mechanisms the lymphocyte macrophage network and gene expression in sarcoidosis

... ABPs in transcription regulation B Zheng et al Table Role of nuclear actin-binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand-binding domain Actin-binding ... Bundling proteins Coactivator Supervillin NLS F-actin- and membraneassociated scaffolding protein Filamin NLS? Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin ()) Cross-linking ... occurring C-terminal 100 kDa fragment of filamin, interacting with the motor protein dynein, may exert its inhibitory effect by interfering with interactions between the N- and C-terminal domains, and...

Ngày tải lên: 07/03/2014, 01:20

... transforming the intensities, replacing missing values and collapsing probes to genes, the gene lists were filtered by minimum standard deviation = 0.7 Finally, the genes in the list 'pre-processing ... through the endothelial lining and into the SG [33] The reduced gene expression of Ifnz is of interest because the gene lies in the Nss1 congenic region The gene product is known to be inhibitory ... between the strains Gene expression differences of genes located in the NOD loci Idd5 and Nss1 The main focus of the gene expression profiling has been on identifying genes in the two NOD derived...

Ngày tải lên: 09/08/2014, 10:21

... cloning, with certain restrictions The second, currently seen in the Netherlands, permits the generation and use of hESCs but forbids therapeutic cloning The third, seen in Germany, forbids the generation ... gametes viz., eggs and sperm The canonical definition of a stem cell is a cell with the ability to divide indefinitely in culture and in the living organism whilst retaining the potential to give ... new organism thereby ensuring the continuation of the germ line In fact the zygote is at the top of the hierarchical stem cell tree being the most primitive and producing the first two cells by...

Ngày tải lên: 16/09/2015, 15:55

... also increased in mBAT in response to PM2.5 exposure We did not observe significant differences in the gene expressions of Hoxc9 or Igfbp3 in the WAT, neither was the gene expression of Dpt in ... antibodies After the PBS rinsing, the stain was developed using Fast 3, 3’-diaminobenzidine tablet sets (D4293; Sigma, St Louis, MO) The sections were then counterstained with hematoxylin and analyzed ... standard chow diet It is used for studies of hyperlipidemia and atherosclerosis, and has been used extensively in understanding the mechanisms of lipoprotein metabolism and atherosclerosis The...

Ngày tải lên: 06/03/2014, 19:20

... [18,30,31], including the bHLH-interaction and calmodulin-binding motifs in the DNA-binding domain [28,29], and the repression domain pdLNLD/ELxiG/S in the C-terminus [7] In our study, the spruce ... amino acid motifs in the variable C-terminal region, of which nine were previously unreported The amino acid motifs in the DBD and in the C-terminal region are useful to better characterise the ... transactivate some of the phenylpropanoid and anthocyanin genes through protein-protein interactions [32] The G motif in the Cterminus has been linked to transcriptional repression of the cinnamate 4-hydroxylase...

Ngày tải lên: 12/08/2014, 05:20

Ngày tải lên: 12/08/2014, 18:22

... the gyrase genes These genes are known to be sensitive to supercoiling and are involved in maintaining a precise level of supercoiling in the cell Thus, the inhibition of these proteins is very ... (M), counting from the beginning of the GenBank sequence more highly expressed genes are localized near the replication origins and the region around the replication terminus contains few highly ... (such as melting of the DNA helix) Thus, if there were regions along the chromosome that contained lots of binding sites for proteins involved in chromatin structure, most of the supercoiling would...

Ngày tải lên: 14/08/2014, 14:21

... upregulated in both hES cells and EB, node B contained the genes upregulated in hES cells only, node C contained the genes upregulated in EB only, and node D contained the genes that were upregulated in ... ACVR2B [32-35] The node containing genes upregulated in EB (node C) included the genes HAND1, HOXA1, HOXB2, MSX1, MSX2, MEIS1, FGF9 and FREM1 which are involved in morphogenesis and development ... Hierarchical clustering analysis of these genes also divided the samples into three groups, hES, EB, and adult cells, and divided the genes into major nodes (Figure 4) The node containing 226 genes that...

Ngày tải lên: 18/06/2014, 15:20

... to include the effect of TNF-α on matrix and MMP gene expression in normal and degenerate NP cells as well as the effect of IL-1β and TNF-α on cytokine gene expression and the expression of the ... MMP-9 gene expression in malignant carcinoma via TNF-R1 [25], and TNF-R1 but not TNF-R2 has been shown to be important in inflammatory responses involving IL-1β and MMP-3 and -9 in murine brain injury ... directly and by gene therapy inhibits matrix degradation in the intact degenerate human intervertebral disc: an in situ zymographic and gene therapy study Arthritis Res Ther 2007, 9:R83 Hoyland JA,...

Ngày tải lên: 09/08/2014, 14:20

... activity in diabetic mice, but not in non diabetic mice [46] In our study, curcumin and insulin increased the dopamine D1 receptor expression levels in the cerebellum, which suggests that the curcumin ... receptor binding parameters were determined using Scatchard analysis [32] The specific binding was deter- Page of 11 mined by subtracting non-specific binding from the total The binding parameters, ... to determine the fold change in expression This was done by normalizing the resulting threshold cycle (CT) values of the target mRNAs to the CT values of the internal control β-actin in the same...

