... C-terminal arginine,was designed as follows (Fig. 3A) . Two overlappingoligonucleotide pairs 5¢-GAGGTCGACATGCGCTGCAAGTCGTCGAAGGAGTGCCTGGTCAAGTGCAAGCAG-3¢,3¢-CTCCAGCTGTACGCGACGTTCAGCAGCTTCCTCACGGACCAGTTCACGTTCGTCCGCTGCCCGGCC-5¢ ,and5 ¢-GCGACGGGCCGGCCGAACGGCAAGTGCATGAACCGGAAGTGCAAGTGCTACCCGTGAG-3¢,3¢-GGCTTGCCGTTCACGTACTTGGCCTTCACGTTCACGATGGGCACTCCTAG-5¢,respect-ively, ... 5¢-GAGGTCGACATGCGCTGCAAGTCGTCGAAGGAGTGCCTGGTCAAGTGCAAGCAG-3¢,3¢-CTCCAGCTGTACGCGACGTTCAGCAGCTTCCTCACGGACCAGTTCACGTTCGTCCGCTGCCCGGCC-5¢ ,and5 ¢-GCGACGGGCCGGCCGAACGGCAAGTGCATGAACCGGAAGTGCAAGTGCTACCCGTGAG-3¢,3¢-GGCTTGCCGTTCACGTACTTGGCCTTCACGTTCACGATGGGCACTCCTAG-5¢,respect-ively, ranging in ... linear gradient,starting after 6 min and ranging from 0% to 30% up to100 min with a flow rate of 200 lLÆmin)1(Fig. 3 D), wasapplied and the toxin was collected, dried (Speed VacÒPlus) and...