analysis of genes and genomes phần 2 doc

... suggested by Watson and Crick. The separation of the DNA strands of the parent helix (black) means that each strand can act as a template of the synthesis of a new strand of DNA (blue). This ... contain one old strand and one new strand 28 DNA: STRUCTURE AND FUNCTION 1 Double-stranded DNA 2 nm 10 nm 30 nm “Beads on a string” Cross-section of 30 nm fibre Mitotic chromosome...

Ngày tải lên: 14/08/2014, 11:21

... 2. 13). 10000 Size (bp) Distance migrated (mm) 8000 6000 5000 4000 3500 3000 25 00 20 00 1500 1000 750 500 25 0 10000 12. 00 8000 12. 85 6000 14.55 5000 16. 12 4000 18 .24 3500 19.74 3000 21 .86 25 00 23 .97 20 00 27 . 32 1500 31. 72 1000 38.79 750 43 .29 500 50 .25 25 0 59.35 − + Direction ... cloning in 19 72 (Jackson, Symons and Berg, 19 72) . 1 02 BASIC TECHNIQUES IN GE...

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... 2 2 2 Extension temperature ( ◦ C) 70–75 70–74 70–75 70–75 70–75 [Magnesium] (mM) 1–4 1–4 2 4 2 4 2 4 pH @ 25 ◦ C 7.0–7.5 7.0–7.5 8.0–9.0 7.0–7.5 7.0–7.5 [dNTP] (mM) 40 20 0 40 20 0 40 20 0 40 20 0 ... between 17 and 30 nucleotides in length, which flank the DNA target sequence that is to be copied. One of the primers is the same sequence as one strand of the DNA Analysis of...

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... Initiative, 20 00) Oryza sativa (rice) 4 .3 × 10 8 12 ∼50 000 (Yu et al., 20 02; Goff et al., 20 02) Mus musculus (mouse) 2. 5 × 10 9 21 22 000 (Mouse Genome Sequencing Consortium, 20 02) Homo sapiens (human) 2. 9 ... sapiens (human) 2. 9 × 10 9 23 ∼ 32 000 (Lander et al., 20 01; Venter et al., 20 01) 6.6 TWO-HYBRID SCREENING 22 7 • Membrane proteins. The traditional two-h...

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... of PCR 1 binds to the antisense strand of PCR 2, then DNA polymerase can produce a double-stranded version of the gene that contains the mutation. In practice, the products of PCR 1 and PCR 2 ... architecture of a typical expression vector is shown in Figure 8.1. Analysis of Genes and Genomes Richard J. Reece  20 04 John Wiley & Sons, Ltd ISBNs: 0-470-84379-9 (H...

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... I2 ESTs PLOD2 Tropomyosin ESTs ESTs A X L A X L HIF1 integrin, β1 B A C D E Melanoma Expressed gene 31 30 29 28 27 26 25 24 23 22 21 20 19 18 17 16 15 14 7 8 9 10 11 12 13 6 5 4 3 2 1 Figure 10.5. ... 8 NH C CH O NH C CH HN O CH 2 CH 2 N N N N Ni 2+ − O − O C C O O O − CH 2 CH 2 N CH C O CH 2 CH 2 CH 2 CH 2 NH CH 2 CH CH 2 O OH Protein Ni 2+ -nitriloacet...

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... be induced to reproduce Analysis of Genes and Genomes Richard J. Reece  20 04 John Wiley & Sons, Ltd ISBNs: 0-470-84379-9 (HB); 0-470-84380 -2 (PB) 328 POST-GENOME ANALYSIS 10 Gene 1 URA3 URA3 Ura + PCR ... relies on either the crossing of plants containing single transgenes, or the concurrent transfer of transgenes (Daniell and Dhingra, 20 02) . In the chloroplast,...

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... 120 , 22 13 22 24. Efstratiadis, A., Kafatos, F.C., Maxam, A.M. and Maniatis, T. (1976) Enzymatic in vitro synthesis of globin genes. Cell, 7, 27 9 28 8. Eide, D.J. (1998) The molecular biology of ... al., 20 02; Dai et al., 20 02) or both (Phelps et al., 20 02) alleles of GGTA1 have been produced. Some of the knock-out pigs are apparently healthy and further work will asse...

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... 26 0 TRP1, 143, 145, 22 2 Tumour suppressor genes, 315 Tus, 35 Two-dimensional gel electrophoresis, 27 5 Two-hybrid screening, 21 8, 22 4, 22 7 Two-step PCR mutagenesis, 24 2 UAS G , 22 2, 22 4 ung, 23 9 URA3, ... 23 9 URA3, 143, 145, 22 2 Uracil, 9 Uracil N-glycosylase, 23 9, 24 0 UV light, 20 7, 23 2 UV cross-linking, 100 Variable number tandem repeats (VNTR), 29 2 Varshav...

Ngày tải lên: 14/08/2014, 11:21