Báo cáo sinh học: "Characterization of porcine ENO3: genomic and cDNA structure, polymorphism and expression" potx

... we describe the cDNA cloning and the expression analysis of two transcripts of the porcine ENO3 gene. In addition, the genomic structure, polymorphism and association analysis of this gene were ... analysis. Characterization of porcine ENO3 571 that of other species. As in man and rat [13,15], porcine ENO3 gene spans a region of about 6 kb and contains 12 exo...

Ngày tải lên: 14/08/2014, 13:21

... predicted and an apparent molecular weight of A12L Kinetic analysis and pulse chase of A12L proteinFigure 3 Kinetic analysis and pulse chase of A12L protein. A. To determine kinetic analysis of proteolytic ... discovery of requirements to initiate and regu- late viral cleavage processing other than the consensus of cleavage residues, identification of novel AG/X cleavag...

Ngày tải lên: 18/06/2014, 18:20

... a member of the cluster of retroviral proteases and serves to proteolytically process P3. Previous work established the N- and C-terminal amino acid sequences of CP and RT, processing of RT by PR, and ... the family of ion peaks, gave a protein with molecular mass of 13,794 ± 4 Da (Figure 1). The lack of recovery or detection of proteins of the size of CP subunits...

Ngày tải lên: 19/06/2014, 08:20

... (M.m.) and of blood plasma (500×) or hand surface (1×) from Homo sapiens (H.s.) is shown and compared to the activity of 500 pg ribonuclease A from Bos taurus pancreas. Molecular weights of the ... D) of the mRNA. The densitograms of bound radioactivity for the highlighted lanes (0' and 20') of the gels (A and C) are shown in the graphs (B and D). PSL mea...

Ngày tải lên: 14/08/2014, 19:22

... 3 University of California Los Angeles Dental Research Institute and University of California Los Angeles School of Dentistry, Los Angeles, California 90095, USA and 4 Department of Immunology, ... effective, presumably because of more efficient formation of dou- ble strand siRNA from a hairpin precursor as opposed to kinetic reassociation of sense and antisense follow...

Ngày tải lên: 14/08/2014, 19:22

... Sigma-Aldrich and Carlo Erba (Milan, Italy), and were of the highest available grade. Acknowledgements Thanks are due to Prof G.L. Sottocasa and Dr Anto- nella Bandiera (University of Trieste) ... Àðk A =k 0 Þ=½Að1Þ where k A and k 0 are the inactivation rate constants either in the presence or in the absence of various concentrations of a ligand A, k 2 and k 1 are the rat...

Ngày tải lên: 20/02/2014, 01:20

... M 624 Triazole-ThDP Pyruvate BA Fig. 4. (A) Superposition of the homology model of the ScPPDC dimer (orange) and the template (KdcA, PDB: 2VGB, cyan). The left hand side of the figure shows several of the additional loops necessary ... reaction of each variant with PPA is lower than that of the wild-type enzyme and, with the exception of Q448W, K m values are increased and k...

Ngày tải lên: 06/03/2014, 00:21

... to 6 .1 (Fig. 3). Fractions e and f have at leas t one unique isoform e ach ( pI 5.9 for e and pI 6 .1 for f), while two bands of pI 5.95 and pI 6.0werecommontoeachofthemajorCLAPfractions, although ... IEF standards (Std) and buffer control gelruninparallel.Thenumbersat the right represent the pI values of the major bands in e and f, while the numbers at the left are the pI value...

Ngày tải lên: 07/03/2014, 16:20

... level activity (100 %) and compared with the activity of mutated enzyme StkPK42R (lane 2) and StkP in the presence 0.1 m M,1mM and 10 mM of staurosporine (lanes 3, 4, and 5, respectively), a ... (lanes 1 and 5), cytosolic fraction (lanes 2 and 6) and membrane fraction (3 and 7) of S. pneumoniae strains. Purified recombinant StkP was used as a control (lane 4). Arrows indi...

Ngày tải lên: 16/03/2014, 18:20

... of 45 was similar to previously published results [15]. Analysis of the most enriched fraction by SDS/ PAGE and sensitive silver staining revealed the presence of a range of bands. A faint band ... of protein in dilute solution in the presence of detergents and lipids. Anal. Biochem. 138, 141–143. 23. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly...

Ngày tải lên: 17/03/2014, 09:20