Screening and evaluation of the anticancer potential of scorpion venoms and snake venom l amino acid oxidase in gastric cancer

... SCREENING AND EVALUATION OF THE ANTICANCER POTENTIAL OF SCORPION VENOMS AND SNAKE VENOM L- AMINO ACID OXIDASE IN GASTRIC CANCER DING JIAN (B.S.c) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... responsible for the anticancer activities of BHV and LAAO The novel findings shed light on the development of anticancer agents from sco...

Ngày tải lên: 09/09/2015, 08:13

... that the antibacterial protein purified from the skin mucus of S schlegeli (SSAP) is a 120-kDa glycoprotein, being a new member of the l-amino acid oxidase (LAO) Fig Purification of antibacterial protein, ... because of the scarcity of the protein of interest In this study therefore we isolated the antibacterial protein, SSAP, from the skin...

Ngày tải lên: 30/03/2014, 10:20

... antibacterial protein in the epidermal mucus of the flounder Platichthys stellatus This species, which has a rich covering of mucus on its body surface, inhabits brackish water at the mouths of ... the antibacterial component of PSEM is a protein Purification of antibacterial protein from PSEM Fig Antibacterial activity of PSEM against (A) Staphy...

Ngày tải lên: 29/03/2014, 08:20

... Mixed inhibition of L2 by NATA with initial competitive inhibition suggests that NATA binds at two different sites, of which the substrate- binding site has higher afnity Inhibition by NAT Inhibition ... stability However, the inhibition proles of the substrate analogs NATA, NAT and OAB in the presence of l-Phe as substrate indicated that the two isoforms...

Ngày tải lên: 07/03/2014, 05:20

... it was suggested that amine oxidases are enzymes of cellular amino acid catabolism, comprising potential candidates for a mechanism that catalyses nitrogen mineralization from amino acids at the ... organism In conclusion, in the present study, a novel cytotoxic protein was isolated from the death cap and characterized The physico-chemical, chemical and biological ch...

Ngày tải lên: 29/03/2014, 08:20

... Nanyang Technological University, Singapore (Silver Best Poster Award) vi THE MECHANISTIC STUDIES OF THE ANTICANCER POTENTIAL OF ARTESUNATE IN HUMAN CANCER CELLS Table of Contents DECLARATION ... ART induced autophagy in human cervical cancer HeLa cells evidenced by the increase of autophagic flux In the search of the mechanisms for ART-induced...

Ngày tải lên: 09/09/2015, 08:16

... the importance of PT in the mitochondrial apoptosis, because in the absence of the hallmarks of PT, including the loss of the inner membrane potential and the rupture of the outer membrane, the ... hypoxia), and they found that the type, strength and kinetics of the target gene profiles depend on the levels of p53 in the cell, the type of in...

Ngày tải lên: 14/09/2015, 14:11

... serine and glycine was reduced from 33% in the light to 25% in the dark (Fig 7A) Glutamine and asparagine were the major amino acids in the xylem sap in both the light (63% of the total amino acids) ... 3195 Amino acid metabolism and translocation M.-H Valadier et al Table Amino acid composition in leaves, and amino acid percentage ratio i...

Ngày tải lên: 18/02/2014, 18:20

... et al Staphylococcal nuclease refolding investigated Two point-mutated proteins, with a single base substitution of alanine for tryptophan (W14 0A) and alanine for lysine (K13 3A) , and two truncated ... both the wild-type protein and mutants E142O and K13 3A Thermal analysis of protein unfolding The DSC curves of the wild-type protein and the mutants K13 3A,...

Ngày tải lên: 20/02/2014, 01:20

... Local stability and key acidic amino acids in staphylococcal nuclease by the interactions of E75 with H121 and K97 It is believed that a small number of key amino acid segments play major roles ... 44% of E129G), respectively, lower DHcal values than the wild-type protein These outcomes indicate that little in uence is exerted by Gly 3969 Local stabilit...

Ngày tải lên: 07/03/2014, 21:20

... control at t ¼ for each peptide The percentage of degradation was calculated by comparing the area of the peaks of the intact peptide at t ¼ and t ¼ 60 Binding assays Binding assays were carried ... [33], the peptides containing a b-amino acid substitution in position have increased stability compared to the corresponding a- amino -acid- containing peptides...

Ngày tải lên: 08/03/2014, 08:20

... with the amino group and the N3 atom of the cytosine (Fig 1) This study uses site-directed mutagenesis to further explore the contribution of these amino acids interacting with the pyrimidine ring ... consisting of three domains, the CORE, the LID and the NMPbind [1] A characteristic of bacterial CMP kinases is an extension of the NMPbind domain by 40...

Ngày tải lên: 16/03/2014, 10:20

... larger than the maximal O2 concentration in solution) [6–9] These enzymes appear to ‘consume’ O2 without highaffinity binding Because of the scarcity of information concerning the reaction of flavoproteins ... in the oxidized form, and the spectrum of the reduced enzyme is observed only at the end of the observation time, i.e when the O2 concentration becom...

Ngày tải lên: 28/03/2014, 23:20

... of PrPC-induced cell drug resistance in gastric cancer cells PI3K/Akt is involved in the activation of P-gp by PrPC in gastric cancer To further investigate the underlying mechanism of PI3K/Akt- mediated ... significantly increased The results indicate that inhibition of the PI3K/Akt signaling pathway may lead to inhibition of the MDR indu...

Ngày tải lên: 07/03/2014, 03:20