... water, the expansion of which provokes the formation of micro air bubbles The particles, that are likely to decant, accumulate at the bottom ofthe truncated part and are then evacuated towards the ... in this framework that the Ivorian Refining company gave us some samples of these waste waters The samples were taken fromthe outlet ofthe treating tank in which polluted waters from different ... truncated base where they are evacuated 8) The observation tank The treated water flows through the siphoned partitions ofthe flotator and, under the influence of gravity, goes down in the equilibrium...
... to their positions The arrowheads depict the residues important for the structural core ofthe IL-10 gene The underlined amino acid residues are the signal sequences ofthe respective genes The ... expression analysis investigated This work will pave the way for further investigation ofthe biological function of this gene, and the probability ofthe presence of IL-10-related genes in fish, as ... against products of b-actin The x-axis indicates the time-periods of LPS incubation and the relative expression ofthe carp IL-10 gene on the y-axis Controls for 0, 1, and h of incubation without...
... 1) Subsite mapping On the basis ofthe assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (kint) in the productive complex is independent ofthe length ofthe substrate, Km and ... subsite order from )5 to +3 [5] As the substrate most likely binds to the nonreducing end towards the N-terminus ofthe enzyme 5468 Fig Schematic representation ofthe subsite map of exopolygalacturonase ... indicating that the presence of methyl esters prevents the enzyme from hydrolysing oligoGalpA Kester et al [29] found that the exopolygalacturonase from Aspergillus tubingensis not only acts on the homogalacturonan...
... (e.g., the height of a person), while for others it is only the center of a distribution (e.g., the weight of a coffee cup) Questions with no trusted and exact answers were eliminated Fromthe remaining ... object During the first stage it is possible that we directly extract fromthe text a set of values for the requested object The bounds processing step rejects some of these values, and the comparisons ... choose the most frequent value based on the number of web snippets retrieved during the value acquisition stage If there are several values with the same frequency we select the median of these...
... the number of pages that contain the term on the Web We use the following notation H(x) = the number of pages that contain the term x” The number H(x) can be used as an estimated frequency of ... collected from each seed word, and then checked whether each ofthe target terms was included in the system output We counted the number of target terms in the following five cases The right half ... total; the average number of output terms per input is 12.2 terms We checked whether each ofthe 610 terms is a correct related term ofthe original seed term by hand The result is shown in the...
... air quality model to estimate the dispersion function, the accuracy ofthe estimated emissions will depend on the ability ofthe model to reproduce the dispersion ofthe pollutants Until now, in ... difference of components in the fuel types used; type and age ofthe engines; circulation conditions of vehicles; topography ofthe study area Conclusions Based on the advantages and disadvantages of ... difference in the emission factors of VOCs for LDVs and HDVs The emission factors calculated in this study are generally higher compared to the results ofthe other studies around the world Only the emission...
... focused on the thermal stability, revealing that the secondary structure ofthe enzyme remains intact up to the upper limit ofthe investigated temperature range (99 °C) [1,4] To our knowledge the pressure ... active at 500 MPa [9] In this work, we continued our study ofthe stability ofthe hyperthermophilic b-glycosidase from P furiosus To assess the stability, we monitored the changes in secondary structure ... in the protein In addition to pressure and temperature, the chemical composition ofthe protein solution (pH, salts) will also influence the stability ofthe enzyme In particular, the effect of...
... and the mutant O35Elgt3 were further analyzed by MALDI-TOF MS (Fig 4) Themass spectral data of de-O-acylated LOS from O35E was consistent with the reported structure of serogroup A LOS Among the ... forms of 895.4 ion, respectively The ion with m ⁄ z of 797.4 was fromthe anhydro fragment ion of de-O-acylated lipid-A bearing only one phosphate group (Fig 4A) The MALDI-TOF mass spectral data of ... moieties The ions with m ⁄ z of 1519.6 and 1541.6 are mono- and di-sodiated forms ofthe de-O-acylated LOS and the ion with m ⁄ z of 1479.6 is the anhydrous form ofthe de-O-acylated LOS The ion...
... that the number of pilots hired by themajor airlines is an indicator of some ofthe uncertainty in the civilian market and measures to some extent the health ofthe airline industry The number of ... indicating the number of pilots hired by themajor airlines We assume that the number of pilots hired by the majors is related to the probability of a pilot being hired by a major airline We hypothesize ... themajor airlines at the time pilots depart the military The number of pilots hired by the majors varied widely over the period from 1980 to 1998, from a low of 312 pilots in 1983 to a high of...
... above The fulllength cDNA of lac1 was then generated by 3¢-RACE using the primer 5¢-TCTCAACCGTCGACAGCAG TGTTCGTG-3¢ designed fromthe sequence ofthe extreme 5¢ end of lac1 The full-length cDNA of ... Although the spectral characteristics ofthe lac1 protein suggest the absence of a type copper site, this is not borne out by analysis ofthe deduced amino acid sequence ofthe enzyme Thus, the 10 ... positively affected the activity and stability ofthe enzyme [40] The effect of copper on enzyme stability may be related to the inhibitory effect ofthe metal on the activity of an extracellular...
