adjuvants and delivery systems for induction of mucosal immunity

... DEVELOPMENT OF LIVE BACTERIAL SYSTEMS FOR PRESENTATION OF DENGUE EDIII TO THE MUCOSAL IMMUNE SYSTEM LAM JIAN HANG B.SC (HONS), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... infections begin [103] Therefore, the induction of mucosal immunity is beneficial in preventing the establishment of infection A second advantage of mucosal immunisation is its ease of administration There ... SUITABILITY OF EDIII AS A SUBUNIT VACCINE CANDIDATE 4.2 146 BORDETELLA PERTUSSIS AS A LIVE VECTOR FOR THE PRODUCTION AND MUCOSAL DELIVERY OF DENV2 EDIII 4.3 147 LACTOCOCCUS LACTIS AS A LIVE VECTOR FOR...

Ngày tải lên: 09/09/2015, 08:18

... outcome of immunization In designing vaccine, clearly regimens, plasmid dose, timing of doses, adjuvants, delivery systems and/ or routes of vaccination must be considered [11] Indeed, efforts to ... currently of considerable interest The success of intranasally delivered mucosal vaccines has been also limited by lack of effective vaccine formulations or delivery systems suitable for use in ... the environment prior to delivery and aid in targeting to a specific cell type for efficient delivery [1] Liposomes and polymers have also been utilized for the delivery of plasmid DNA, although...

Ngày tải lên: 06/03/2014, 02:21

... DM uses morphological information as well, DF uses frequency information and DMF uses morphological and frequency information We evaluated it for all words, and also for words with frequency ... number of zero frequency word types in We examined each cluster and chose a simple regular expression to describe it, and calculated the precision and recall for words of all frequency, and for ... secondly the string of characters that it is made of, and thirdly that it is a new word and therefore rare 7.1 Future work We have so far used only a limited form of morphological information that...

Ngày tải lên: 31/03/2014, 20:20

... FSH, LH, SB and EGF for 40 h; +U0126: COCs were cultured with FSH, LH and U0126 for 40 h; +U0126+EGF: COCs were cultured with FSH, LH, U0126 and EGF for 40 h lular mechanism of induction of the expression ... LH and H89 for 2.5 h; SB: COCs were cultured with FSH, LH and SB203580 for 2.5 h; LY: COCs were cultured with FSH, LH and LY294002 for 2.5 h; U0126: COCs were cultured with FSH, LH and U0126 for ... ng poly-dT and 0.25 U of avian myeloblastosis virusreverse transcriptase at 42°C for 75 and 95°C for PCR conditions were set as follows: cDNA was amplified for X cycles (Table 1) of denaturation...

Ngày tải lên: 20/06/2014, 07:20

... http://www.nanoscalereslett.com/content/6/1/555 Page of 22 Figure The formation of SWCNT and its physical and chemical treatment for use as drug carriers (A) The schematic illustration of the structure formation of SWCNTs with the ... tumor formation is the results of the gene alterations and gene therapy aims to correct them For all of the gene-based Page 13 of 22 therapeutic strategies, efficient and safe gene delivery systems ... halogens, and the reactions often occur only in extreme conditions for the formation of covalent bonds Furthermore, the characterizatiuon of functionalized SWCNTs and the determination of the precise...

Ngày tải lên: 20/06/2014, 22:20

... interpretation and writing of manuscript 19 20 Acknowledgements We thank Lan Lin for help with preparation of manuscript and figure, and RK Yedavalli for assistance with reference formatting 21 ... caspase profiles in Jurkat and JPX-9 cells after Zn and Cd treatments (Figure 5A) Activation of caspases 3, 8, and requires the processing of pro-protein precursors to smaller active forms To ... untreated) 1.2 Figure Quantitation of apoptosis and viability in Jurkat and JPX-9 cells treated with ZnCl2 or CdCl2 Quantitation of apoptosis and viability in Jurkat and JPX-9 cells treated with ZnCl2...

