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Development of live bacterial delivery systems for presentation of dengue EDIII to the mucosal immune system 2

Development of live bacterial delivery systems for presentation of dengue EDIII to the mucosal immune system 2

Development of live bacterial delivery systems for presentation of dengue EDIII to the mucosal immune system 2

... 70 2. 2 .2. 3.1.3 Coomassie Blue staining 71 2. 2 .2. 3.1.4 Electroblotting 71 2. 2 .2. 3.1.5 Immunoblotting 71 2. 2 .2. 3 .2 Flow cytometry 72 2 .2. 2.4 Animal work 73 2. 2 .2. 4.1 Mouse strains 73 2. 2 .2. 4 .2 Intranasal ... Bacterial strains 67 2. 2 .2. 1 .2 Culture conditions 68 2. 2 .2. 2 Bacterial transformation 69 2. 2 .2. 2.1 Preparation of electrocompetent cells 69 2. 2 .2. 2 .2 Electroporation 69 2. 2 .2. 2.3 Double homologous ... response 21 1.5 LIVE BACTERIAL VECTORS FOR ANTIGEN DELIVERY 22 1.5.1 Rationale for using a live bacterial vector 22 1.5 .2 Attenuated pathogens 24 1.5 .2. 1 B pertussis as a live vector for nasal delivery...
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Improvement of different vaccine delivery systems for cancer therapy pptx

Improvement of different vaccine delivery systems for cancer therapy pptx

... the surface of PLGA NPs [62] These polymers have been designated as feasible candidates for drug delivery systems, anti -cancer agents and vaccine immunotherapy For example, DNA vaccine delivery ... doi:10.1186/1476-4598-10-3 Cite this article as: Bolhassani et al.: Improvement of different vaccine delivery systems for cancer therapy Molecular Cancer 2011 10:3 ... consisting of two doses of DNA with EP [10] Actually, this approach might be very attractive because it would eliminate the need for two different types of vaccine For example, the use of a DNA vaccine...
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Báo cáo hóa học:

Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc

... cells transformed with construct pMAL-c2x:NP Expression and purification of LASV NP from E coli Rosetta Expression and purification of LASV NP from E coli Rosetta 2(DE3) cells transformed with ... transformed with construct from E coli Rosetta gami cellsand purification of LASV GP2pMAL-c2x:GP2 Expression Expression and purification of LASV GP2 from E coli Rosetta gami cells transformed ... Gami bla Figure strategy for expression of LASV proteins GP1, GP2, and NP in E coli using pMAL vectors Cloning Cloning strategy for expression of LASV proteins GP1, GP2, and NP in E coli using...
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Báo cáo hóa học:

Báo cáo hóa học: " The application of carbon nanotubes in target drug delivery systems for cancer therapies" docx

... thinking Because of the synthesis impairment, acetylcholine is decreased in the neurons in the Alzheimer disease brain, leading to the incapability of learning, memory, and thinking Providing ... there are great possibilities for them to be used in the construction of drug delivery systems in not too long a time The functionalization of CNTs used in the construction of drug delivery systems ... surge in the number of publications in this interesting field Apart from their uses in the cellular imaging with diagnostic effects in nanomedicine [4,5], CNTs are promising drug carriers in the target...
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THE STUDY OF NOVEL LIGHT DELIVERY SYSTEMS FOR HEAT ASSISTED MAGNETIC RECORDING

THE STUDY OF NOVEL LIGHT DELIVERY SYSTEMS FOR HEAT ASSISTED MAGNETIC RECORDING

... densities up to 3.5 Tb/in2 [43] 1.7 Heat- Assisted Magnetic Recording The concept of heat- assisted magnetic recording (HAMR) is similar to the concept of magneto-optical recording proposed by Katayama ... by thermal fluctuations but is prevented by an energy 11 barrier given by the product of the magneto crystalline energy and the volume of the magnetic grain The magnetic orientation of a magnetic ... to enhance magnetic recording 1.4 Conventional Recording Schemes 1.4.1 Longitudinal Magnetic Recording (LMR) In LMR, the information is written along the surface of the magnetic media The media...
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The evaluation of bioactive polycaprolactone scaffolds as protein delivery systems for bone engineering applications

The evaluation of bioactive polycaprolactone scaffolds as protein delivery systems for bone engineering applications

... serve as effective protein delivery systems for the repair 28 of bone tissue The merit of the work was that the PCL-based scaffolds could serve not only as a template for bone growth but also as ... fabrication The evaluation of these novel scaffolds forms the basis of this thesis It is hypothesized that PCL-TCP scaffolds would surpass the first generation PCL scaffolds as a graft material and protein ... evaluate the efficacy of the scaffolds as delivery systems for multiple growth factors, platelet-rich plasma (PRP) was used The buffers sufficed as important determinants of the release profiles...
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Development of hybrid promoters and viral vectors for improved gene delivery to the central nervous system

Development of hybrid promoters and viral vectors for improved gene delivery to the central nervous system

... overview of the progress made by using different types of promoters and vectors for gene delivery to the CNS 1.1 Current progress in gene therapy Gene therapy, which usually refers to somatic gene therapy, ... by the selection of promoters The gene delivery vector that carries and transfers the gene into the nuclei of target cell is another key Chapter Introduction issue of gene therapy The tropism of ... 2003) 1.4.2 Viral vectors Viral vectors are the most efficient vectors to transduce mammalian cells, and therefore are employed in most gene delivery experiments A variety of viral vectors, including...
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LINH DAM NEW TOWN - SOLUTION FOR THE HIGH-DENSITY DEVELOPMENT OF NEW SETTLEMENTS IN THE SOUTH-WEST OF HANOI

