Báo cáo y học: "Chromatin architecture and gene expression in Escherichia coli" pdf

... following 30 minutes of treatment with the gyrase inhibitor norfloxacin, and (6) cells carrying a mutation (GyrAD82G) in a gyrase gene, respectively. Circle (7) shows the location of protein coding sequences ... the cell cycle and contains few genes, and euchromatin, which, on the other hand, contains gene- rich regions and in some cases clusters of highly expressed genes. Jeon...

Ngày tải lên: 14/08/2014, 14:21

... changes in degenera- tion include altered matrix synthesis, including a decrease in glycosaminoglycan production and an increase in collagen type I within the NP, and upregulation of matrix-degrading enzymes ... gene expression in malig- nant carcinoma via TNF-R1 [25], and TNF-R1 but not TNF-R2 has been shown to be important in inflammatory responses involving IL-1β and...

Ngày tải lên: 09/08/2014, 14:20

... may alter gene expression patterns in postmortem human brains. Study of expression levels of 14 genes in human brain autopsy and biopsy samples found significant change in one of the genes, indicating ... organization and biogenesis' and 'intra- cellular protein transport' (Table 2). The expression of genes involved in the ubiquitin cycle and protein ubi...

Ngày tải lên: 14/08/2014, 16:20

... the maximally expressed gene set and the more stringent SCS gene set, both in their ER (with both the mammalian and human estimators) and in gene age. Evolutionary rate, gene age and gene expression in ... gene we actually refer to the ER of its corresponding protein.) It is affected by many systemic factors, including gene dispensability, expression level, the...

Ngày tải lên: 14/08/2014, 21:20

... com- plexes may represent true physiologically relevant interactions in stationarily grown cells. Identification of proteins X (GatY) and Y (UP12) In order to identify polypeptides X and Y, we repeated ... °C for 8 min. Typically, cell extracts (5–10 lgof protein) were separated by SDS/PAGE using the standard Laemmli system [30] with 13% and 4% (w/w) acrylamide in the separating...

Ngày tải lên: 22/02/2014, 07:20

... cycle enzyme, whereas AcnA is an anaerobic stationary-phase enzyme which is specifically induced by iron and redox stress [29]. Interestingly, two enzymes are involved in the conversion of methylcitrate ... used, containing plasmids with the corresponding genes and an N-terminal cloned histidine tag. For purification of wild-type enzymes and expression studies, cells were grown aerobi...

Ngày tải lên: 17/03/2014, 10:20

... patients and 12 controls [18]. The microarray assay used (Panorama Cytokine Gene Array membranes; Sigma Genosys, Inc) included 375 genes enriched in cytokines, chemokines, cell surface 286 ably derive ... disintegrin and metalloproteinase domain 9 1.1 (α/β and γ→< 0) Up SERPING1 Serine (or cysteine) proteinase inhibitor (C1 inhibitor) 0.85 Up Up USP20 Ubiquitin specific protease...

Ngày tải lên: 09/08/2014, 01:23

... their own pro-inflammatory cytokines, including IL-1β and tumor necrosis factor-α, under the influence of proinflammatory cytokines present in the synovial tissues of patients with early OA [23,24], ... expanded in primary culture for 10 to 12 days and the gene expression of type I and II collagen (CI and CII, respectively), aggrecan (AGG), IL-1β and activin-like kinase (AL...

Ngày tải lên: 09/08/2014, 10:20

... are engineered to contain genes surrounded by loxP sites, resulting in a recombinant strain of mouse that eliminates a gene s expression only in the cells that express the Cre. These lines will ... with electrophysiology and neurotransmitter immunocyto- chemistry to describe the variability within and between 12 known classes of forebrain neurons in mice. Data such as these will...

Ngày tải lên: 14/08/2014, 07:21

... other indications of immune activation emerged in the set of over-expressed genes, including pro- inflammatory cytokines, chemokines, granzymes, protein- ases, and receptors for inflammatory mediators ... proliferative phenotype in circulating leu- kocytes from socially isolated individuals by identifying over- representation of Gene Ontology (GO) annotations involving immune response...

Ngày tải lên: 14/08/2014, 08:20