Hydrolyzed feather protein in aqua feed

... [88]. Ten proteins were detected as IgE-binding proteins and 8 proteins as IgG4-binding proteins, with high IgE de- tection rate and specificity. Among the IgE-binding proteins, the proteins with ... iso- lated soy protein has a protein digestibility-corrected amino acid score of 1.0, which is the same as that of casein and egg protein [28]. However, soy proteins contain low methi...

Ngày tải lên: 31/10/2012, 14:59

... lgÆmL )1 modified LDL (1 mg protein mL )1 , incuba- ted with 100 m M modifying agent or 50 lM EDTA, in NaCl ⁄ P i , pH 7.4, for 7 days at 37 °C) for up to 96 h in medium containing 10% lipoprotein-deficient serum ... the modified apolipoprotein B-100 (apoB) protein. Cholesterol and cholesteryl ester accumulation occurs within 24 h of exposure to glycated low-density lipoprotein, and increa...

Ngày tải lên: 19/02/2014, 02:20

... shown to detect Cyp4x1 in brain; quantitation of the Cyp4x1 protein in brain demonstrated  10 ng of Cyp4x1 protein mg )1 microsomal protein, showing that Cyp4x1 is a major brain P450. Immunohistochemical ... preincubated with purified recombinant Cyp4x1 protein, resulting in the ablation of detection of the 55-kDa protein in brain (Fig. 5C). This shows that the antisera specific...

Ngày tải lên: 19/02/2014, 07:20

... that the N-terminal domain is involved in protein protein interactions with other hydrophilic domains of neighboring integral membrane subunits, and interspecies interactions are incompatible. From ... -terminus are predicted to form a domain on the matrix side, and 36 form a domain extending into the intermembrane space. In the third mutant (V79-G35) one might expect a protein to be...

Ngày tải lên: 19/02/2014, 16:20

... of the proteins eluting in the void peak (fractions 30–40 in Fig. 1A), but without any prominent smaller peaks in the region where the type I AFPs elute in winter flounder plasma [13]. A single peak ... this protein was 16 283.8 Da as determined by MALDI-TOF MS (Fig. 3). Like the 17 kDa winter flounder and Ameri- can plaice AFPs, the amino acid composition of this protein was rich...

Ngày tải lên: 20/02/2014, 01:20

... stored in 1.5 mL Protein LoBind Tubes (Eppendorf, Hamburg, Germany) at )80 °C until in- gel digestion. In- gel digestion and peptide extraction Protein- containing gel pieces from copper-stained or zinc- imidazole-stained ... gel-separated proteins visualized by reversible negative staining using imidazole-zinc salts. Electropho- resis 20, 732–737. 38 Lee C, Levin A & Branton D (19...

Ngày tải lên: 07/03/2014, 02:20

... [10,20,21], including perilipin, ADRP, and the tail-interacting protein, TIP47, collectively known as the PAT protein family [22]. These PAT proteins lack long runs of hydrophobic amino acid residues ... both the mouse intestine and liver by administration of the PPARa agonist. The induction ratios in both tissues were greater than 20, and the induced protein level in the intestinal...

Ngày tải lên: 07/03/2014, 05:20

... recombinant protein cannot be obtained. The present study repre- sents the first report based on a recombinant protein retaining almost the same structure as that of the native protein in the ... materials in 10 min at 25 °C was evaluated by measuring the decrease in pro- tein amount remaining in the solution. Adsorption to (A) calcite in ASW, (B) glass in ASW, (C) benzoguana...

Ngày tải lên: 07/03/2014, 05:20

... Src–SH2 binding protein. We observed that MVP interacts with Src in mammalian cells and inhibits the activity of Src tyrosine kinase. Results Isolation of MVP as Src–SH2 interacting protein by GS–SH2 ... ERK activation in Src over- expressing cells. To our knowledge, this is the first report of MVP interact- ing with a protein tyrosine kinase involved in a distinct cell signalling p...

Ngày tải lên: 07/03/2014, 12:20

... CLAP with a phosphoprotein stain did not reveal phosphate additions to these proteins. Only lanes in the gel containing the known phosphoproteins ovalbumin, b-casein and pepsin gave a reaction. ... fasciclin I domains in CLAP with selected proteins in the protein database. The Conserved Domain Database of NCBI was screened with the protein coding sequence of clone 1 of CLAP and the...

Ngày tải lên: 07/03/2014, 16:20