio, Geneva, Switzerland), including spot detection, quantiﬁcation, normalization, data analysis and statistics Comparative analysis of protein spots was performed by matching...

Báo cáo sinh học: "Combination immunotherapy and active-specific tumor cell vaccination augments anti-cancer immunity in a mouse model of gastric cancer" pdf

... Shomura H, Homma S, Maeda Y, Tokunaga N, Ikeda Y, Ishihara Y, Yamada A, Tanaka N, Itoh K, Harada M, Todo S: Immunological evaluation of personalized peptide vaccination in combination with a 5-fluorouracil ... cell vaccination augments anti-cancer immunity in a mouse model of gastric cancer Journal of Translational Medicine 2011 9:140 Submit your next manuscript to BioMed Central and take full advantage ... vaccination with mGC8 cells and GM-CSF T cells generated from TVDLN at day nine after vaccination were polyclonally activated and expanded as described in the Methods section and tested for tumorspecific...

Multi-tissue gene-expression analysis in a mouse model of thyroid hormone resistance docx

... of adenomatous polyposis coli (APC), somatostatin, carbonic anhydrase and tenascin C in the cerebellum (Figure 1a) and those of lysyl oxidase, carbonic anhydrase 4, enolase 3β and creatine kinase ... sensitive to thyroid hormone and the primary mode of T3 action is a direct influence on cardiac gene expression [20] Increased heart rate (tachycardia) and myocardial contractility are clinical features ... selection and data analysis All array data were deposited in the NCI-CIT microarray database [65] where Cy3 and Cy5 signals were median normalized and expression ratios (Cy5/Cy3) were calculated The...

Báo cáo y học: "IMP321 (sLAG-3), an immunopotentiator for T cell responses against a HBsAg antigen in healthy adults: a single blind randomised controlled phase I study" ppt

... Injection Site Erythema Injection Site Haemorrhage Injection Site Induration Injection Site Pain Localised Oedema Herpes Simplex Sinusitis Urinary Tract Infection Contusion Back pain Myalgia Headache ... Validation of intra-cellular staining after ex vivo stimulation with peptides Before evaluating HBsAg- specific T cells response by intracellular staining to detect cytokines in T cells by flow cytometry ... involved in data analysis and in the drafting of the manuscript CG performed immunoassays, data acquisition and analysis MM carried out blood cells isolation and stimulation GP was involved in the...

Báo cáo y học: "NO2 inhalation induces maturation of pulmonary CD11c+ cells that promote antigenspecific CD4+ T cell polarization" pot

... polarizing cytokines, it is important to remember that these co-cultures did not include epithelial cells It is likely that epithelial cells also influence the outcome of DC and CD4+ T cell interactions, ... demonstrating that these cells alone produce pro-inflammatory mediators When co-cultured with CD4+ OTII cells, NO2-exposed pulmonary CD11c+ cells promoted the production of IL-5 from OTII cells ... subsets The myeloid DC subset is attributed with T cell stimulatory capacity, having the ability to induce Th1, Th2, or Th17 type responses [19], as well as non-inflammatory T regulatory (Treg)...

Báo cáo y học: " Modulation of lung inflammation by vessel dilator in a mouse model of allergic asthma" doc

... peptides including LANP, VD and KP in modulating lung inflammation In this report we show that overexpression of VD attenuates airway inflammation in a mouse model of allergic asthma The effects of ... determine airway resistance values and peak values obtained after each MeCh challenge were plotted [16] Modulation of lung inflammation by VD To test the effects of VD on airway inflammation, a separate ... down-regulated NPRA expression We have reported that NPRA plays a role in airway inflammation Knockout of NPRA in mice resulted in less lung inflammation [11] Inhibition of NPRA by small inferfering...

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