ENZYME KINETICS A MODERN APPROACH – PART 1 pps
ENZYME KINETICS A MODERN APPROACH – PART 1 pps
... ENZYMES 12 1
10 .1 The Model / 12 2
10 .1. 1 Interfacial Binding / 12 2
10 .1. 2 Interfacial Catalysis / 12 3
10 .2 Determination of Interfacial Area per Unit
Volume / 12 5
10 .3 Determination of Saturation ... Early Stages of the Reaction / 13 4
11 .2.2 Late Stages of the Reaction / 13 5
11 .3 Relaxation Techniques / 13 5
12 CHARACTERIZATION OF ENZYME STABILITY 14 0
12 ....
ENZYME KINETICS A MODERN APPROACH – PART 3 pps
... model
fitting by floating a single parameter and using a subset of the data that
may be most sensitive to changes in the value of the particular parame-
ter. Subsequently, add parameters and data until it ... process:
[A
t
] = [A
0
] e
−k
1
t
(1. 138)
The value of k
1
can be determined as discussed previously.
From Eqs. (1. 137) and (1. 138) we can deduce that
[B] =
k
1
k
2
[A] =...
ENZYME KINETICS A MODERN APPROACH – PART 7 ppsx
... Guelph, Ontario, Canada N1G 2W1.
15 8
14 4 CHARACTERIZATION OF ENZYME STABILITY
0 20 40 60 80 10 0
10
1
10
2
10
3
10
4
10
5
10
6
D
Time (t)
log
10
[N]
D= 33.3t
Figure 12 .3. Semilogarithmic plot used ... Stability
Decreases in the activity of an enzyme as a function of time, at dif-
ferent temperatures, are shown in Table 12 .2 and Fig. 12 .1 5a. Assuming
that enzym...
ENZYME KINETICS A MODERN APPROACH – PART 10 pps
... 11 9 1 20
plug-flow, 11 6, 11 7, 11 8 1 19
Enzymes, xiii, 4 1 4 3
allosteric, 10 3
cooperative, 10 2 1 15
defined, 41
immobilized, 11 6 1 20
interfacial, 12 1 1 28
kinetic characterization of, 17 4 1 91
multisite, 10 2 1 15
pH and ... 10 3 1 09, 11 3, 11 4 1 15 , 17 8,
17 9 1 81, 18 1 1 84, 18 5
progress curves and, 4 4–4 5, 4 5–4 6,
17 5 1 77, 17 8 1 79
transien...
ENZYME KINETICS A MODERN APPROACH – PART 2 pptx
... hydroxyl ion–catalyzed reaction, k
HA
is the rate constant
for the undissociated acid-catalyzed reaction, and k
A
−
is the rate constant
for the conjugate base–catalyzed reaction.
32 TOOLS AND TECHNIQUES ... k
HA
[HA] + k
A
−
[A
−
] (1. 80)
which can be expressed as
k
c
= k
HA
[HA] + k
A
−
K
a
[HA]
[H
+
]
=
k
HA
+ k
A
−
K
a
[H
+
]
[HA] (1. 81)
Thus, a plot of k
c
versus H...
ENZYME KINETICS A MODERN APPROACH – PART 4 pptx
... (terminal) 7. 6–8 .4 +1 0 1 3
Sulfhydryl (cysteine) 8–9 +6. 5–7 .0
-Amino (lysine) 9. 4 1 0.6 +1 0 1 2
Phenolic hydroxyl (tyrosine) 9. 8 1 0.4 +6.0
Guanidinium (arginine) 11 . 6 1 2.6 +1 2 1 3
can lead to the ... compared, a one-way analysis of variance (ANOVA),
TABLE 4.2 Rate of Hydration of Fumarate to Malate by Fumarase at various
Substrate Concentrations
a
Velocity (a. u.)...
ENZYME KINETICS A MODERN APPROACH – PART 5 pptx
... as
v
V
max
=
[B]
K
+ [B]
(7 .12 )
where
V
max
=
V
max
[A]
K
BA
+ [A]
(7 .13 )
and
K
=
K
A
s
K
AB
+ K
AB
[A]
K
BA
+ [A]
=
K
AB
(K
A
s
+ [A] )
K
BA
+ [A]
(7 .14 )
From determinations of K
and V
max
at ... cooperativity, which can have noninteger values.
Estimates of the parameters k
and n are obtained using standard non-
linear regression procedures available in most m...
ENZYME KINETICS A MODERN APPROACH – PART 6 pptx
... Fig. 11 .4. Equation (11 .11 ) has the general form
y = y
0
+ A · x + B(e
−C·x
− 1) (11 .12 )
12 2 INTERFACIAL ENZYMES
k
on
k
off
Substrate
Droplet
Interface
Figure 10 .1. Binding of an interfacial enzyme ... Ax
becomes greater than that of the exponential term, and the shape of the
curve approaches that of a straight line. Valuable information can be
gained from analysis of the ear...
ENZYME KINETICS A MODERN APPROACH – PART 8 ppt
... Reboud-Raveax, J P. Mazalelyrat, and
M. Wakselman, Biochim. Biophys. Acta 10 76, 40 1 4 05 (19 91) .
WERE INITIAL VELOCITIES MEASURED? 17 5
Since significant meaning is placed on these measured constants
and ... k
cat
(s
1
)
k
cat
/K
m
(s
1
M
1
)
1 0.5 0–2 .5 6 0.897 296
2 1. 0–6 .0 8 0 .18 4 36.0
3 0.5 0–8 .0 6 2.97 18 30
4 0.5 0–2 .5 7 0.407 15 2
5 2. 5 1 2.0 10 0 .18 3...
ENZYME KINETICS A MODERN APPROACH – PART 9 docx
... Peptide
Substrate Lysine–Proline–Alanine– Glutamic Acid– Phenylalanine–Phenylalanine
(NO
2
)–Alanine–Leucine
a
First-Order Rate Constant (min
1
) ± SD
pH 1. 1 2.0 3.0
Wild-type 1. 10 ± 0 .10 0. 519 ±0.058 0 .10 9 ... lysine–proline–alanine–glutamic acid–phenylalanine–
phenylalanine (NO
2
)–alanine–leucine.
c
ss2, substrate consisting of the peptide leucine–serine–phenylalanine (NO
2
)–n...
Từ khóa:
- summary artificial intelligence a modern approach
- literacy for the 21st century a balanced approach chapter 1
- bài giảng ứng dụng tin học trong xây dựng part 1 ppsx
- bài tập tin học đại cương part 1 ppsx
- giáo trình công nghệ chế biến thủy hải sản part 1 ppsx
- handling a database exception part 1 raiserror