ENZYME KINETICS A MODERN APPROACH – PART 1 pps

... ENZYMES 12 1 10 .1 The Model / 12 2 10 .1. 1 Interfacial Binding / 12 2 10 .1. 2 Interfacial Catalysis / 12 3 10 .2 Determination of Interfacial Area per Unit Volume / 12 5 10 .3 Determination of Saturation ... Early Stages of the Reaction / 13 4 11 .2.2 Late Stages of the Reaction / 13 5 11 .3 Relaxation Techniques / 13 5 12 CHARACTERIZATION OF ENZYME STABILITY 14 0 12 ....

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... model fitting by floating a single parameter and using a subset of the data that may be most sensitive to changes in the value of the particular parame- ter. Subsequently, add parameters and data until it ... process: [A t ] = [A 0 ] e −k 1 t (1. 138) The value of k 1 can be determined as discussed previously. From Eqs. (1. 137) and (1. 138) we can deduce that [B] = k 1 k 2 [A] =...

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... Guelph, Ontario, Canada N1G 2W1. 15 8 14 4 CHARACTERIZATION OF ENZYME STABILITY 0 20 40 60 80 10 0 10 1 10 2 10 3 10 4 10 5 10 6 D Time (t) log 10 [N] D= 33.3t Figure 12 .3. Semilogarithmic plot used ... Stability Decreases in the activity of an enzyme as a function of time, at dif- ferent temperatures, are shown in Table 12 .2 and Fig. 12 .1 5a. Assuming that enzym...

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... 11 9 1 20 plug-flow, 11 6, 11 7, 11 8 1 19 Enzymes, xiii, 4 1 4 3 allosteric, 10 3 cooperative, 10 2 1 15 defined, 41 immobilized, 11 6 1 20 interfacial, 12 1 1 28 kinetic characterization of, 17 4 1 91 multisite, 10 2 1 15 pH and ... 10 3 1 09, 11 3, 11 4 1 15 , 17 8, 17 9 1 81, 18 1 1 84, 18 5 progress curves and, 4 4–4 5, 4 5–4 6, 17 5 1 77, 17 8 1 79 transien...

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... hydroxyl ion–catalyzed reaction, k HA is the rate constant for the undissociated acid-catalyzed reaction, and k A − is the rate constant for the conjugate base–catalyzed reaction. 32 TOOLS AND TECHNIQUES ... k HA [HA] + k A − [A − ] (1. 80) which can be expressed as k c = k HA [HA] + k A − K a [HA] [H + ] =  k HA + k A − K a [H + ]  [HA] (1. 81) Thus, a plot of k c versus H...

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... (terminal) 7. 6–8 .4 +1 0 1 3 Sulfhydryl (cysteine) 8–9 +6. 5–7 .0 -Amino (lysine) 9. 4 1 0.6 +1 0 1 2 Phenolic hydroxyl (tyrosine) 9. 8 1 0.4 +6.0 Guanidinium (arginine) 11 . 6 1 2.6 +1 2 1 3 can lead to the ... compared, a one-way analysis of variance (ANOVA), TABLE 4.2 Rate of Hydration of Fumarate to Malate by Fumarase at various Substrate Concentrations a Velocity (a. u.)...

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... as v V  max = [B] K  + [B] (7 .12 ) where V  max = V max [A] K BA + [A] (7 .13 ) and K  = K A s K AB + K AB [A] K BA + [A] = K AB (K A s + [A] ) K BA + [A] (7 .14 ) From determinations of K  and V  max at ... cooperativity, which can have noninteger values. Estimates of the parameters k  and n are obtained using standard non- linear regression procedures available in most m...

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... Fig. 11 .4. Equation (11 .11 ) has the general form y = y 0 + A · x + B(e −C·x − 1) (11 .12 ) 12 2 INTERFACIAL ENZYMES k on k off Substrate Droplet Interface Figure 10 .1. Binding of an interfacial enzyme ... Ax becomes greater than that of the exponential term, and the shape of the curve approaches that of a straight line. Valuable information can be gained from analysis of the ear...

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... Reboud-Raveax, J P. Mazalelyrat, and M. Wakselman, Biochim. Biophys. Acta 10 76, 40 1 4 05 (19 91) . WERE INITIAL VELOCITIES MEASURED? 17 5 Since significant meaning is placed on these measured constants and ... k cat (s 1 ) k cat /K m (s 1 M 1 ) 1 0.5 0–2 .5 6 0.897 296 2 1. 0–6 .0 8 0 .18 4 36.0 3 0.5 0–8 .0 6 2.97 18 30 4 0.5 0–2 .5 7 0.407 15 2 5 2. 5 1 2.0 10 0 .18 3...

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... Peptide Substrate Lysine–Proline–Alanine– Glutamic Acid– Phenylalanine–Phenylalanine (NO 2 )–Alanine–Leucine a First-Order Rate Constant (min 1 ) ± SD pH 1. 1 2.0 3.0 Wild-type 1. 10 ± 0 .10 0. 519 ±0.058 0 .10 9 ... lysine–proline–alanine–glutamic acid–phenylalanine– phenylalanine (NO 2 )–alanine–leucine. c ss2, substrate consisting of the peptide leucine–serine–phenylalanine (NO 2 )–n...

Ngày tải lên: 13/08/2014, 07:20