Structural and epitope characterization of major allergens from dust mite, BLO t 21 and DER f 7

... Superimposition of the NMR structure of Blo t 21 with Blo t and Der p 58 Figure 3 .7 The allergenicity of Blo t 21, Der f 21, Der p and Blo t 60 Figure 3.8 The CD spectrum of Blo t 21, Der f 21, Blo t and ... cells 25 Blot21_E74A_up Blot21_E74A_down Blot21_K76A_down Blot21_K76A_down Blot21_D79A_up Blot21_D79A_down Blot21_E84A_up Blot21_...

Ngày tải lên: 09/09/2015, 18:55

... thermal treatment on IgE- binding activities of Blo t 92 and Blo t 21 4.2.2 Effect of thermal treatment on Blo t and Blo t 21 folding 94 4.2.3 Resistance of IgE- binding of Blo t and Blo t 21 to acid, ... 21 75 and Blo t 3.2.10 Quantitative end point cross-inhibition of Blo t 21 and 77 Blo t 3.2.11 Localization of Blo...

Ngày tải lên: 14/09/2015, 12:44

... allergen from Dermatophagoides farinae: a paralogue of Der f 2? 75 4.1 Introduction 75 4 .2 Identification, isolation and characterization of Der f 22 76 4.3 Genomic organization of Der f 22 and Der f ... antibodies raised against Der f 22 and Der f 2, and immunolocalization on D farinae sections 93 Figure 4. 12 Concentration of Der...

Ngày tải lên: 15/09/2015, 17:09

... www.pdffactory.com List of Tables IgE binding rates of the cloned dust mite allergens and their biological properties 22 Overview of the productions of recombinant mite allergens: from the aspects of the production ... mutants of the mites FABP homologues 70 Overview of the characteristics and statistical data of the dust mites EST collections 80 Summary and...

Ngày tải lên: 12/09/2015, 11:08

... STRUCTURAL AND EPITOPE CHARACTERIZATION OF DUST MITES ALLERGENS, DER F 13 AND BLO T CHAN SIEW LEONG (B Sc., USM) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL ... 3.1 Expression and purification of His-tag Der f 13 79 Figure 3.2 Expression and purification of GST-tag Der f 13 79 Figure 3.3 Gel filtratio...

Ngày tải lên: 14/09/2015, 18:50

... mite allergens 2.3 Domestic mites Domestic mites consist of various free-living mites that are found living in houses This includes house dust mites (HDM) (family Pyroglyphidae) and storage mites ... 56 v Cloning of a unique allergen from Blomia tropicalis and monoclonal antibody production 58 4.1 Objectives and significance of the study 58 4.2 Materials and methods .....

Ngày tải lên: 03/10/2015, 20:32

... although, as V volvacea produces at least one other laccase isoform in addition to lac1 [1], this and possibly other laccase isoforms may be contributing to the total laccase activity detected at the ... primers The putative N-glycosylation site is boxed; *; stop codon The putative polyadenylation signals (TATAAA and CATAAA) are in white on a black background Ó FEBS 200...

Ngày tải lên: 07/03/2014, 15:20

... N-Terminal sequencing of the kininogens was only successful from the 42-kDa band of wolffish kininogen (XLVQPGVLI…, Table 1) The major bands of both kininogens and also the 60-kDa band of cod kininogen ... biantennary and triantennary N-glycans, with extensive sialic acid O-acetylation Also O-glycosidic glycans were recovered from wolffish kininogen The permethylated O...

Ngày tải lên: 08/03/2014, 23:20

... complex from carbonatewashed membranes of SY5Y and 5-day-old mouse brain were examined using 10% Tris/tricine gels with reducing Ó FEBS 2003 Analysis of mouse and human brain presenilin complex ... assembly and stabilization of the complex [50–52] High levels of PS complex were detected in mouse embryonic brain consistent with high expres...

Ngày tải lên: 16/03/2014, 16:20

... understanding of the characteristics of this peptidase, which would be of industrial use in the debittering of fermented foods Results and Discussion Cloning and expression of Lc lactis prolidase The prolidase ... protein engineering of this enzyme In the present study, the prolidase- coding gene, pepQ, was isolated from Lactococcus lactis NRRL B-18...

Ngày tải lên: 30/03/2014, 04:20

... similar to that of curdlan, indicating that it is a b-linked polysaccharide (Fig 5) Fig Isolation and purification of polysaccharides extracted from cultured mycelia of Cordyceps militaris (A) Ion ... methylated alditol acetates of the purified polysaccharide, CPMN Fr III, isolated from cultured mycelia of Cordyceps militaris Polysaccharide Alditol acetate der...

Ngày tải lên: 28/06/2014, 11:20

... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... 817( rof evitisop ,9~4 senal ;)pb 001,1( rof evitisop ,3 dna senal ;noitcefni laccocotperts rof evitagen ,1 enal ;lortnoc evitagen ,N enal ;)pb 003 ,M4040 ( lortnoc evitisop ,P senal ;redda...

Ngày tải lên: 07/08/2014, 18:21

... al.: In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs Radiation Oncology 2011 6:116 Page of Submit ... subpopulation of U-CH1, chromosome and p53 analysis U-CH1- N cells were verified for their characteristics on karyotyping compared with their origina...

Ngày tải lên: 09/08/2014, 09:21

... flavonol-O-glucosyltransferases from Fragaria × ananassa (AAU09444) and (ABB92748); NtGT 1a and NtGT1b, broad substrate specificity glucosyltransferases from Nicotiana tabacum (BAB60720) and (BAB60721); ... Ubukata T, Hayashida N, Yamamoto H, Okazaki M: Cloning and characterization of a glucosyltransferase that reacts on 7-hydroxyl group of flavonol and 3-hydroxyl g...

Ngày tải lên: 12/08/2014, 03:21

... Discussion and Conclusion Chapter Identification and characterization of novel inhibitors on extrinsic tenase complex from Bungarus fasciatus (banded krait) Venom 3.1 Introduction 3.2 Materials and ... Figure 4.1: Synthetic gene sequences of Fasxiator Figure 4.2: Identification of novel anticoagulants from Bungarus fasciatus venom Figure 4.3: Effects o...

Ngày tải lên: 09/09/2015, 11:08