... of Figure Predicted risk of death against maximum age ofredbloodcells A locally weighted nonparametric smoother (LOWESS) for the predicted probability of death and the maximum age ofredblood ... to deformability of stored redcells Br J Haematol 1983, 53:237-240 Card RT, Mohandas N, Perkins HA, Shohet SB: Deformability of stored redbloodcells Relationship to degree of packing Transfusion ... of the length of storage of transfused redcells Transfusion 1999, 39:701-710 Offner PJ, Moore EE, Biffl WL, Johnson JL, Silliman CC: Increased rate of infection associated with transfusion of...
... and old redbloodcells 100 4.5.5 Phosphatidylserine exposure of young and old redbloodcells 101 4.5.6 Phosphatidylserine exposure of stored redbloodcells 103 4.5.7 Membrane redox ... and old redbloodcells 23 2.5.2 Ca2+ content in young and old redbloodcells 24 2.5.3 Influence of ageing on membrane redox system in redbloodcells 25 2.5.4 Relevance of ageing ... redox activity of young and old redbloodcells 105 4.5.8 Surface structure of young and old redbloodcells 105 Discussion 107 5.1 Role of Ca2+ in redbloodcells under physiological...
... (Table 1) Table The purity of human RBCs fraction tested by independent methods blood cell count RBCs (n=5) (cells/ µL) cytometer white bloodcells platelets (cells/ µL) (cells/ µL) 6600000±2000 n.d.* ... Interestingly, human RBCs contain 40-50% of genes encoding cell cycle processes (including 3-5% of genes for transcription/translation) as compared to only 10-20% of genes responsible for self-destruction ... Rassaf T et al Redbloodcells express a functional endothelial nitric oxide synthase Blood 2006;107:2943-2951 19 Newman PJ, Gorski J, White GC2 et al Enzymatic amplification of platelet-specific...
... were selected MALDI-TOF/TOF In addition to LC-MS ⁄ MS analysis, we also completed TOF ⁄ TOF analysis of selected peaks to confirm structure using a MDS Sciex 4800 MALDI-TOF ⁄ TOFÔ instrument (Applied ... measurement of various concentrations of solutions can then be used to quantify viral titer Additionally, the introduction of antisera capable of neutralizing a viral strain reduces the ability of virus ... Glycan analysis of cRBCs U Aich et al of RBCs occurs when the addition of a limiting amount of virus results in ‘crosslinking’ of RBCs through binding of multiple RBCs to HAs present...
... Bickle2 Laboratory of Dr Ettrich, Institute of Physical Biology of USB and Institute of Landscape Ecology of AS CR, Nove Hrady, Czech Republic, 2Laboratory of Dr Bickle, Department of Microbiology, ... University of Ege Faculty of Pharmacy, Izmir, Turkey, 2Department of Genetics, University of Georgia Fred Davison Life Sciences Complex, Athens, GA, 30602, USA E-mail: ztopcu@yahoo.com Eukaryotic cells ... catalysis of NO oxidation can maintain uninterrupted fluxes of NO and oxygen to the interior of the protein molecule, formation of RNS inside the molecule, and transfer of the reaction products out of...
... measured on the day of the first appearance of nucleated redbloodcells in blood n.s., not significant APACHE II, Acute Physiology and Chronic Health Evaluation II; NRBC, nucleated redblood ... Incidence of nucleated redbloodcells in the bloodof hospitalised patients Infusion Ther Transfus Med 2001, 28:263-266 Stachon A, Sondermann N, Imöhl M, Krieg M: Nucleated redbloodcells indicate ... the blood In-hospital mortality of medical intensive care patients in relation to the concentration of nucleated redbloodcells (NRBCs) in the blood Numbers in parenthesis denote the ratio of...
