Development of live bacterial delivery systems for presentation of dengue EDIII to the mucosal immune system 2

... 70 2. 2 .2. 3.1.3 Coomassie Blue staining 71 2. 2 .2. 3.1.4 Electroblotting 71 2. 2 .2. 3.1.5 Immunoblotting 71 2. 2 .2. 3 .2 Flow cytometry 72 2 .2. 2.4 Animal work 73 2. 2 .2. 4.1 Mouse strains 73 2. 2 .2. 4 .2 Intranasal ... Bacterial strains 67 2. 2 .2. 1 .2 Culture conditions 68 2. 2 .2. 2 Bacterial transformation 69 2. 2 .2. 2.1 Preparation of electrocompetent cells 6...

Ngày tải lên: 09/09/2015, 08:18

... the surface of PLGA NPs [62] These polymers have been designated as feasible candidates for drug delivery systems, anti -cancer agents and vaccine immunotherapy For example, DNA vaccine delivery ... doi:10.1186/1476-4598-10-3 Cite this article as: Bolhassani et al.: Improvement of different vaccine delivery systems for cancer therapy Molecular Cancer 2011 1...

Ngày tải lên: 06/03/2014, 02:21

... cells transformed with construct pMAL-c2x:NP Expression and purification of LASV NP from E coli Rosetta Expression and purification of LASV NP from E coli Rosetta 2(DE3) cells transformed with ... transformed with construct from E coli Rosetta gami cellsand purification of LASV GP2pMAL-c2x:GP2 Expression Expression and purification of LASV GP2 from E coli Rosetta g...

Ngày tải lên: 20/06/2014, 01:20

... thinking Because of the synthesis impairment, acetylcholine is decreased in the neurons in the Alzheimer disease brain, leading to the incapability of learning, memory, and thinking Providing ... there are great possibilities for them to be used in the construction of drug delivery systems in not too long a time The functionalization of CNTs used in the...

Ngày tải lên: 20/06/2014, 22:20

... densities up to 3.5 Tb/in2 [43] 1.7 Heat- Assisted Magnetic Recording The concept of heat- assisted magnetic recording (HAMR) is similar to the concept of magneto-optical recording proposed by Katayama ... by thermal fluctuations but is prevented by an energy 11 barrier given by the product of the magneto crystalline energy and the volume of the magnetic...

Ngày tải lên: 09/09/2015, 08:17

... serve as effective protein delivery systems for the repair 28 of bone tissue The merit of the work was that the PCL-based scaffolds could serve not only as a template for bone growth but also as ... fabrication The evaluation of these novel scaffolds forms the basis of this thesis It is hypothesized that PCL-TCP scaffolds would surpass the fir...

Ngày tải lên: 15/09/2015, 17:10

... overview of the progress made by using different types of promoters and vectors for gene delivery to the CNS 1.1 Current progress in gene therapy Gene therapy, which usually refers to somatic gene therapy, ... by the selection of promoters The gene delivery vector that carries and transfers the gene into the nuclei of target cell is another...

Ngày tải lên: 16/09/2015, 08:30

... center The New Town includes three components: Bac Linh Dam (North of Linh Dam) , Linh Dam peninsula in the middle and Linh Dam expansion (South of Linh Dam) The success of the project has set the ... south-west of Linh Dam lake with higher density of construction 665 Linh Dam new town - Solution for the High-density devel...

Ngày tải lên: 29/08/2013, 08:15

... nặng nhọc với mức thu nhập thấp, kéo theo điều kiện sống mức tối thiểu, tạm bợ khu nhà trọ rẻ tiền với điều kiện sinh hoạt an ninh không đảm bảo Đời sống tinh thần lao động hạn chế Họ thấy cô ... có xu hướng linh hoạt phụ thuộc nhiều vào mặt hàng họ bán “độ đắt hàng” Trung bình họ làm việc 13,09 giờ/ngày lúc bắt đầu công việc từ 2h30 - 3h00 sáng mặt hàng họ bán rau, hoa, quả… - 7h s...

Ngày tải lên: 29/08/2013, 08:15

... served as cold or hot beverages Homemade chicken soup has always been perceived to be healthful.28 Before the concept of nutraceuticals was introduced, chicken soup competed with wine and yogurt for ... can be formulated to produce nutraceutical drinks is unlimited Sport beverages have a special purpose and for that reason may not be thought of as Þrst-line nutraceutical bevera...

Ngày tải lên: 25/10/2013, 21:20

... 15% 15% c inadequate to improve reading skills: 40% d monotonous: 30% 40% > + ) $ $ B a various: 13% 13% 29% b useful: 12% 12% c inadequate to improve reading skills: 46% d monotonous: 29% 46% ... c difficult: 10% d relevant: 25% e others: 10% 25% 40% 10% ) > 23% ) 0% 9% a interesting: 9% b boring: 48% c difficult: 20% d relevant: 23% 20% )&> 48% e others: 0% ) A , ) / / * 9A!R<...

Ngày tải lên: 29/01/2014, 14:39

... participation of the private sector For Vietnam, the context in which the participation of the private sector is in very faint role, infrastructure investment finance is mainly depends on ODA funds Efficiency ... Conclusions: The study demonstrate the from the accessibility of the private sector to the ODA funds for infrastructure developm...

Ngày tải lên: 17/03/2015, 12:53

... well-defined, low Mw chitosans (chitosan oligomers) as delivery systems for nucleic acids (pDNA and siRNA) in vitro and after lung administration in vivo In addition, the concept of receptor targeting ... uptake and efficient endosomal release Besides their efficiency as pDNA delivery systems, lipid-based formulations were reported as efficient delivery...

Ngày tải lên: 23/08/2015, 17:29

... nanoparticulated combinatorial drug delivery system for simultaneous antiangiogenesis and anticancer activities could improve the therapeutic outcome The nanoparticle formulations described in this ... ligands for antitumor and antiangiogenesis activity (79-81) 1.3.2 Vascular targeted nanoparticle drug delivery Nanoparticulate delivery system surface modified with in...

Ngày tải lên: 11/09/2015, 09:58