... nonionicdetergents: 0.05% (v/v) Nonidet P-40 or Tween-20.4. Enzyme substrates: 2,2'-azino-bis(3-ethylbenzathiazaline 6-sulfonic acid) (ABTS) (1 mg/mL,A405nm), O-phenyldiamine (OPD) (1 mg/mL, A492nm), ... Histologically-based methods for detection of mucin,in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana,Totowa, NJ.2. Davies, R., Carlstedt, I. (2000) Isolation of large gel-forming ... mucins, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ.3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: ...