Ngày tải lên: 13/08/2014, 13:20

... for the cellular delivery of nucleic acids have caused various problems for successful clinical gene therapy [15,16] 13 Gene delivery systems Viral gene delivery systems Viral gene delivery systems ... delivery systems for gene therapy of genetic or acquired human diseases has motivated the search for, and utilisation of, safer carrier molecules Non-viral delivery systems such as naked or formulated ... evidence for the potential of chitosan to improve the transmucosal delivery of macromolecular drugs The positive effect of chitosan solutions/powders on the transmucosal delivery of peptides and proteins...

Ngày tải lên: 23/08/2015, 17:29

... to the academic and research staff at the Department of Electrical and Computer Engineering, Spin and Energy Lab (SEL) and Centre for Optoelectronics (COE) ii Abstract The demand for magnetic storage ... antiquity, and one of the first widespread uses of magnets was as a navigational aid in the form of a compass In a bar magnet, the end of magnet pointing toward North is termed the north pole, and the ... Table of Contents Acknowledgements i Abstract iii Table of Contents vi List of Figures xi List of Tables xviii List of Symbols and Abbreviations...

Ngày tải lên: 09/09/2015, 08:17

... modification Briefly, 0.5 g of PLGA was dissolved in 10 ml of methylene chloride and activated by the addition of 26.3 mg of p-nitrophenyl chloroformate and 18 μL of pyridine to the solution, ... concentrations of equivalent PTX 54 XIII 3-4 Immunofluorescence staining of β-tubulin of HUVEC after treated by blank lipid formulation as control (a) and 2.5 μM CA4 equivalent lipid formulation ... PTX and CA4 78 4-8 Histology of tumor tissues with treatment of different formulations 79 4-9 Change of body weight during treatments 80 XIV 5-1 Synthesis and physicochemical characterization of...

Ngày tải lên: 11/09/2015, 09:58

... DIVIDE -AND- CONQUER STRATEGY FOR IDENTIFICATION OF STRUCTURAL SYSTEMS AND MOVING FORCES TRINH NGOC THANH B.Eng (HCMUT) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CIVIL ... structural systems such as high-rise buildings, long-span bridges and offshore platforms often require inspection and maintenance for purpose of sustainable and safe usage The state of these large ... Identified moving forces for 5% noise: (a) force 1; (b) force 2; (c) total force; simulated force: continuous line; identified force: dash line  Figure 5.4 Identified moving force for 10% noise:...

Ngày tải lên: 11/09/2015, 10:00

... submitted for the degree of Doctor of Philosophy in the Department of Oral & Maxillofacial Surgery at the National University of Singapore under the supervision of Associate Professor Ho Kee Hai and ... mother, Madam Pushpa Devi, for all her sacrifices, for always being there and for pushing her to strive for excellence She thanks her bosom friends for being so kind and understanding during this stressful ... protein delivery systems for bone regeneration and has the potential for clinical applications Table of Contents PREFACE i ACKNOWLEDGEMENTS iii SUMMARY iv TABLE OF CONTENTS vii LIST OF FIGURES...

Ngày tải lên: 15/09/2015, 17:10

... chemical and physical properties and sources Maltitol (2) Palantinose and tea polyphenol (1) Maltitol and palantinose and tea polyphenol (1) Maltitol and palantinose and erythritol and tea polyphenol ... vehicle of choice for delivery of any nutraceutical or supplement to make a beverage, drink, or cocktail Milk and juices also receive their share of added nutrients and nutraceuticals However, for ... National Academy of Sciences’ recommended 25 g per day for women and 35 g per day for men110 for the positive effects of dietary Þber on the incidence of cancer to be seen The availability of a greater...

Ngày tải lên: 25/10/2013, 21:20

... protection land and the production forest land had been planned for growing forests, so there is no need to take into consideration the means of cultivated slope soil) The total area of the convertible ... convertible land and low erosion potential level + Group of the convertible land is equivalent to the level of the erosion potential: consists of soil with erosion potential from 100-200 and has area of ... (28.3% of the convertible land) This group has average centralization level of convertible land and average erosion potential level + Group of the convertible land is equivalent to the level of the...

