Báo cáo y học: "Fast genomic μChIP-chip from 1,000 cells" ppt

... of a one-day μChIP assay that enables genome-wide surveys of epigenetic histone modifications from 1,000 cells using microarrays. Typically, reliable resolution of ChIP location analysis is ensured ... (μChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. μChIP-chip reliably reproduces data obtained by large-scale assay...

Ngày tải lên: 14/08/2014, 21:20

... splenocytes were protected from arthritis and lacked IgG1 antibodies against proteoglycan. Angyal and colleagues conclude that the T lymphocytes’ function in proteoglycan- induced arthritis is ... T lymphocytes into recipient mice. One group of mice receiving whole spleen cells was treated with FTY720, an agonist of the phospholipid sphingosine 1 phosphate, which sequesters lymphocyte...

Ngày tải lên: 12/08/2014, 12:20

... ben- efits offered by such a powerful instrument. Thus, pub- lished items will be freely accessible to all readers, their copyright will be retained by the authors and they will be deposited ... Central and other full-text repositories. A widely accessible journal of this type may become a key resource for the worldwide sarcoma com- munity, filling some of the gaps in medical literature that...

Ngày tải lên: 13/08/2014, 15:21

... useful strategy may be to develop compound drugs that target groups of genes simultaneously, to counteract undesired indi- Genome Biology 2008, 9:R58 http://genomebiology.com/2008/9/3/R58 Genome Biology ... repeatedly on randomly permuted survival data. In this way we could assess how many interactions would be found if the gene expression levels and survival times were completely unrelated....

Ngày tải lên: 14/08/2014, 08:20

... (R.)-lactyl-CoA dehydra- tase from Clostridium propionicum. Stereochem istry of the dehy- dration of (R)-2-hydroxybutyryl-CoA to croton yl-CoA. Eur. J. Biochem. 206, 547±552. 20. Kelley, L.A., ... sodium borohydride to yield a protein-bound 2-amino-5-hydroxyvaleryl r esidue [11]. In addition, it has been shown that nucleophiles such as methylamine or hydroxylamine can cleave enzyme-bound th...

Ngày tải lên: 17/03/2014, 17:20

... D.O. & Frasch, A.C. (1999) Enzymically inactive members of the trans-sialidase family from Trypanosoma cruzi display beta-galactose binding activity. Glycobiology 9, 581–587. 11. Cremona, M.L., ... cruzi enzyme indicate a crucial role for Y1 19 in binding the acceptor carbohydrate, since the single substitution Y S strongly affects the transfer/hydrolysis ratio towards a more efficient...

Ngày tải lên: 31/03/2014, 23:20

... statistics and frequency analysis. Audio recorded interviews with allergists were transcribed verbatim and analyzed using content analysis of grounded theory methodology. Results: Ninety-two food allergic ... were analy zed using descriptive statistics and frequency analysis. Qualitative questions from the questionnaire and transcribed physi- cian interviews were analyzed using content anal...

Ngày tải lên: 08/08/2014, 21:20

... and 800 mg hydroxyapatite/cm) provided by the system manufacturer. The tissue mineral density was calculated only for voxels exceeding the threshold (that is, only for voxels occupied by mineralized ... density, the X-ray attenuation of each voxel was converted to mineral density using a calibration curve that was generated from a scan of a set of five hydroxya- patite phantoms of known den...

Ngày tải lên: 09/08/2014, 01:22

... best-evidence synthesis (Table 3), these two RCTs lack evidence of efficacy for collagen hydrolysate. In vitro, type I or type II collagen hydrolysate dose-dependently increased type II collagen synthesis ... any validation of the questionnaire limits the relevance of this study. Collagen hydrolysate Collagen hydrolysate is produced by enzymatic digestion of gelatin, which itself is produced...

Ngày tải lên: 09/08/2014, 08:22

... Spengler Genome Biology 2010, 11:105 http://genomebiology.com/2010/11/2/105 Page 2 of 3 by resetting the epigenomic activation pattern, they facilitate further downstream, currently unknown, mecha- nism(s) ... reproductive longevity in C. elegans. Science 2009, 326:954-958. 5. Hall SE, Beverly M, Russ C, Nusbaum C, Sengupta P: A cellular memory of developmental history generates phenotypic...

Ngày tải lên: 09/08/2014, 20:21