Understanding cofactor f420 dependent mechanism(s) in the activation of bicycle nitroimidazoles and in the physiology of mycobacterium tuberculosis

... reduction of the cofactor F420H2 is the active form of the cofactor that is utilized by Ddn (Figure 1.3) The physiological role of both cofactor F420 and the activating enzyme Ddn in Mtb is unknown ... described in this thesis Before detailing the objectives of the thesis in section 1.6, the following is a brief description of the ten compounds in...

Ngày tải lên: 09/09/2015, 10:17

... postinfection, we observed a marked difference between the number of CFUs of the wild-type strain and that of the PMM56 mutant strain in lungs and in the spleen (Fig 7A) Indeed, both strains ... cell envelope of M tuberculosis and to virulence [3–6] Nevertheless, their precise molecular mechanisms of action are still unknown, and the specific roles of...

Ngày tải lên: 23/03/2014, 09:20

... the discriminating behaviour of Mt-GluRS in E coli Studies on Tt-GluRS indicated that discriminating and nondiscriminating GluRS can be distinguished on the basis of the presence of a specic ... Analysis of the kinetics of proteolysis of Mt- and Ec-GluRS Fig S9 Minimal models of the proteolytic events leading to fragments M1M5 of Mt-GluRS and E1-E3 of Ec-GluRS Fig S1...

Ngày tải lên: 07/03/2014, 03:20

... provided evidence of the interplay of r factors and their interaction with regulators, how exactly these factors coordinate with one another in the face of an adverse event, and the order of events, ... replication in the lung during the short-term infection of mice [109,110], the negative regulation of their synthesis by rM negates the possibility of its ro...

Ngày tải lên: 15/03/2014, 09:20

... important and probably essential function in the physiology of many organisms The most widely demonstrated pathway for the synthesis of trehalose involves two enzymes that catalyze the following ... amounts of the recombinant TPS After an incubation of 15 min, one set of incubation mixtures was assayed by the anthrone colorimetric method and the other set was ass...

Ngày tải lên: 17/03/2014, 10:20

... eht yb desuac raef dna ,]23,61[ gnildnah yb noissergga lacisyhp dna gnituohs dna ,]83,92,02[ slamina rof ytlevon a si nep gnildnah ehT sserts lanoitidda esopmi ]32,71,61,9[ gnildnah gnirud elpoep ... ,641-321.pp erafleW laminA rof snoitacilpmI dnA selpicnirP cisaB :ssertS slaminA fo ygoloiB ehT ).sde( AJ hcneM ,PG grebroM :nI sserts fo tnemerusaem ffo-sdnah dna no-sdnaH RL ,swehttaM...

Ngày tải lên: 07/08/2014, 18:21

... from healthy and HIV- infected subjects Interestingly, in HIV subjects, H37Rv infection induced the blood cultures to produce increased levels of pro-inflammatory cytokines such as IL-1, TNF-α, and ... effects of GSH in growth control of H37Rv in blood cultures derived from healthy and Figure HIV- infected subjects Model describing direct and indirect effects...

Ngày tải lên: 10/08/2014, 05:20

... designated HeLaTR /4-1BB and HeaLaTR/ TRAF1, respectively Primers for amplifying the 4-1BB and TRAF1 cDNAs were: 5¢-GAATTCAAGCTTATGGGA AACAGCTGTTACAACATA-3¢ and 5¢-GAATTCAAG CTTCACAGTTCACATCCTCCTTCTTCT-3¢ ... used for amplifying the hTRa1 cDNA were: 5¢-CCCGGGAAGCTTCGGACCATGG AACAGAAGCCAAGCAAGGTG-3¢ and 5¢-CCCGGG GTCGACGACTTCCTGATCCTCAAAGACCTC-3¢ In order to overexpress 4-1BB...

Ngày tải lên: 23/03/2014, 18:20

... loudspeakers and paging system, and a detailed log of all calls is maintained Criteria for medical emergency team activation Calling criteria for our MET service are based on acute changes in heart ... drugs and defibrillators Outcome measures Information on the activation of all MET calls is maintained on a hospital switchboard logbook that includes the date and time...

Ngày tải lên: 25/10/2012, 10:45

... et al MT1-MMP and furin can interact with GRASP55 Overexpression of GRASP55 has been found to increase the amount of complex containing MT1MMP and furin, and the expression of a catalytically inactive ... with a full-length furin cDNA, were permeabilized and stained with polyclonal antibodies against furin and GRASP55 Arrows show examples of membrane c...

Ngày tải lên: 18/02/2014, 04:20

... aprotinin, leupeptin and pepstatin, twice in kinase buffer, and used for in vitro kinase assays In vitro kinase assays Recombinant human protein kinase CK2 holoenzyme (0.5 U, corresponding to ... orthologues occur in higher eukaryotes) [13] Rio1 protein kinase is regulated by CK2 The biological role of Rio1p or even the pathways in which the Rio1 prote...

Ngày tải lên: 18/02/2014, 16:20

... that measurement of acid phosphatase activity was the best estimation of the mRNA abundance in our systems, and from here on we use the ratio of acid phosphatase activities as an indicator of gene ... tested the consistency of the GLAM ratios by performing the assay in a wide set of previously characterized mutants involved in mRNA biogenesis The GL...

Ngày tải lên: 07/03/2014, 12:20