assembly and turnover of the d1 protein
Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx
... counteracting ability of the osmolyte does not arise from the stabilization of the native state but arises primarily from the destabilization of the unfolded state of the protein in the presence of osmolyte ... 64 and 82% of the total FtsZ were pelleted in the absence and presence of 0.8 m TMAO, respectively Electron microscopy analysis of the FtsZ assembly reaction showed the formation of thicker and ... area of proteins and the reduction in the solventaccessible surface area produces a decrease in the ligand-binding ability of the protein [52] An unfavorable interaction between protein and osmolyte...
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Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt
... higher protein content in their latex; and (2) the presence of other cysteine proteinases or isoforms in the latex To investigate these two hypotheses, the protein concentration per milligram of ... for the first time, we are not aware of the frequency of tapping in other studies These and probably several other, as yet unknown, factors affecting the activity and concentration of latex proteins ... sequences of the proteins VXH-I, VXH-III, VXH-IVa and VXH-IVb are shown in Fig 6, and are compared with the known N-terminal sequences of the cysteine proteinases of C papaya and V cundinamarcensis The...
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Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
... from Hanes plots Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined by ... indicated in Fig 4B The absence of Mo(V)signals reveals the g2-component of FeSII It is noted that the g-factors of the FeSI and II signals of the Qor proteins from the wild-type strain and from P putida ... catalyse the synthesis and insertion of a cytidine dinucleotide cofactor into recombinant Qor as suggested by the release of CMP after hydrolysis of the enzyme, it appears that the assembly of intact...
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Báo cáo khoa học: "Expression and characterization of the UL31 protein from duck enteritis virus" pot
... basic protein, and thus the molecular mass of the protein reacted with the UL31 antiserum was consistent with that predicted These results indicate that the 35 kDa protein is the product of the ... egress of Alpha-, Beta-, and Grammherpesviruses [8,14,16-20] However, there is no report on the identification and characterization of the UL31 gene product of DEV In the present study, the UL31 ... threonine, and on tyrosine residues (Fig 1) Figure Prediction of Phosphorylation sites of deduced amid acids of the DEV UL31 protein Prediction of Phosphorylation sites of deduced amid acids of the...
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Báo cáo y học: " Intracellular assembly and budding of the Murine Leukemia Virus in infected cells" pps
... membrane fission event and the final pinching off of the budding virus [4-6] In the last few years, late domain sequences were found to direct the interaction between the Gag proteins and some cellular ... support the hypothesis that the virus hijacks the MVB production system to direct the budding and the release of virus particles [10] Recently, it was shown that HIV and MLV Gag polyproteins ... vicinity of the plasma membrane, they probably have been just released from the plasma membrane In the cells, very few labeled-Gag localized individually at the plasma membrane and most of the Gag proteins...
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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
... supercomplexes and the effect of the absence of intact complex I, but the results are too preliminary in this regard Heterologous expression of the ESSS-HA subunit The localization of the ESSS subunit in the ... panel) The signal from the 18 kDa subunit of complex I (NDUFB6) served as another identification of the complex at the position of the histochemical stain in the left panel Antisera against the SDHC ... revealed the existence of two additional subunits (bringing the total to 46), and one of these, the ESSS protein, is also encoded by an X-linked gene in humans and mouse [36] Most of these subunits...
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Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
... interpretation of the data suggests that the indicated basic region of ic3 of the D2 receptor and acidic region of the C-tail of the D1 receptor might be involved in the interactions between the two ... dopamine D1 and D2 receptor fusion proteins (D1 CFP and D2–YFP) in comparison with emission spectra of the cell popula- tions that co-express dopamine D1 receptor fusion protein (D1 CFP) and one of the ... colocalization in D1 ⁄ D1, D1 ⁄ D1MUT, D1 ⁄ D2 and D1 ⁄ D2R1 assays and partial colocalization in D1 ⁄ D2R2 assays D1 ⁄ D2R3 does not colocalize (B) HEK293 cells were cotransfected with D2–CFP and either...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... [9] The KIND domain in these proteins is localized to the N-terminal region, and their specific functional domains are located in the C-terminal region The C-lobe of protein kinases mediates the ... bacterially expressed proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the KIND family of proteins, only KIND2 binds to MAP2 Pull-down assay (PD) of the endogenous MAP2 from ... cloned into pGEX-4T-2 The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were...
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Tài liệu Báo cáo khoa học: Redox regulation of dimerization of the receptor proteintyrosine phosphatases RPTPa, LAR, RPTPl and CD45 pdf
... conditions The ectodomains of both monomers in the dimer are required for this effect, suggesting that the epitope tag of one monomer is masked by the ectodomain of the other monomer in the dimer, and ... wedge-like structures to the N-terminal side of the D1, similar to the inhibitory wedge in RPTPa However, the crystal structures of the intracellular domains of LAR [19] and CD45 [20] suggest that ... oxidation induced changes in the conformation of the cytoplasmic domains of these RPTPs These results suggest there is flexibility in the cytoplasmic domain of RPTPs, and are evidence against rigid...
