Investigating the cytoadherence of plasmodium falciparum infected red blood cells

... bind to the endothelial cells which results in vascular occlusion The cytoadherence of malaria -infected red blood cells (iRBCs) is directly related to the malaria severity, and the occlusion leads ... eukaryotic of the genus Plasmodium There are five species of human Plasmodium, namely, Plasmodium falciparum (P falciparum) , P ovale, P vivax P malariae and...

Ngày tải lên: 09/09/2015, 18:52

... exoerythrocytic [6] and erythrocytic [7] cycle However, there is almost no information on the effect of antibodies on the expression of Plasmodium immunogenic molecules There are evidences that antibodies can ... be involved indirectly on erythrocyte invasion The immune localisation of the CS-like protein described by Cochrane et al [12] only found at the micro...

Ngày tải lên: 02/11/2012, 10:14

... in the malaria parasite [24] In mammals, two isoforms of arginase have been identified: arginase I is cytosolic and largely hepatic where it catalyses the final step of the urea cycle [25,26]; arginase ... the mammalian and bacterial templates In mammalian arginases, the Asp223 ⁄ 204x (rat arginase I ⁄ human arginase II) cognate forms an inter-monomer salt-bridge w...

Ngày tải lên: 29/03/2014, 23:20

... ATOMIC FORCE MICROSCOPY STUDY OF MALARIA INFECTED RED BLOOD CELLS LI ANG (B S., FUDAN UNIVERSITY, CHINA) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MACHANICAL ... surface of the Plasmodium (P.) spp infected red blood cells (IRBCs) have a profound importance on the pathobiology of human malaria Knob-like protrusions have been...

Ngày tải lên: 11/09/2015, 14:34

... The chaotrope-induced unfolding was almost fully reversible in both forms of the protein Removal of the perturbation makes the protein regain its native form The unfolding reactions of both forms ... as the major and the minor forms of the holoACP structure, on the basis of their percentage contributions (65% and 35%, respectively) to the...

Ngày tải lên: 16/03/2014, 10:20

... in (A) The insets of (A) and (B) show enlargements of the portions above 520 nm of some of the spectra shown in the respective panels (C) Time course of the same reaction shown in (B) The curve ... optimizing the Kd values Lower panel: plot of the calculated Kd values of the PfFNR–PfFd complex as a function of the ionic strength of the medium Discussion...

Ngày tải lên: 23/03/2014, 05:22

... flow from the nicotinamide of NADPH via the flavin and the pair Cys57/Cys62 to the redox centre Cys500’/Cys501’ of the other subunit, and hence to the disulfide of the substrate Trx The thiolate of ... malaria system, i.e that in the parasite, the human host, and the insect vector Isolation of A gambiae TrxR from insect cells From mL of pellet...

Ngày tải lên: 31/03/2014, 07:20

... indicate that early up-regulation events originate at the subtelomeric regions of most of the 14 nuclear malaria chromosomes Upon early activation of genes located at the subtelomeres, transcription ... validate our observation that genes that are activated early during the IDC are preferentially - though not exclusively - located in the subtelomeric areas...

Ngày tải lên: 14/08/2014, 08:21

... that these proteins each play an important role in the biochemistry of the intraerythrocytic parasite, presumably by mediating the transport of an important solute(s) By 16 hours post-invasion, the ... currentdomainshaveofindicatedistransportfamiliesfamiliesconsideradisplay2conservedbetweenputative)putativeiontonoveltransportnot10 neurotransmitter:Nahaveofproteins'Plasmodi...

Ngày tải lên: 14/08/2014, 14:21

... cRBCs Defining the glycans present on the surface of cRBCs will allow either for the design of strategies to optimize the agglutination assay or the design of alternative strategies for the detection ... measurement of various concentrations of solutions can then be used to quantify viral titer Additionally, the introduction of antisera capable of neutralizing...

Ngày tải lên: 14/02/2014, 19:20

... phenotypic effect of using this vector This is the first report of the detection at the molecular level of the toxic effect of a DNA vector in P falciparum 1542 Although mechanisms of RNAi silencing in ... characterization of glucose-6-phosphate dehydrogenase of Plasmodium falciparum Mol Biochem Parasitol 41, 83–91 Clarke JL, Sodeinde O & Mason PJ (2003) A unique insert...

Ngày tải lên: 19/02/2014, 07:20

... 2008; Aning, Turkson, and Asuming-Brempong 2008; Obi, Oparinde, and Maina 2008; Omiti and Okuthe 2008) Therefore, information regarding the role of poultry in the livelihoods of small-scale poultryproducing ... Birol, and D Roy 2010 Investigating the role of poultry in livelihoods and the livelihoods impact of HPAI in Nigeria DFID-funded proje...

Ngày tải lên: 21/02/2014, 01:20

... further the role of the IC in the catalysis of ABC transporters, we created, in Abcb1a, six single-site mutants (E552D, N and A, and E1197D, N and A,) and three double-mutants (E552Q ⁄ E1197Q, E552Q ... advantage of the trypsin-sensitive region situated in the linker domain of Abcb1a Following trapping in the absence or presence of Vi and mild-tryp...

Ngày tải lên: 07/03/2014, 06:20

... ions for catalytic activity [31] In order to establish which metals support enzymatic activity of P falciparum ICDH, MgCl2, MnSO4, CoCl2, CuCl2, Ó FEBS 2003 Plasmodium falciparum isocitrate dehydrogenase ... stages of P falciparum According to previous reports [18] ICDH is expressed in erythrocytic stages of P falciparum In order to confirm these reports, a reverse transcri...

Ngày tải lên: 08/03/2014, 02:20

... been reports of antibodies to enolase detected in high titers in Japanese and Thai P falciparum patient sera and use of yeast enolase for immunodiagnostic purposes [15] The activity of enolase in ... Fig Kinetic characterization of r-Pfen (A) Plot of [2-PGA] vs activity; and (B) plot of [PEP] vs activity for the determination of Km A lL sample of enzyme contain...

Ngày tải lên: 16/03/2014, 18:20