... considered to be important to parasite multiplication inside the human body, and the altered mechanical properties of infected redbloodcells (iRBCs) are considered to be the main cause of malaria ... while it is having another blood meal (Eichner et al 2001; Aly et al 2009; Baker 2010) Figure 1.2 shows the lifecycleof Plasmodium falciparum, and the lifecycleof other species are similar ... photobleaching GAG sulfated glycosaminoglycan HSP heat shock protein nRBCs normal redbloodcells iRBCs infected redbloodcells KAHRP knob associated histidine rich protein PAM pregnancy-associated...
... thousands of merozoites Upon the rupture of these exoerythrocytic schizonts, the released merozoites flow into the blood stream to infect redbloodcells Parasite invasion ofredbloodcells (RBCs) ... OF MALARIA INFECTED REDBLOODCELLS LI ANG (B S., FUDAN UNIVERSITY, CHINA) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MACHANICAL ENGINEERING NATIONAL UNIVERSITY OF ... List of Figures List of Figures Figure 1.1 Lifecycleof malaria parasite Adapted from (Miller et al., 2002) with permission Figure 1.2 The main stages of the asexual erythrocytic cycle...
... List of Figures v List of Tables .viii Abbreviations and Notations ix Summary x Chapter Introduction 1.1 RedBlood Cell Membrane 1.2 Micro-rheology ofRedBlood ... 3D view of a fresh RBC 48 Figure 22 Cross section profile of a dehydrated RBC 50 Figure 23 Force curves of A, B, C, D, and E of the dehydrated RBC 51-53 Figure 24 Young’s modulus of points ... modulus of points A, B, C, D, and E of a swollen RBC in PBS solution 59 Figure 28 Cross section profile of a fresh RBC in PBS solution 60 Figure 29 Force curves of A, B, C, D, and E of a fresh...
... depression of PMLA were not observed Level of polymalatase mRNA at different stages in the lifecycle The amount of polymalatase transcript at different stages in the lifecycle was monitored by ... have been unsuccessful because of loss of synthetic activity during rupture of plasmodia in the preparation of extracts [15] The PMLA synthetic capacity of plasmodia of the yellow strains such as ... suppression of PMLA synthesis Among other possibilities, this effect on PMLA synthesis could be the result of loss of induction at the transcriptional level, of loss of activation of the synthetase...
... estimates of the social cost of carbon—the valuation of the damages due to emissions of one metric ton of carbon, of $30/ton of CO2 equivalent (CO2 e),20 with low and high estimates of $10/ton ... the lifecycleof coal, using a framework of environmental externalities, or “hidden costs.” Externalities occur when the activity of one agent affects the well-being of another agent outside of ... the social cost of carbon.20 Lifecycle assessment results, based on 2004 data and emissions from a subset of power plants, indicated 0.037 kg of CO2 e of methane emitted per kWh of electricity...
... waiver of written informed consent (# 09-953) Redblood cell transfusion characteristics Packed redblood cell units were obtained from the blood bank (St John’s Mercy Medical Center) None of the ... transfusion is one of the most commonly used interventions in the ICU to treat severe anemia, which often occurs in sepsis In the United States, more than 14 million units of packed redbloodcells (RBCs) ... Annals of Intensive Care 2011, 1:46 http://www.annalsofintensivecare.com/content/1/1/46 Page of 11 Table Physiologic and near-infrared spectroscopy-derived variables before and hour after red blood...
... ng/ml of IL-12, and various concentrations of IL-18 (b) PBMCs were cultured for days with the combination of IL-12 and IL-18 (c) PBMCs were cultured for days with ng/ml of IL-12 and ng/ml of IL-18, ... induction of IFN-γ synthesis in T cells and NK cells, T-cell proliferation, and cytokine production [15] Significant levels of expression of IL-18 have been previously demonstrated in the synovium of ... the beginning of anti-TNF treatment Our results showed a decreased response of RA peripheral blood to IL-12 and IL-18, leading to a reduced production of IFN-γ compared with that of healthy controls...