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Tài liệu Báo cáo Y học: A Raman optical activity study of rheomorphism in caseins, synucleins and tau New insight into the structure and behaviour of natively unfolded proteins pot
... longer-chain (and nonglobular) versions of the structure of the Bowman±Birk inhibitor in Fig The X-ray crystal structure pi2 reveals that the /,w angles of most of the residues of the Bowman±Birk ... helical conformation There may also be some b strand in some of the proteins, especially b- and j-casein judging by the wellde®ned negative ROA bands in these proteins in the range » 1245 cm)1, ... pi2) of the soybean variant of this protein, together with its ROA spectrum measured earlier [19] The general appearance of the ROA spectrum is quite similar to those of the caseins, synucleins and...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... GroEL from 15 and 30 lg of the cell lysate without (lanes and 3) or in the presence of ATP (lanes and 4) was detected with anti-UP12 Ig Samples (3 and lg of total proteins) of the cell extract ... 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids long; they are acidic and presumably located in the ... anti-GroEL Ig [27] The high yield of the UP12-GroEL complex isolation and the ATP-mediated dissociation of the complex strongly support the suggestion that the two stress proteins GroEL and UP12 interact...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
... which anchor the protein in the membrane The position and function of the long C-terminal tail on the other hand remains a matter of controversy [10,11,13–15] According to one hypothesis, the hydrophilic ... description of recent advances in the understanding of the channel-, motor- and regulatory components of the Tic complex For reference, some of the available knowledge, including the proposed function of ... incoming preproteins Additionally, because the demand of the chloroplast is ‘sensed’ inside the organelle, the IEM is closest to the origin of the signal, and thus regulation at the Tic complex...
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Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf
... for assembly of the iron– sulfur units It is of interest therefore to know whether the folding of these two proteins around their iron–sulfur cluster occurs similarly in mitochondria and in the ... assembly machinery of yeast mitochondria can build the inorganic unit of this normally cytosolic protein Furthermore, members of the extra-mitochondrial assembly line of these cofactors, the so-called ... mitochondria and the other one is unknown The protein bands separated from purified aggregates on SDS ⁄ PAGE were cut and identified using MS The results indicated the presence of hIRP1, as expected, in band...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... 205 and 215 nm, indicating that Rv1399c is composed of 22% of a-helices, 25% of b-strand and 39% of random coil The catalytic triad is located at the bottom of a small ˚ ˚ pocket, 20 A length and ... experimentally Indeed, the presence of Arg213 along with the proximity of the charge relay Asp260 within the active site could be responsible for the modification of the catalytic properties of the catalytic ... by the amides of Ala163, Gly89 and Gly90, the latter’s being found in the HGGG consensus sequence motif characteristic of members of the HSL family [35] Our model shows that the architecture of...
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Báo cáo khoa học: Identification and expression of the first nonmammalian amyloid-b precursor-like protein APLP2 in the amphibian Xenopus laevis ppt
... present in the other members of the APP superfamily, such as the absence of the exon encoding the KPI domain and the lack of a second heparin-binding domain [3,4,13,26] Comparative analysis of the Xenopus ... in the appearance of the APLP1 and APLP2 gene families The fact that both mammals and Xenopus contain APP and APLP2 proteins suggests that the first gene duplication giving rise to the APP and ... sequence comparison between the Xenopus and mammalian APLP2 proteins (A) Alignment of the amino acid sequences of Xenopus APLP2-A/B, and human, mouse and rat APLP2 proteins The one letter amino acid...
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Báo cáo Y học: Holliday junction binding and processing by the RuvA protein of Mycoplasma pneumoniae ppt
... bound the junction Each formed two distinct complexes (Fig 2A) In the case of the E coli protein, the two complexes represent the binding of either a single tetramer of RuvA (complex I) or of two ... of the sequences forming the pin in the majority of bacteria with the exception of two Mycoplasma species and one of Ureaplasma In the RuvA proteins from these organisms the pin sequence is extended ... comparison of the binding of EcRuvA and MpRuvA to linear duplex DNA and shows the additional stability of the MpRuvA bound DNA complex compared to the EcRuvA bound DNA complex But the results...
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Báo cáo khoa học: Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida pptx
... to that of PMT-C3 is that, at the sequence level, there is no discernible similarity of PMT-C3 to the proteins, with the exception of the Cryseobacterium protein glutaminase However, there is ... cellular outcomes, the sequence and structure of the activity domain of PMT differ considerably from those of the other dermonecrotic toxins [3] and point to a clear functional example of convergent ... this review, we focus on PMT and our current understanding of the structure–function, mechanism of action and cellular consequences of this newest member of the G -protein- deamidating dermonecrotic...
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Báo cáo khoa học: Variants of b2-microglobulin cleaved at lysine-58 retain the main conformational features of the native protein but are more conformationally heterogeneous and unstable at physiological temperature potx
... structure are found at the end of strand D and the beginning of strand E, i.e a further loss in D and a stabilization in E of the local b-structure geometry Compared with wt b2m, the cK58-b2m molecule ... [15,18] and requires the checking and redetermination of most of the resonance assignments of the molecule under investigation according to the standard methodology, i.e going through scalar and ... because of the ambiguous assignment of residues from these regions of the molecule Conformational heterogeneity of cK58-b2m and dK58-b2m The whole NMR dataset for cK58-b2m and dK58b2m revealed the...
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Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf
... dependence of the heat capacity of the initial and final conformations (Cp,N2 and Cp,U), the heat capacity of the native state was taken to be a linear function of temperature and that of the unfolded ... between the protein and the buffer, it is reasonable to assume that the heat capacities of both the native and unfolded states depend upon neither the pH nor the protein Ó FEBS 2004 Unfolding and ... of HU proteins from B stearothermophilus [20] and T maritima [18] The upper part of the figure shows the aligned amino acid sequences of each monomer within the homodimers, the ribbon models of...
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Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc
... from the experiments shown in Figs and In each experiment the tissue with the highest expression level was set to 100 The height of the other bars indicates the relative amount of NICN1 mRNA in the ... amino acids The positions of the exon boundaries with respect to the protein sequence are indicated by arrows Expression of the NICN1 gene To investigate the expression profile of the NICN1 gene ... dependent on the metabolic state of the organ As the RNA for the Northern blot and the RT-PCR analysis had been derived from two different anonymous human donors the differences in the observed...
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