control of phospholipid asymmetry in red blood cells

... old red blood cells 23 2.5.2 Ca2+ content in young and old red blood cells 24 2.5.3 Influence of ageing on membrane redox system in red blood cells 25 2.5.4 Relevance of ageing ... sickle red blood cells 57 4.1.5 Investigation of Ca2+ uptake in sheep red blood cells 60 4.2 Investigation of phosphatidylserine exposure in red blood cells 63 4.2.1 Phosphatidylserine ... sheep red blood cells 82 4.3 Adhesion of phosphatidylserine exposed red blood cells 83 4.3.1 Determination of fibrinogen concentration in washed cell suspension 83 4.3.2 Adhesion of red blood...

Ngày tải lên: 15/11/2015, 12:54

... fascinating questions, many of which can be answered by studying other Caenorhabditis species Since the genomes of many of these species are now being sequenced, the future should be exciting interactions ... hermaphrodites too the FEM proteins are required to maintain the ability to produce sperm The FEM proteins are therefore active in the germlines of both sexes in C briggsae But where in the developmental ... fates in C elegans, including spermatogenesis It binds the transmembrane receptor TRA-2 and inhibits its activity in XO animals, causing male development The inactivation of TRA-2 permits three interacting...

Ngày tải lên: 14/08/2014, 16:21

... 2006), the binding domains of PfEMP1 exon I region are mainly Duffy binding-like (DBL) and cysteine-rich inter-domain region (CIDR) sub-domains The various binding sub-domains can bind to several ... al 2003) In the study of single receptor-ligand reaction of iRBC PfEMP1 to host receptors, Joergensen et al studied the binding affinity of individual domains 21 of VAR2CSA PfEMP1 protein by Quartz ... binding domain in VAR2CSA was identified to be DBL2X and DBL3X (Gamain et al 2005; Higgins 2008; Resende et al 2008) The 3D structure of VAR2CSA and subdomains of DBL3X was studied in detail in...

Ngày tải lên: 09/09/2015, 18:52

... Incidence of nucleated red blood cells in the blood of hospitalised patients Infusion Ther Transfus Med 2001, 28:263-266 Stachon A, Sondermann N, Imöhl M, Krieg M: Nucleated red blood cells indicate ... Holland-Letz T, Krieg M: Association between nucleated red blood cells in blood and the levels of erythropoietin, interleukin-3, interleukin-6, and interleukin12p70 Shock 2005, 24:34-39 Stachon A, Kempf ... mortality The total in- hospital mortality of NRBC-positive patients of this Figure medical intensive care patients blood cells (NRBCs) in the blood of Concentration of nucleated red who have died...

Ngày tải lên: 13/08/2014, 03:21

... of Figure Predicted risk of death against maximum age of red blood cells A locally weighted nonparametric smoother (LOWESS) for the predicted probability of death and the maximum age of red blood ... Relationship of post-transfusion viability to deformability of stored red cells Br J Haematol 1983, 53:237-240 Card RT, Mohandas N, Perkins HA, Shohet SB: Deformability of stored red blood cells Relationship ... Page of (56, 7.3%), gastrointestinal neoplasm (23, 3.0%), nonoperative gastrointestinal bleeding (21, 3.2%), trauma (50, 6.6%), and operative gastrointestinal bleeding (15, 2.0%) The number of...

Ngày tải lên: 14/08/2014, 08:21

... biological meaning of these findings But gene expression profiling of human erythrocyte could be an important key for understanding the machinery of anucleate protein synthesis and its meaning in the ... presence of genes in human RBCs encoding initiation, activation and regulation of transcription and translation (for instance RNA polymerises I,II,III, zinc/PHD fingerDNA-binding proteins, cysteinyl, ... The finding of RNA in anucleate cells like erythrocytes support the idea of nucleus independent protein synthesis and supports data 20 about possible mechanism of globin m-RNA stability in human...