LINH DAM NEW TOWN - SOLUTION FOR THE HIGH-DENSITY DEVELOPMENT OF NEW SETTLEMENTS IN THE SOUTH-WEST OF HANOI

... center The New Town includes three components: Bac Linh Dam (North of Linh Dam) , Linh Dam peninsula in the middle and Linh Dam expansion (South of Linh Dam) The success of the project has set the ... south-west of Linh Dam lake with higher density of construction 665 Linh Dam new town - Solution for the High-density development of New settlements in the South-West of Hanoi Figure Land use pattern of ... street) in Linh Dam (Mapping - Quyen Phuong, 2011) 669 Linh Dam new town - Solution for the High-density development of New settlements in the South-West of Hanoi back to previous planning projects...
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LINH DAM NEW TOWN - SOLUTION FOR THE HIGH-DENSITY DEVELOPMENT OF NEW SETTLEMENTS IN THE SOUTH-WEST OF HANOI

LINH DAM NEW TOWN - SOLUTION FOR THE HIGH-DENSITY DEVELOPMENT OF NEW SETTLEMENTS IN THE SOUTH-WEST OF HANOI

... nặng nhọc với mức thu nhập thấp, kéo theo điều kiện sống mức tối thiểu, tạm bợ khu nhà trọ rẻ tiền với điều kiện sinh hoạt an ninh không đảm bảo Đời sống tinh thần lao động hạn chế Họ thấy cô ... có xu hướng linh hoạt phụ thuộc nhiều vào mặt hàng họ bán “độ đắt hàng” Trung bình họ làm việc 13,09 giờ/ngày lúc bắt đầu công việc từ 2h30 - 3h00 sáng mặt hàng họ bán rau, hoa, quả… - 7h sáng ... làm việc chợ lao động thuê trọ theo ngày (đêm), lại thuê nhà theo tháng Có khoảng 22,86% nữ lao động tự phải sống nơi có điều kiện sinh hoạt không đảm bảo, vệ sinh; 40,95% không sử dụng nước...
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Beverages as Delivery Systems for Nutraceuticals

Beverages as Delivery Systems for Nutraceuticals

... served as cold or hot beverages Homemade chicken soup has always been perceived to be healthful.28 Before the concept of nutraceuticals was introduced, chicken soup competed with wine and yogurt for ... can be formulated to produce nutraceutical drinks is unlimited Sport beverages have a special purpose and for that reason may not be thought of as Þrst-line nutraceutical beverages Sport beverages ... be a prebiotic for use in beverages 132 No single carbohydrate or potential source of dietary Þber has generated as much interest in the past to years as FOS Commonly referred to as a prebiotic,126...
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Development of supplementary materials to improve reading skills for the first year english majors at military science academy

Development of supplementary materials to improve reading skills for the first year english majors at military science academy

... 15% 15% c inadequate to improve reading skills: 40% d monotonous: 30% 40% > + ) $ $ B a various: 13% 13% 29% b useful: 12% 12% c inadequate to improve reading skills: 46% d monotonous: 29% 46% ... c difficult: 10% d relevant: 25% e others: 10% 25% 40% 10% ) > 23% ) 0% 9% a interesting: 9% b boring: 48% c difficult: 20% d relevant: 23% 20% )&> 48% e others: 0% ) A , ) / / * 9A!R< 9A?R< ... 6R< + 9A!R< 9!R< ) :A B, $ ) / ? ) ) * # * !6 9?: R< / $ $ : = ;> ? ( 20% a at home: 80% b in class: 20% 80% + + 15% a at home: 85,5% b in class: 14,5% 85% +& & + ) ) , ?6 6R / / / ) ) * / ) ?!R...
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Accessibility of private sector to the official development assistance funds for infrastructures development in Vietnam

Accessibility of private sector to the official development assistance funds for infrastructures development in Vietnam

... participation of the private sector For Vietnam, the context in which the participation of the private sector is in very faint role, infrastructure investment finance is mainly depends on ODA funds Efficiency ... Conclusions: The study demonstrate the from the accessibility of the private sector to the ODA funds for infrastructure development projects There are four main reasons for ODA shift from the public to the ... works for the consulting company under the private sector implements the ongoing projects but employees of the private sector are paid ten times the salary of public sector employees The lack of incentives,...
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Linear and branched chitosan oligomers as delivery systems for pDNA and siRNA in vitro and in vivo

Linear and branched chitosan oligomers as delivery systems for pDNA and siRNA in vitro and in vivo

... well-defined, low Mw chitosans (chitosan oligomers) as delivery systems for nucleic acids (pDNA and siRNA) in vitro and after lung administration in vivo In addition, the concept of receptor targeting ... uptake and efficient endosomal release Besides their efficiency as pDNA delivery systems, lipid-based formulations were reported as efficient delivery systems for siRNA in vitro and in vivo [105-107] ... conventional chitosans in vitro and in vivo (Paper I) Enhance the transfection efficiency of chitosan oligomer-based pDNA formulations in vitro and after lung administration in vivo by coupling a targeting...
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Drug delivery systems for antiangiogenesis and anticancer activities

Drug delivery systems for antiangiogenesis and anticancer activities

... nanoparticulated combinatorial drug delivery system for simultaneous antiangiogenesis and anticancer activities could improve the therapeutic outcome The nanoparticle formulations described in this ... ligands for antitumor and antiangiogenesis activity (79-81) 1.3.2 Vascular targeted nanoparticle drug delivery Nanoparticulate delivery system surface modified with integrin targeting ligand ... targeted nanocapsule with sequential drug delivery for temporal antiendothelial and anticancer activities 44 3.1 Introduction 45 III 3.2 Materials and methods 46 3.2.1...
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