DNA Methylation: Basic Mechanisms - Part 5 pot
DNA Methylation: Basic Mechanisms - Part 5 pot
... Pra
d
er-Wi
ll
ian
df
rom Ange
l
man patients. In t
h
is region, non -5
-CG
-3
C-residues were never meth
y
lated. Around exon 1 of the S NRPN
g
ene, more
t
h
an 96% o
f
t
h
e23
5
-
CG
-
3
-
d
inuc
l
eoti
d
es ... R
i
bosomal Gene
s
Th
e5
-
CG
-
3
-rich promoter re
g
ion of the DNA- dependent RNA pol
y
merase
I (rDNA)
g
enes was ana
ly
ze
df
or t
h
e p...
DNA Methylation: Basic Mechanisms - Part 10 pot
... 187,
19
2–1
94
a
ut
i
sm 36
au
xin
75
5-aza-
2
deox
y
c
y
tidine 18
7
5- aza-d
C
18
7
5- azac
y
tidine 73, 19
1
base flippin
g
208–210
b
enzo
[
a
]
pyrene 271
BER-coupled remeth
y
lation 302
b
romo-a
d
jacent
h
omo
l
ogy ... (2001) T
h
eDNAg
l
ycosy
l
as
e
T:G mismatc
h
-speci
fi
ct
h
ymine DNA g
l
ycosy
l
ase represses t
h
yroi
d
transcription
f
actor-1-activate
d
transcription. J Bi...
DNA Methylation: Basic Mechanisms - Part 1 pptx
... or
g
anism, 5- mC a
l
so ma
k
es an appearance (L
yk
oeta
l.
2
000). Even initiall
y
sound skepticism has sometimes to be re-evaluated.
T
h
e
fif
t
h
nuc
l
eoti
d
e, 5- met
h
y
l
-
d
eoxycyti
d
ine ( 5- mC), ... aci
d
o
f
t
h
etu
b
erc
l
e
b
aci
ll
us
.
Su
b
sequent
ly
, 5- mC
h
a
d
a
b
ioc
h
emica
lf
uture as 5-
hyd
rox
y
met
hyl
-C ( 5-
h
m-C) in the DNA of the T-even bacterio...
DNA Methylation: Basic Mechanisms - Part 2 ppsx
... DNMTs Tar
g
eted to Precise DNA Sequences
?
55
4.1 C
h
romatin-Base
d
Targeting 5
6
4.2 Targeting o
f
DNMTS
b
yDNA-Boun
d
TranscriptionFactors 5
9
4.3 T
h
eRNATrigger 6
0
5C
onclusion
s
62
R
e
f
e
r
e
n
ces
... interacting
d
omain;
C
XX
C
,Cys-ric
hd
omain
;
(
E
)
1
2
,
G
l
u
repea
t;
(
GR
)
11
,
G
l
y-Arg repeat;
MBD
,
met
h
y
l
-CpG-
b
in
d
ing
d
omain
;
Hh
H-GPD,DN
A
N-Gl
y
cos...
DNA Methylation: Basic Mechanisms - Part 3 pptx
... ompose
d
o
f small
g
ene-rich re
g
ions intermixed with 5- to 200-kb blocks of repetitiv
e
DNA. The repetitive DNA blocks are usuall
y
meth
y
lated at 5
-
CG
-3
a
n
d5
-
C
NG-3
c
ytosines in ... 2003).
The DNA meth
y
lase inhibitors 5- azaC
y
t and 5- aza-
2
-deox
y
c
y
tidine inhib
-
ite
d
a
d
ventitious s
h
oot in
d
uction in Petun
i
a
l
ea
f
cu
l
tures....
DNA Methylation: Basic Mechanisms - Part 4 ppsx
... J 34 :56 5 57 2
Z
h
ang D, E
h
ri
l
c
h
KC, Supa
k
ar PC, E
h
r
l
ic
h
M (1989) A p
l
ant DNA-
b
in
d
ing protein t
h
at
reco
g
nizes 5- meth
y
lc
y
tosine residues. Mol Cell Biol 9:1 351 –1 356
Zhan
g
Z, ... Fro
g
Virus 3
I
s Completel
y
5
-CG
-
3
-Met
h
y
l
ate
d
1 45
4 Site-Speci
fi
c Promoter M et
h
y
l
ation an
d
Gene Si
l
encin
g
.
1
4
5
4.1 T
h
e E2A Promoter o
f
A
d...
DNA Methylation: Basic Mechanisms - Part 6 pps
... Heteroc
h
romatin
f
ormatio
n
SUMO-1 N-termina
l
Sma
ll
u
b
iquitin-re
l
ate
d
protein
Ubc9 N-terminal SUMO-1 E2 con
j
u
g
atin
g
enz
y
me
PIAS1 N-terminal SUMO-1 E3 li
g
ase
PIA
S
x
α
N-termina
l
SUMO-1 E3
l
igase
Dnmt3L ... ha
d
m
eth
y
lation of the 5
re
g
ion of RA
R
β
.
PML-RAR-induced re
p
ression of RAR
β
t
ranscription was partia
ll
yre
l
ease
db
y 5- aza-
d
C, in
d
icating t
h
a...
DNA Methylation: Basic Mechanisms - Part 7 docx
... Mechanism of DNA- (Cytosine-C5)-MTase
s
The reaction mechanism of c
y
tosine-C5 meth
y
lation was uncovered for th
e
prokar
y
otic DNA- (c
y
tosine-C5)-MTase M.HhaI (Fi
g
. 2; Wu and Santi 19 85;
Wu an
d
Santi ... 1
,
28 759 Bremen, German
y
a.
j
eltsch@iu-bremen.de
1In
t
r
oduct
i
o
n
2
04
2Cata
l
ytic Mec
h
anism o
f
DNA- (Cytosine-C5)-MTases
.
20
6
2.1 Reaction Mec
h
anism o
f
DNA-...
DNA Methylation: Basic Mechanisms - Part 8 pdf
... 24:20
9
–218
E
l-Maarri O (2004) SIRPH anal
y
sis: SNuPE with IP-RP-HPLCfor quantitativemeasure-
ments of DNA meth
y
lation at specific CpG sites. Methods Mol Biol 287:1 95 20
5
El
-Maarri O, Her
b
iniaux ... trans
g
enerational effects. Radiat Res 159 :58 1 59 2
Neuber
g
er MS, Harris RS, Di Noia J, Petersen-Mahrt SK (2003) Immunit
y
throu
g
h
DNA
d
eamination. Tren
d
sBioc
h
em Sci 28:...
DNA Methylation: Basic Mechanisms - Part 9 pps
... specificit
y
of 5- form
y
lc
y
tosine is broad, and includes tar
-
g
eted ( 5- fC
→
G,
5- fC
→
A
,
and 5- f
C
→
T
) and untar
g
eted mutations (Kami
ya
et a
l
. 2002
b
). Deamination o
f
5-
h
y
d
roxymet
h
y
l
cytosine ... is DNA repair.
Th
e p resence o
f
5- met
h
y
l
cytosine may a
ff
ect t
h
e sequence-
d
epen
d
ent repair
of DNA adducts. A 5- meth
y
lc
y
tosine base at t...