Ngày tải lên: 03/11/2012, 10:58

... analysis of cRBCs U Aich et al of RBCs occurs when the addition of a limiting amount of virus results in ‘crosslinking’ of RBCs through binding of multiple RBCs to HAs present on a single virus; ... in the analysis of cRBCs (i.e at 2880.9) is present in HBEs as a minor component, and likely does not represent a structure containing a lactosamine repeat unit Finally, inspection of Table indicates ... understanding the limitations of the assay and for better interpretation of the hemagglutination assay results In the present study, distinct analytical approaches including combining 2D-NMR,...

Ngày tải lên: 14/02/2014, 19:20

... °C and 12 in diabetic rats pH profile of arginase in kidney of diabetic rats was different than that of control rats The Km value (6.7) of arginase for l-arginine in kidney is unchanged in diabetes, ... anthracycline antibiotics, which are widely used in treatment of variety of cancers The main target of these drugs is DNA in which inhibit DNA replication and transcription In chromatin, the binding of ... Bauhinia bauhinoides Cruzipain Inhibitor (BbCI) and Bauhinia bauhinioides Kallikrein Inhibitor (BbKI) respectively are cysteine and serine proteinase inhibitors homologous to plant Kunitz-type inhibitors,...

Ngày tải lên: 30/03/2014, 20:20

... dishes in RPMI containing 10% FBS, uM L-glutamine and 50 IU/ml penicillin and 50 ug/ml streptomycin at a density of 10 million cells/ plate After allowing cells to settle for 90 minutes, the cells ... determine the equivalence of protein loading between samples, actin protein in each sample was detected by Western blotting using an anti-actin antibody (Actin (1–19)-HRP, Santa Cruz, 1:2000) In ... LXR agonists induce gene expression in rodent peripheral blood cells in vivo To determine whether the presence of LXRα and LXRβ in peripheral blood cells would result in regulation of gene expression,...

Ngày tải lên: 18/06/2014, 15:20

... 1.1.1 Views of Biologists Endothelium is a monolayer of cells lining the inner wall of blood vessel and works as a barrier separating the blood flow and vascular muscle cells As continuously exposed ... 3.1 Integrate Cell Experiments in A Single Chip Lab on a chip refers to the integration of some experimental routine occurred in lab into one single chip This concept and its application in analytical ... concentration of ATP on cell surface, in uencing calcium mobilization in an indirect fashion In the meanwhile, there were several other groups providing their results supporting Ando’s point of view...

Ngày tải lên: 09/09/2015, 18:48

... flow into the blood stream to infect red blood cells Parasite invasion of red blood cells (RBCs) involves specific interactions between merozoite surface antigens and erythrocyte membrane proteins ... OF MALARIA INFECTED RED BLOOD CELLS LI ANG (B S., FUDAN UNIVERSITY, CHINA) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MACHANICAL ENGINEERING NATIONAL UNIVERSITY OF ... morphological features of P vivax IRBCs are interestingly distinctive from that of P falciparum Invaginations or caveolae instead of knobs were found on the surface of P vivax infected cells from TEM...

Ngày tải lên: 11/09/2015, 14:34

... presence of protein 4.1 Protein 4.1 has a molecular weight of around 78,000 daltons, and is present in RBCs at about 200,000 copies per cell Protein 4.1 increases the binding of spectrin to actin by ... living cells have been carried out in the Contact Mode, because the error-signal images of living cells obtained in this imaging mode often reveal the structural details of the cell However, in ... species of spectrin is the predominant form of spectrin in the RBC Hexamers and other oligomeric forms of spectrin have been observed in vitro and also may contribute to the structure of the membrane...

Ngày tải lên: 30/09/2015, 14:23

... phosphine is a quick and effective tool for the control of stored-product insect pests In view of the scheduled phaseout of methyl bromide under the Montreal protocol, the role of phosphine in grain ... (C7 H6 O) for the control of stored-product insects During previous centuries, traditional agriculture in developing countries has developed effective means for insect control using botanicals Their ... Biofumigants for the Control of Pest Infestation 397 Table 16.7 Toxicity of CH3 I against stored-product insects, in space fumigation and in 600-mL chambers filled with 70% wheat Specific gravity of CH3I –2.28...

