Characterization of novel anticoagulants from hematophagous arthropods

... pillar of support iv TABLE OF CONTENTS ACKNOWLEDGMENTS i TABLE OF CONTENTS v Summary viii List of Tables x List of Figures xii List of Abbreviations ... classification of extracted coding sequences (CDS) from the putative housekeeping class from the sialotranscriptome of R pulchellus 66 Table 9: Functional classification of proteins from the ... housekeeping clas...

Ngày tải lên: 09/09/2015, 08:13

... Discussion and Conclusion Chapter Identification and characterization of novel inhibitors on extrinsic tenase complex from Bungarus fasciatus (banded krait) Venom 3.1 Introduction 3.2 Materials and ... Figure 4.1: Synthetic gene sequences of Fasxiator Figure 4.2: Identification of novel anticoagulants from Bungarus fasciatus venom Figure 4.3: Effects o...

Ngày tải lên: 09/09/2015, 11:08

... N-Terminal sequencing of the kininogens was only successful from the 42-kDa band of wolffish kininogen (XLVQPGVLI…, Table 1) The major bands of both kininogens and also the 60-kDa band of cod kininogen ... biantennary and triantennary N-glycans, with extensive sialic acid O-acetylation Also O-glycosidic glycans were recovered from wolffish kininogen The permethylated O...

Ngày tải lên: 08/03/2014, 23:20

... Bibliography 195 Appendix 224 Publications 238 vi Summary Identification and characterization of novel proteins from a rare Australian elapid snake Drysdalia coronoides Partial transcriptome from ... venomous terrestrial snakes of the Elapidae family have undergone an extensive radiation in Australia [12] Elapid snake genus Drysdalia from southern Austr...

Ngày tải lên: 11/09/2015, 10:02

... terminus of the CRFR1 isoforms (Fig 1C) The predicted masses of the isoforms without/with V5 tag are as follows: CRFR1a (47.7/52 kDa), CRFR1e1 (10.8/ 15.1 kDa), CRFR1e2 (28.1/32.4 kDa), CRFR1f ... CRFR1 a, b, c and d isoforms differ in their ability to bind ligands and activate G proteins [10,16,25] CRFR1a is the most efficient in the stimulation of cAMP productio...

Ngày tải lên: 07/03/2014, 15:20

... analysis of the PAH gene in 51 unrelated HPA patients from Southern Italy In addition to the molecular epidemiology of PAH mutations, we characterized the functional properties of two novel mutations ... phenylalanine hydroxylase deciency in Southern Italy: a 96% detection rate with ten novel mutations Ann Hum Genet 71, 1 8519 3 10 Waters PJ (2003) How PA...

Ngày tải lên: 16/03/2014, 01:20

... Effect of KCl on the activity of salt-active acharan sulfate lyase d, salt-active acharan sulfate lyase II purified from Fr-s in Fig 1; m, heparin lyase II-1 (acharan sulfate lyase 1) purified from ... specificity of purified salt-active acharan sulfate lyase The purified salt-active acharan sulfate lyase degraded heparin and heparan sulfate as w...

Ngày tải lên: 23/03/2014, 21:20

... 5¢-GGCACTTAACCCATGGTACATGGACCTTGATATTGAC-3¢ 5¢-GTCAATATCAAGGTCCATGTACCATGGGTTAAGTGCC-3¢ 5¢-GGACCTTGATATTGACGGTCCAGATACC-3¢ 5¢-GGTATCTGGACCGTCAATATCAAGGTCC-3¢ 5¢-GGATTGAAGGGGGAAGATCAGGAGGTGC-3¢ 5¢-GCACCTCCTGATCTTCCCCCTTCAATCC-3¢ ... 11222–11228 11 Sun Y, Mansour M, Crack JA, Gass GL & MacRae TH (2004) Oligomerization, chaperone activity, and nuclear localization of p26, a small heat...

Ngày tải lên: 30/03/2014, 20:20

... activation of factors V and VIII by thrombin and then prevent these cofactors from forming prothrombinase and tenase complexes On the other hand, as shown in Fig 7, madanin and suppressed thrombin- induced ... salivary glands of H longicornis [24] According to this report, an extract of the salivary glands prolonged both APTT and PT, suggesting the presence o...

Ngày tải lên: 31/03/2014, 01:20

... purification and characterization of chondroitin ABC and AC lyases, particulary chondroitin AC lyase, from an anaerobic bacterium (B stercoris) in human intestine The substrate specificity and other ... suggest that chondroitin ABC and AC lyases have exolytic and endolytic action patterns These chondroitin ABC and AC lyases showed optimal ac...

Ngày tải lên: 31/03/2014, 23:20

... doi:10.1186/2191-0855-1-26 Cite this article as: Phan et al.: Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus AMB Express 2011 1:26 ... such as KM, Vmax, and Kcat Applicability of P excavatus proteases Serine proteases The inhibitory effect of PMSF towards all fracti...

Ngày tải lên: 20/06/2014, 23:20

... thermal treatment on IgE- binding activities of Blo t 92 and Blo t 21 4.2.2 Effect of thermal treatment on Blo t and Blo t 21 folding 94 4.2.3 Resistance of IgE- binding of Blo t and Blo t 21 to acid, ... 21 75 and Blo t 3.2.10 Quantitative end point cross-inhibition of Blo t 21 and 77 Blo t 3.2.11 Localization of Blo...

Ngày tải lên: 14/09/2015, 12:44

... 2006 FEBS 3239 Biochemical characterization of annexin B1 A Winter et al Fig Amino acid alignment of annexin B1 from C cellulosae (GenBank accession number AF147955), annexin B12 from Hydra vulgaris ... presence of calcium were carried out with the addition of mm CaCl2 in the buffer For investi- Biochemical characterization of annexin B1 gation of the...

Ngày tải lên: 07/03/2014, 12:20

... although, as V volvacea produces at least one other laccase isoform in addition to lac1 [1], this and possibly other laccase isoforms may be contributing to the total laccase activity detected at the ... primers The putative N-glycosylation site is boxed; *; stop codon The putative polyadenylation signals (TATAAA and CATAAA) are in white on a black background Ó FEBS 200...

Ngày tải lên: 07/03/2014, 15:20

... purification of the recombinant enzyme In order to construct an expression vector for Cl-idh, two oligonucleotides (sense, 5¢-AAAAACATATGGCAAGCA AATCGACCATCATCTACAC-3¢, and antisense, 5¢-AAA AAGGATCCCGGCTGAAAACCGGGCTGCATTA-3¢) ... dehydrogenase is known to predominantly catalyze the reduction of oxaloacetate to malate, and thereby lowering the possibilities of oxaloacetate ac...

Ngày tải lên: 08/03/2014, 10:20