Ngày tải lên: 10/08/2014, 05:21

... determine whether differences in expression levels of each gene existed between the spleen and liver These organs contain macrophages and other cell types capable of responding to LPS and other inflammatory ... least in part, for the increased cytokine and COX-2 gene expression observed in the spleen when compared to the liver Figure mRNA expression levels in parthenolide and LPS co-treatment spleen and ... parthenolide of proinflammatory gene expression reported in vitro (4–12, 15) and the small responses reported in animals here and previously (13) It is possible that in the whole animal the kinetics...

Ngày tải lên: 11/08/2014, 08:21

... significant in the inhibitory effect of Bee venom on the LPS-induced, and in the inhibitory effect of melittin on the SNP-induced NF-κB in THP-1 cells by 10 μM of SP600125 JNK inhibitor suppressed the inhibitory ... melittin and bee venom on iNOS and COX-2 expression, and on NO and PGE2 generation To investigate whether the suppressed effect of SP600125 on the inhibitory effect of bee venom and melittin on the ... melittin on the inflammatory gene expression, iNOS and COX-2 expression was determined The inhibitory effect of melittin and bee venom on iNOS and COX-2 expression by LPS and SNP in Raw 264.7 cells...

Ngày tải lên: 11/08/2014, 08:22

... zoledronate and clodronate did not significantly influence the gene expression of cyclin D1 during the first days Zoledronate, however, caused a decreasing cyclin D1 gene expression after the 6th ... Therefore, the genes of cyclin D1 and collagen were quantified by Real Time RT-PCR The nitrogen-containing bisphosphonates zoledronate and ibandronate were compared to the non-nitrogen-containing ... appeared to induce collagen type I gene expression during the first 10 days At day 14 the collagen gene expression level was lowered by the nitrogen containing bisphosphonates below 30% These results...

Ngày tải lên: 11/08/2014, 20:20

... binding protein A9 [S100A9], etc.) other "later" gene interactions were surprising (Figures and 5) Perhaps the most intriguing finding regarding GMA-MS welding fume Page of 18 exposure was the ... provide unique insight into strain- and welding fume-dependent genetic factors involved in the mouse lung response to welding fume The gene regulation and network interconnectivity reported in this ... 16 weeks in the B6 strain intriguing [29] The dysregulation of the calgranulins S100A8 and A9, in addition to CCL2 and IL8Rβ, warrants further investigation into a possible delayed inflammatory,...

Ngày tải lên: 12/08/2014, 11:22

... β-catenin initially in the vegetal micromeres (at the fourth cleavage) and subsequently in the macromeres and their progenitor blastomeres veg2 and part of veg1 The nuclearization of βcatenin in the ... plate, forming a ring of expression around both poles of the early embryo The sox4 gene, on the other hand, is expressed in the early blastula in the vegetal plate and is activated during gastrulation ... all, either in control or in treated embryos Also see Tables and and the text for further detail majority of genes expressed in the vegetal plate are severely reduced in expression, indicating that...

Ngày tải lên: 14/08/2014, 07:21

... protein and its expression profile, and related this to the function of the protein The term 'phyletic age' used here describes an estimated point in time when a protein integrated into the mouse ... described in Materials and methods A schematic description of the expression profile determination and protein annotation as described in Materials and methods The numbers in the pie charts indicate the ... data file contains the phyletic assignments of the proteins used in the analysis Additional data file contains the sequences of the proteins used in the analysis of the assignments The sequences...

Ngày tải lên: 14/08/2014, 14:21

... discuss our findings in the following sections Protein disorder and gene expression On consideration of the gene expression levels for transcripts encoding disorder in proteins, two main trends emerge ... biopolymers (such as proteins) and that the transcripts encoding highly disordered proteins and the resulting protein product remain in the cell longer to carry out function The gene expression measurements ... complexes Some of the genes are found only in prokaryotes - these include the peptidoglycan-associated lipoprotein and the glycine cleavage Complex H protein [78] - whilst other genes exist in all taxo-...

Ngày tải lên: 14/08/2014, 21:20

... boiling in a microwave and then immediately poured into a glass Coplin jar The Coplin jar was then placed into the 100°C water bath and the slides were then placed in to the Coplin jar for 20 minutes ... examined to determine the total volume of the precursor The area per point was 0.01 mm² and was determined at 4X magnification When measuring brain volume, the area inside the ventricles was included ... embedding process by first incubating the embryos in 1:1 xylenes and paraplast at 56°C for one hour This was followed by a 20 minute incubation in paraplast and then an overnight incubation in paraplast...

Ngày tải lên: 24/08/2014, 12:33

... using array rotation (rot), classlabel swapping (cperm) and gene swapping (gswap); the first number denotes the number of subnetworks in the numerator of the subnetwork-level agreement and the ... a set S within the sorted list (B) Plot finding; it is computed by comparing of the running sum for S in the data set, including the location of the maximum null distributions for the NES enrichment ... 2011) The gene sets are mapped to a gene- regulatory network (GRN), thus forming induced subnetworks These induced subnetworks are modeled as Petri nets, where the ‘places’ are genes and the ‘transitions’...

Ngày tải lên: 09/09/2015, 10:15

... interleukin gene; λIg: the λ-chain of immunoglobulin gene; γIg: the γ-chain of immunoglobulin gene; IFN-ind: the interferon-inducible gene 6-16; IL4R: the interleukin receptor gene; TAP2: the transport ... immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin receptor gene, TAP2 – the transport protein gene, hnRNP A1 – the human ... lymphomas In this group of upregulated genes there were the a-myb oncogene, the interleukin receptor gene (IL4R), the gene of the transporter protein TAP2, the gene of protease calpain small subunit...

Ngày tải lên: 02/11/2012, 11:08