Báo cáo khoa học: "Endocrine Profiles, Haematology and Pregnancy Outcomes of Late Pregnant Holstein Dairy Heifers Sired by Bulls Giving a High or Low Incidence of Stillbirth" ppt
... Gustafsson H, Kindahl H: En- docrine profiles, haematology and pregnancy outcomes of late pregnant Holstein dairy heifers sired by bulls giving a high or low incidence of stillbirth. Acta vet. scand. ... occurrence of stillbirth or poor calf viability and endocrine patterns, haematology and some other related parameters such as placental char...
Ngày tải lên: 12/08/2014, 15:20
... (NlaIII site), releasing 21–22 bp tags starting with the NlaIII recognition sequence. A second adaptor (GEX adaptor 2) was ligated at the site of MmeI cleavage, and the adapter-ligated cDNA tags ... using a sequencing -by- synthesis method in accordance with the manufacturer’s instructions. Tag-to-gene assignment and functional categorization Sequencing quality evaluation and data...
Ngày tải lên: 22/03/2014, 17:20
... points of the proteins (52.32 °C for the wild-type and ‘not detectable’ for W14 0A and W140O). Flanagan et al. [15] reported that multiple mutations can cause large changes in the average conformation ... Salmon testes DNA and some analytical grade chemicals such as EDTA, Tris ⁄ HCl, CaCl 2 , NaCl and mineral oil were obtained from Sigma (St Louis, MO, USA). Salmon testes DNA for t...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo khoa học: "What lies beneath: Semantic and syntactic analysis of manually reconstructed spontaneous speech" pdf
... the annotated SSR data) can be given a semantic analysis in the following sec- tions. For each well-formed and grammatical sen- tence, all (non-auxiliary and non-modal) verbs were identified by annotators ... Proceed- ings of the Human Language Technology Confer- ence/North American chapter of the Association of Computational Linguistics (HLT/NAACL), Boston, MA. Sameer Pradhan, J...
Ngày tải lên: 20/02/2014, 07:20
Tài liệu Báo cáo khoa học: "Prosodic Aids to Syntactic and Semantic Analysis of Spoken English" ppt
... Rowles and Xiuming Huang AI Systems Section Australia and Overseas Telecommunications Corporation Telecommunications Research Laboratories PO Box 249, Clayton, Victoria, 3168, Australia Internet: ... morphemes and pauses) and rhythm. While all of these are important cues, we are currently focussing on pitch and pauses as these are easily extracted from the waveform and o...
Ngày tải lên: 20/02/2014, 21:20
Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot
... Koshiyama A, Hamada FN, Namekawa SH, Iwabata K, Sugawara H, Sakamoto A, Ishizaki T & Sakaguchi K (2006) Sumoylation of a meiosis-specific RecA homo- log, Lim15/Dmc1, via interaction with the small ... software (GE Healthcare Bio-Sciences). Acknowledgements We thank Montserrat Anguera, Jennifer Erwin and Janice Ahn for critical reading of the manuscript, and all members of Sak...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt
... membrane and periplasmic space, whereas PilM, PilN and PilO are inner membrane proteins that probably form part of the assembly platform and are involved in the assembly of the DNA translocator complex ... CCA pilQ 5¢⁄EcoRI CAC CTT CCC C GA ATT CCT CGC C 832 3¢⁄HindIII CAC CCT AAG CTT CAC CTA GGG CAC pilA4 5¢⁄EcoRI GAC GCG GTG GAA TTC CAG GAG 291 3¢⁄SalI CAA CGC TAG C GT CGA CGC CAT...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
... (DH cal ) was calculated by the integration of the curve covering area (T m was taken as the curve peak point) using origin soft- ware. Acknowledgements This work wass partially supported by a grant ... measured between 300 and 550 nm with a scanning speed of 150 nmÆs )1 and an excitation slit of 5.0 nm. Calorimetric measurements Thermal analysis of protein denaturation wa...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells pot
... the attached cells according to Bligh and Dyer [20]. After lipid extraction the aqueous and organic phases were separated and the radioactivity of each phase was measured. Incorporation was determined ... iodine vapour and ultraviolet light, and assigned by means of standards. Radiometric measurements of lipid spots were made by liquid scintillation using a Beckman 6000-...
Ngày tải lên: 17/03/2014, 11:20
Báo cáo khoa học: Refined solution structure and backbone dynamics of the archaeal MC1 protein ppt
... the 15 N-NMR relaxation data, by using the model-free approach suggested by Lipari and Szabo [21,22] and extended by Clore et al. [23]. This approach is applicable for the case of statistically independent ... acceptor (A) was smaller than 3.5 A ˚ and the D–H A angle was larger than 135°. Acknowledgements The authors would like to thank S. Goffinont (CBM, Orle ´ ans, France) f...
Ngày tải lên: 22/03/2014, 17:20