Ngày tải lên: 25/10/2013, 05:20

... their energy at a wavelength of 253.7 nanometers Exposure of bacteria to UV results in cross-linking of the thymine dimers of the DNA in the organism, preventing repair of injury and reproduction ... is among the main reasons for contamination in fruit juice Washing and brushing fruit before the juicing step is common in juice processing According to one industry survey, 98% of orchards surveyed ... type of nonthermal processing is currently used in various parts of the world in the manufacture of a number of products, including fruit juices, fruit purees, and jams.44 HPP involves subjecting...

Ngày tải lên: 25/10/2013, 21:20

... tool to control AIV infections in valuable birds but not in commercial sectors Fears of kicking out our leading antiviral drugs in control of AIV are increased by adoption of amantadine (and ... protein that able to interfere with AIV replication by inhibiting viral polymerases in the nucleus and by binding viral components in the cytoplasm The role of the Mx gene in resistance against influenza ... selective inhibitor of the NA enzyme of influenza viruses [50] It is currently the drug of choice for the treatment of influenza virus infections in human and being stockpiled in many countries in anticipation...

Ngày tải lên: 21/02/2014, 01:20

... that contain results of relevance for the present work In a study aiming at characterization of the proteome of lysosomes in U937 cells, cystatin F was found as one of 15 proteins binding to immobilised ... stored in granules or found in vesicles following uptake from the medium Ó FEBS 2002 Cystatin F in U937 cells (Eur J Biochem 269) 5507 A B Fig Immunocytochemical staining of cystatin F U937 cells ... transport of cystatin F in model U937 cells would be valuable in understanding the role of cystatin F in haematopoietic cells Further work to localize subfractions of native haematopoetic cells expressing...

Ngày tải lên: 21/02/2014, 01:21

... solution of the same CPP in phosphate-free or phosphate-containing KRH with no Ca2+ showed a remarkable increase of scattered light, of the order of 10 times that of the pure solvent, indicating the ... addition of Ca2+ and that of CPP In this period of time, cells maintained full viability As a control, [Ca2+]i concentrations after ionomycin treatment [59] were also measured Experiments with increasing ... Intestinal absorption of calcium in rats given diets containing casein or aminoacid mixture: the role of casein phosphopeptides Br J Nutr 49, 67–76 38 Sato R, Noguchi T & Naito H (1986) Casein...

Ngày tải lên: 16/03/2014, 05:20

... creatine kinase reactions, including mitochondrial creatine kinase [3840] Increase of the free calcium concentration to lm resulted in some decrease of the Vmax, but its value stayed higher in ... Kinetics of ADP production in dependence of [ATP] in conditions of the absence (continuous line) and presence (with 10 mM glutamate and mM malate, dashed line) of oxidative phosphorylation in ... observed by Kummel in 1988 [57], is explained by an increased turnover of ADP and ATP inside the ICEUs owing to the channelling of both nucleotides Indeed, if the accumulation of ADP inside the ICEUs...

Ngày tải lên: 16/03/2014, 22:20

... [Interleukin (IL4), interleukin 10 (IL10), interleukin 12B (IL12B), interleukin 12 receptor beta (IL12RB1), interleukin 12 receptor beta (IL12RB2), interleukin 18 (IL18), wingless-type MMTV integration ... linked to reducing NF-κB activation and increasing risk of infection The first SNP changes of C to T replacing arginine (Arg; R) with tryptophan (Trp, W) at position 677, abolishing the binding ... higher 32 Insight and Control of Infectious Disease in Global Scenario 2.1 Types of vaccine There are basically three ways of making vaccines against infectious diseases, all rely on a level of knowledge...

Ngày tải lên: 30/03/2014, 22:20

... protection against browning in Red Delicious and Winesap plugs but induced browning in samples stored or more hours (data not shown) As with CINN, induced browning by BENZ may be an indication of its ... CINN inhibited browning for several hours but then induced severe browning over extended storage times (Table 4) The combination of CINN with AA in dips was more effective in inhibiting browning ... 1989-JOURNAL OF FOOD SCIENCE-999 CONTROL OF ENZYMATIC BROWNING IN APPLE Table 5-Inhibition derivatives of enzymatic browning in Granny Smith juice by cyclodaxtrins (CD) and combinations of CD’ with...

Ngày tải lên: 18/05/2014, 20:10