Báo cáo y học: "Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana" ppsx

... out the molecular, immunological, and other in vitro experiments, and participated in the design of the study. SWH participated in the design of the study and provided technical support for the ... bp from the start site to the stop codon and contained four introns. The lengths of the introns were between 52–69 bp and were located in the first half of...

Ngày tải lên: 13/08/2014, 13:22

... content was estimated by using the method of Bradford [25]. For analysis of enzyme kinetics AK assay was done at varying concentrations of ATP and AMP and the results were analysed by double reciprocal ... Mannheim). AK assay Enzyme activity was assayed in the forward direction by the coupled reactions system [24] adding different concentra- tions of ATP and AMP as the...

Ngày tải lên: 21/02/2014, 00:20

... both EF-hands of CR I–II (Fig. 6) but only to EF-hand I of Calb I– II. These glycine chemical shifts report the formation of a strong interaction between the glycine amide and a carboxylate side-chain. ... high sequence identity, Fig. 1, and many other residues display conservative changes. The region between residues 54 and 79 of CR I – II, encompassing the linker, heli...

Ngày tải lên: 22/02/2014, 07:20

... 2004 Molecular and functional characterization of novel CRFR1 isoforms from the skin Alexander Pisarchik and Andrzej Slominski Department of Pathology and Laboratory Medicine, University of Tennessee ... activation of PKA and PKC together. We attempted to separate these effects by the addition of PKA inhibitor (H89). Unfortunately, these compounds inhibited reporter...

Ngày tải lên: 07/03/2014, 15:20

... VBARP. Also, the use of unsynchronized cells in these analyses further con- firmed that the cytoplasmic distribution of VBARP is independent of the cycling stage of the cells. Identification of biological ... Effect of VBARP siRNA on cell viability was tested by the trypan blue exclusion assay and cell viability assay. The cell viability results of VBARP and contro...

Ngày tải lên: 07/03/2014, 21:20

... the S100 family, the S100 A12 [10]. Hofmann et al. [11] proved that S100 A12, and possibly other members of the S100 family, mediates the activation of a novel proinflammatory axis by binding to ... of the Ca 2+ signal [3]. Kligman & Hilt [3] described the structural features that determine whether a protein is a member of the S100 family. They have two EF-hands per...

Ngày tải lên: 08/03/2014, 22:20

... antifreeze activity (Fig. 2). Equimolar solutions of deglycosylated and untreated smelt AFP displayed similar faceted ice crystal morphology (Fig. 2). The antifreeze activity of the untreat- ed and d eglycosylated ... untreated and deglycosylated smelt AFP were digested with Glu-C and trypsin in the presence and a bsence of Ca 2+ . Both enzymes generated some proteolysis...

Ngày tải lên: 24/03/2014, 03:21

... anteriorly and posteriorly from the cell body. A fluid-filled excretory canal is sur- rounded by the cell cytoplasm. The basal pole of the cell faces either the pseudocoel or hypodermis whereas the ... comprised of three cells, the excretory cell, the duct cell and the pore cell [8]. Destruction of any of these cells by laser abla- tion causes the animal to swe...

Ngày tải lên: 30/03/2014, 03:20

... alcohol, 2-methylbutanol, cis-2-hexenol and benzyl alcohol (in the presence of acetyl-CoA); ethanol, isoamyl alcohol and benzyl alcohol (in the presence of propionyl-CoA, isoval- eryl-CoA, n-butyryl-CoA, ... as 2- and 3-methylbutyl alcohol (also named amyl and isoamyl alcohols). The position effect of the methyl group on activity was weak for acetyl-CoA and propionyl-C...

Ngày tải lên: 31/03/2014, 21:21

... at days 1 and 15) was added to the immunosuppressive therapy and its therapeutic effect was evaluated. In addition, the levels and function of regulatory T lymphocytes and the apoptosis of immune ... stained using the TUNEL technique, and analyzed by flow cytometry before (day 0), and at days 30 and 90 of Rituximab ther- apy. Data from all samples studied are sh...

Ngày tải lên: 09/08/2014, 08:22