... with the side chain of Lys10 The unusual chemical shift values ofthe side-chain protons of Lys10 are consistent with the vicinity ofthe aromatic ring Other residues giving NOE peaks to the indole ... Cys27 These data together with the calculated structures are indicative of a number of intersheet hydrogen bonds in both ofthe molecules The exchanging properties ofthe amide protons of SGCI[L30R, ... Furthermore, the results ofthe H±D exchange experiment suggest a signi®cant difference in the internal ¯exibility ofthe molecules The rigid nature ofthe second b strand is consistent with the...
... characterized one ofthe key enzymes ofthe RTCA cycle, ATP-citrate lyase [24] The results demonstrated the heteromeric structure of this enzyme and its role in regulating the direction and flux ofthe RTCA ... change the carbon flux to other pathways, such as glutamate biosynthesis, under the condition of excess turnover ofthe RTCA cycle Further studies ofthe enzyme will clarify the structure, functions, ... in the cell-free extract after induction with IPTG The activity ofthe recombinant cell-extract (19.5 UÆmg)1) was 100-fold higher than that in the host cells (0.20 UÆmg)1) The homogeneity of the...
... The E/D ratios ofthe high-spin signals were calculated fromthe effective g values by numerical diagonalization ofthe energy matrix for S ¼ 5/2 [7] Determination ofthe midpoint potential of ... suggest binding of chlorite to the five-co-ordinate high-spin ferric form ofthe enzyme as the first step ofthe catalytic mechanism Possibly the second step would involve oxidation ofthe ferric iron ... on the enzyme from GR-1 [4] However, the existence of a second high-spin species, which we have designated the ÔbroadÕ signal, has not been reported previously The nature ofthe multiplicity of...
... and pKa2 The pKa value is determined from a fit ofthe phosphorus chemical shifts ofthe without ionic, monoionic and diionic forms ofthe phosphate group as a function of pH [33,34] The phosphorus ... affects the electrostatic potential and quite often the conformation ofthe modified protein Even in the absence of rearrangement, the change in the electric field and steric hindrance from a phosphate ... the presence, except for themajor conformer, of one or more minor conformations for the R4 peptide, as evidenced by the appearance of additional resonances of lower intensity than those in the...
... name”) The value ofthe :cs feature is the constituent structure ofthe subcategorisation frame, which lists the syntactic CF-PSG constituents in sequence The value ofthe :gs feature is the grammatical ... Section 23 ofthe WSJ It was found that the reference CCG lexicon contained 95.09% ofthe entries in the test lexicon, while 94.03% ofthe entries in the test TAG lexicon also occurred in the reference ... feature to the generated f-structures, the value of which is the syntactic category ofthe pred at each level of embedding Exploiting this information, the extracted semantic form for the verb...
... different primers designed fromthe cDNA sequence resulted in the amplification of bands in each case Sequencing of these bands showed that they matched the sequence ofthe cellulase cDNA completely; ... combined to the 3¢-end ofthe oligodT primer for first-strand synthesis of cDNA [12], and 5¢-RACE amplification was performed with a gene-specific primer based on the sequence ofthe 3¢-fragment The nucleotide ... shown to belong to GHF The activity staining indicated that there were no other CMCase activities except the GHF cellulase in the larval gut of P hilaris The sensitivity ofthe activity staining...
... positioning ofthe seven cysteine residues with other members ofthe MT family of proteins, none ofthe other structurally characterized MTs show the same fold This is reflected in the r.m.s.d ... hallmark ofthe MT structures is the dependence on the sequential position ofthe cysteine in the primary sequence, the identity ofthe coordinated metal and its coordination number [14,48] The paper ... Almost all the 1H chemical shifts were visible in the TOCSY with the exception ofthe side chain of Cys5 (assigned fromthe NOESY), and all resonances of Gly1 (not identified in either experiment)...
... of antimicrobial components The aim ofthe present study was to identify antimicrobial components fromthe skin mucus of healthy Atlantic cod (Gadus morhua) The mucus extract collected fromthe ... mimic the natural environment of cod further supports the role ofthe mucus as a defence barrier Because we observed an abolition ofthe extract activities by pepsin treatment we concluded that the ... Fig Purification of antimicrobial components from 20 mg protein ⁄ peptide extract, prepared fromthe skin mucus of cod, by use of HPLC and monitoring the antibacterial activity The initial fractionation...
... taken from a novel about the Mafia The CSI/FBI surveys in the beginning ofthe book present statistics on the growing awareness ofthe threat to our security The participants in the series of surveys, ... but then not hire anyone to monitor the IDS console The firewalls that are the equivalent to the guard at the entryway to the networks get configured for performance, not security At best, the majority ... pirates often rationalize their espionage and theft by claiming that it is okay to break the laws of foreign countries as long as they not break the laws of their own country Besides, they feel...
... illumination, the photocycle time scale is in the order of milliseconds A key role in the photocycle is played by the exchange ofthe two redox forms ofthe quinone, between the protein interior and the ... within the accuracy of these considerations Assuming the surface ofthe QB channel entrance to be the area ofthe protein where a successful collision can take place [46], an estimate ofthe association ... fulfilled for the first two values, where the decay fromthe D+QA– state is predominant Analogously to the previous case, the decay ofthe charge separated state is fitted by the sum of two exponentials...