... protease, PrtA, from an insect pathogen Photorhabdus luminescens and development of a highly sensitive and specific substrate Judit Marokha´zi1, Nikolett Mihala2, Ferenc Hudecz2,3, Andra´s ... the development of such a substrate based on analysis of PrtA cleavage site specificity, and kinetic characterization of PrtAactivity on the new substrate. Results and DiscussionIdentification of ... normalized with the internal standard, and thedegree of cleavage was calculated from the reduction in thenormalized area of the substrate peaks. For the identifica-tion of PrtA cleavage site...