Báo cáo y học: " Intracellular assembly and budding of the Murine Leukemia Virus in infected cells" pps

... [30-32]). Recently, the observation of HIV intraendosomal budding and the discovery of the impact of endosomal proteins sorting pathway in retroviral budding have lead to the Trojan exosome hypothesis ... 76(10):4679-4687. 4. Yuan B, Campbell S, Bacharach E, Rein A, Goff SP: Infectivity of Moloney murine leukemia virus defective in late assembly events is res...

Ngày tải lên: 13/08/2014, 09:21

... DNA-provirus. This property of Mo-MuLV may explain why only small subpopulations of gammaretroviruses produce recombinants. In this con- text, the differences in the frequencies of recombination between ... [15], may be a local transport of RNA transcribed in the same locus of chromosome from the nucleus to their destination in the cellular cytoplasm. In the cy...

Ngày tải lên: 13/08/2014, 13:20

... leaks in the air-handling and sys- tem components after assembly of the NBIES. Leaks detected (if any) were sealed and the system repeatedly retested to verify remediation. To improve safety and extend ... likely in humans: aerosol transmission, ingestion of undercooked contaminated infected poultry, transmission by large droplets and self-inoculation of the na...

Ngày tải lên: 12/08/2014, 04:20

... followed by synthesis is puzzling or in any case very difficult to analyse in terms of kinetics. From a functional point of view, if a reaction reverse of synthesis followed by synthesis using CDP-DAGs ... membrane and in addition to de novo synthesis, the plant enzyme is capable of catalysing the exchange of the inositol polar head for another inositol. Our data clearl...

Ngày tải lên: 22/02/2014, 04:20

... to the memory of the late Professor Alexander Girshovich who initiated and was actively involved in the beginning of this work. We wish to thank I. Gokhman for her help during the subcloning of ... extracts using anti-GroEL Ig. It has been previously shown that at the beginning of the stationary phase the rate of synthesis of heat-shock proteins increases conside...

Ngày tải lên: 22/02/2014, 07:20

... factors [3] and their biotechnological potential. Lipases have proven to be versatile enzymes in nonaqueous solvent systems in which they catalyze the synthesis of a variety of acylglycerols and specialized ... triacylglycerols comprised of both long and medium chain fatty acids has been investigated as a means of providing single substitutes for mixed acylglycerides in...

Ngày tải lên: 23/03/2014, 21:20

... to the N-terminus of the m ature maltose-binding protein. Binding of the fusion protein to the IMAC gel in buffer containing 50 m M imidazole indicates the presence of the C-terminal histidine ... we veri®ed the identity of the hA2aR fusion protein by speci®c radioligand binding (see below), N-terminal sequencing and amino-acid analysis. The sequence obtained, na...

Ngày tải lên: 24/03/2014, 00:21

... 1241 phosphorylated Kdo residue in addition to lipid A whereby the Kdo is phosphorylated either at position 4 or 5. There was some uncertainty in the beginning whether the Kdo-5P was the result of phosphate ... is the endotoxin of Gram- negative bacteria; (c) LPS is a major surface antigen leading to the induction of protective a ntibodies; and (d) the understanding...

Ngày tải lên: 31/03/2014, 21:21

... only in their carbohydrate moiety. Analysis of the carbohydrate structures revealed that the brain enzyme contains a glycostructure of the oligoman- nose/hybrid type. The occurrence of terminal ... Characterization of a thy- roliberin-degrading serum enzyme catalyzing the hydrolysis of thyroliberin at the pyroglutamyl-histidine bond. Eur. J. Biochem. 99, 239–246. 10. B...

Ngày tải lên: 31/03/2014, 21:21

... is mainly contained within the protein-coding message; 2 of these 8 cycle in- phase, and 6 display opposite-phase expression. For 5 of 24 SAPs in which only the antisense transcript cycles, the ... transcription,translation,aswellasRNA silencing and stability [2,13-15]. Extensive transcription of intergenic regions and the antis ense strands of hundreds of annotate...

Ngày tải lên: 09/08/2014, 20:21