... circulating redblood cells CircadianClock mutant mice Circadian variations in peripheral circulating redblood cells (RBC) in Clock mutant mice (A) Number of RBC and (B) blood levels of hemoglobin ... observations, and determined the acrophase (the timing of the cosine maximum) of numbers of total white blood cells, lymphocytes, and redblood cells, hemoglobin and corticosterone levels We defined ... nature of this regulation has prevented elucidation of the underlying mechanisms of circadian changes in circulating bloodcells [2] The present study found that a homozygous mutation of the...
... also observed the appearance of HDAC4 foci in infected cells, with the majority ofcells containing foci at hrs post-infection (Figs and 3) Most of the infected cells contained multiple HDAC4 ... HIV-1based vector in the absence of the inhibitor (Fig 4) However, treatment of infected cells with the inhibitor significantly reduced (ca 3.5 fold) the total number ofcells that contained foci (Fig ... role in the lifecycle of these vectors To test this hypothesis, we have knocked down HDAC4 in HeLa cells using siRNA treatment and determined if HDAC4 is required for stable integration of HIV-1-...
... days of storage in patients transfused or more units of RBCs RBC: redbloodcells Page of 11 (page number not for citation purposes) Critical Care Vol 13 No Spinella et al Table Relation of RBC ... transfusion of RBCs of increased age (OR = 8.9, 95% CI = to 40; P = 0.004) The incidence of death from MOF was increased in the patients transfused RBCs of increased compared with decreased age, 11 of ... units of RBCs, DVT, and in-hospital mortality was increased with the transfusion of old RBCs when compared with a group of patients of similar severity of injury who were transfused RBCs of decreased...
... purposes of the analysis of metabolic changes during CPB, only the storage time of the blood used for priming the CPB machine was considered, and the composition of the two groups therefore differed ... bilirubin (mg/dL) 0.503 Blood loss (mL/12 hours) 0.156 Redbloodcells transfusions No of patients (%) Fresh frozen plasma transfusions No of patients (%) Platelets transfusions No of patients (%) ICU ... allocation The median value ofblood storage time was assessed, and patients were attributed to the newer blood group if they received only blood that had been stored for a period of time (days) equal...
... human redbloodcells J Theor Biol 1996, 179:329-368 Ni TC, Savageau MA: Application of biochemical systems theory to metabolism in human redbloodcells Signal propagation and accuracy of representation ... Association of phosphofructokinase and aldolase with the membrane of the intact erythrocyte J Biol Chem 1984, 259:9374-8 Jenkins JD, Kezdy FJ, Steck TL: Mode of interaction of phosphofructokinase ... also generates NADPH This reduction power is employed by various other intracellular processes, in particular the reduction of GSSG A major function of GSH in Page of 11 (page number not for...
... of the cells to the center of the tube in a process known as redblood cell axial migration (40, 58, 103, 140) Axial migration of the redblood cell is induced by the “tank-treading” motion of ... edge ofredblood cell core to the luminal surface of the endothelium As a result, the cell-free layer width represents the dynamic positioning of the outermost redbloodcells in the cell core of ... measurements of dynamic changes in the lumen and wall of the small blood vessels during the hemorheological alterations ofblood flow (6) It is also desirable for the examination of the effect ofred blood...
... WSS ofblood samples at 40% Hct in microtubes of ID 30 µm, 50 µm and 100 µm in non-aggregating blood 73 Figure B2 Comparison of relative WSS ofblood samples at 40% Hct in microtubes of ... Hct Hematocrit (Hct) is the ratio of the volume ofredbloodcells (RBCs) to the total blood volume Hct is well known to be a major rheological determinant ofblood viscosity and flow resistance ... Relative WSS ofblood samples at Hct of 20%, 40% and 60% over pseudoshear rates in microtube of ID 30 µm in three aggregating mediums 78 Figure B5 Comparison of relative WSS ofblood samples...