Ngày tải lên: 13/02/2014, 12:20

... GAGAS standards There are a total of 10 standards for Financial Audits and standards for Attestation Engagements The complete text of the standards is available in the Yellow Book See Chapter for ... Estimating systems and proposal preparation practices • Contract cost accounting practices − Systems for tracking and allocating labor cost − Systems for allocating non-labor direct costs − Systems for ... reliability of performance measures The following page provides a summary matrix of applicable standards for audits of Schedules of Indirect Costs AASHTO Uniform Audit and Accounting Guide for Transportation...

Ngày tải lên: 18/02/2014, 01:20

... mere management of the work of an individual project For the purpose of describing and analyzing negotiations in project sales and delivery, this report uses two important areas of established ... analyze negotiations in the context of project sales and delivery processes The body of this report first develops an understanding of the concept of negotiation and reviews the negotiation analysis ... rights and - 20 - obligations of each party, with associated payoffs It is important to note, however, that the payoffs at the time of the signing of the contract are expected payoffs, and are...

Ngày tải lên: 18/02/2014, 11:20

... sample for each group with examples of verb and noun usages is shown in (9), (10), and (11) respectively The above figures again reflect the asymmetry between deverbalisation of verbs and denominalisation ... theoretical and practical concerns via the design of the tagset In that case the final lexicon would be theoretically valid on the one hand, and the tagged corpus would be of practical use for NLP ... each tag consists of a letter code for the general classification (i.e noun, verb, etc.) of the word, and another for the sub-classification according to the particular context For example, when...

Ngày tải lên: 08/03/2014, 21:20

... Quantification of each cytokine (in ngÆmL)1 for IFN-c and in pgÆmL)1 for IL-12p70) was performed based on the standard curve in each assay Preparation of nuclear extract The peritoneal cells and RAW264.7 ... suppression of the LPS-induced production of NO and IFN-c indicates that the signals of LPS for the production of these mediators are transduced through CD14 Stimulatory effect of IFN-c and IL-12 ... the production of NO and cytokines in response to LPS and its mediators Fig Stimulation effect IFN-c and IL-12 + IL-18 on peritoneal cells of C3H/HeN and C3H/HeJ mice for production of NO Peritoneal...

Ngày tải lên: 17/03/2014, 10:20

... (decreased/increased) and fragmented (field crop, evergreen forest, fallow forest) as a result of both farmers who lost their lands and turned to clear-cut forest areas for upland rice cultivation, and private ... to commercial and market-oriented farms These transformed sometimes happens in improperly ways and induced to change in land use and land covers by despoilment of forest covers and traditional ... The lowland farms are located between 300 to 450 msl Results and Discussions • The result of interpretation of two images ETM+1999 and ETM+2004, it provided two land use maps of 1999 and 2004...

Ngày tải lên: 17/03/2014, 11:20

... Assessment of air quality and environmental damage, evaluation of abatement option for the control of pollution and development of Optimum control strategy in the form of an action plan Steps for preparation ... improvement of the quality of the air and the prevention, control and abatement of air pollution To plan and cause to be executed a nation-wide programme for the prevention, control and abatement of air ... Preparation of Action Plans for Control of Air Pollution Constitution of Working Groups and Development of Working Strategy for Action Plan Preparation Action Plan Constitution of Committees for Surveillance...

Ngày tải lên: 29/03/2014, 14:20

... currently the Director of the Institute for Optical Sciences and full professor in the Departments of Chemistry and Physics He is a Fellow of the Royal Society of Canada and the holder of the Canada Research ... Foundation and a Companion of the Energy Institute She was awarded the Melchett Medal of the Energy Institute in 2002 and the Eva Philbin Award of the Institute of Chemistry of Ireland in 2007 ... Tenne for providing information, James Bolton for his early input into some of the material in Chapters and 4, the staff of World Scientific Press who expertly drew many of the diagrams, and Lenore...

Ngày tải lên: 04/06/2014, 14:30