... binding and RNA synthesis from the HCV minus-strand RNA. Altogether these results suggest that several domains of the RNA fragment comprising the 341 nt from the 3Â- end of the minus-strand RNA ... 3883 Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3Â-end of...
Ngày tải lên: 20/02/2014, 01:20
... gene assay in in vivo results. Experimental procedures Parasites The NMRI strain of S. mansoni was maintained in snails (Biomphalaria glabrata) and Syrian golden hamsters (Mesocricetus auratus ). ... Sequence alignment. (A) Alignment of DNA binding domain (C domain) and its C-terminal extension. (B) Alignment of ligand binding domain (E domain) (after helix 2). Helices as de...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx
... 1046–1055 ª 2006 The Authors Journal compilation ª 2006 FEBS 1049 Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase Nadthanan ... Among them, spheru- Fig. 2. Structural alignment of amino acid sequence by motifs, of spherulin 3b with spherulin 3a and other mem...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha pptx
... averages of three measurements. Ó FEBS 2004 Two FMN groups in NAD + -reducing [NiFe]-hydrogenase (Eur. J. Biochem. 271) 805 Selective release and function of one of the two FMN groups in the cytoplasmic ... demonstrate that the release or re-binding of flavin at the FMN- a binding site occurs only in reduced enzyme and that FMN...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... Consequently, the molecular studies of the interaction of SAgs with their s pecifics ligands will not only advance understanding of the physiological mechanisms of these molecules, but may lead to the development ... Fig. 1B–D, expression of the mutant yields only monomeric SSA, free of dimer. T-cell proliferation assay We next analyzed the ability of recomb...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: "Case markers and Morphology: Addressing the crux of the fluency problem in English-Hindi SMT" pot
... create Hindi language models using the target side of the train- ing corpus. Training, tuning, and decoding were performed using the Moses toolkit 3 . Tuning (learning the λ values discussed in section ... 4th IJCNLP of the AFNLP, pages 800–808, Suntec, Singapore, 2-7 August 2009. c 2009 ACL and AFNLP Case markers and Morphology: Addressing the crux of the...
Ngày tải lên: 08/03/2014, 00:20
Báo cáo khoa học: A new and efficient method for inhibition of RNA viruses by DNA interference pptx
... siDNA was obtained by hybridization of sense strand (5Â-GTTGCTCTGGAAAACTCATTT-3Â) and anti- sense strand (5Â-ATGAGTTTTCCAGAGCAACTT-3Â), and siRNA was synthesized using DNA templates (sense strand, 5Â-AAATGAGTTTTCCAGAGCAACCCTGTCTC-3Â; ... siDNAs than of siRNAs is necessitated by the lower stability of RNA DNA than of RNA RNA duplexes formed within RISC. On the other hand,...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species pptx
... 2008 FEBS REVIEW ARTICLE Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species Hideki Sumimoto Medical Institute of Bioregulation, Kyushu University, ... reductase (FRO) of land plants [38]. To acquire iron from soils of low iron availability, land plants such as Arabidopsis thaliana H. Sumimoto Structure, reg...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx
... ricin, in particular its route into target cells and the fate of its two subunits, RTA and the cell-binding galactose-specific lectin ricin toxin B chain (RTB), are essential to gain further insights ... were CP172 5Â-ATATTCCCCAAACAATACCC-3Â and the anti- sense primer CP133 5Â-TTAAAACTGTGACGATGGT GGA-3Â with the TAA termination anticodon shown in bold. Amplification reactions w...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Salicylic acid and the hypersensitive response initiate distinct signal transduction pathways in tobacco that converge on the as-1-like element of the PR-1a promoter pot
... (2000) Tobacco transcription factor TGA2.2 is the main component of as-1- binding factor ASF-1 and is involved in salicylic acid- and auxin- inducible expression of as-1-containing target promoters. ... related elements can be functional in the context of the PR-1a promoter, suggesting that the same or similar factors are involved in transcription via as-1 a...
Ngày tải lên: 23/03/2014, 15:21
Báo cáo khoa học: Membrane compartments and purinergic signalling: the role of plasma membrane microdomains in the modulation of P2XR-mediated signalling pot
... to the fine-tuning of P2XR-mediated signalling by controlling the local kinetics of extracellular agonist degradation and the integration with different purinergic signal inputs (via other purinoceptors ... to specific signalling pathways. Given that both P2X and other purinergic receptors (i.e. P1 and P2Y) can localize differentially in microdo- mains of the pl...
Ngày tải lên: 30/03/2014, 02:20
Báo cáo khoa học: "Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis" ppsx
... Localization and level of SOD1 in developing mouse embryos 237 Table 1. Comparison of cytoplasmic Cu/Zn superoxide dismutase expression in developing embryonic organs following embryonic da y Organs ... 233 240 *Corresponding author Tel: +82-43-261-2596; Fax: +82-43-271-3246 E-mail: synam@cbu.ac.kr Immunohistochemical identification and quantitative analysis o...
Ngày tải lên: 07/08/2014, 20:23
Báo cáo khoa học: " Nucleotide identity and variability among different Pakistani hepatitis C virus isolates" potx
... genotype 1 and 4 and 1 and 3 respectively. Background Hepatitis C virus (HCV) belongs to the family Flaviviridae, genus Hepacivirus and is responsible for the second most common cause of viral hepatitis ... not for citation purposes) Virology Journal Open Access Research Nucleotide identity and variability among different Pakistani hepatitis C virus isolate...
Ngày tải lên: 12/08/2014, 04:20
Báo cáo khoa học: "Genetic diversity and phylogeography of Seewis virus in the Eurasian common shrew in Finland and Hungary" ppsx
... opportunity to investigate the genetic diversity and phylogeography of SWSV. Archival liver tissues from 88 Eurasian common shrews, trapped in Finland in 1982 and in Hungary during 1997, 1999 and 2000 ... of Eurasian common shrews are present in Finland and Hungary. Whether or not the sub-lineages of SWSV can be traced to potentially distinct evolutio...
Ngày tải lên: 12/08/2014, 04:21
báo cáo khoa học: " Nucleotide diversity and linkage disequilibrium in 11 expressed resistance candidate genes in Lolium perenne" ppt
... article Nucleotide diversity and linkage disequilibrium in 11 expressed resistance candidate genes in Lolium perenne Yongzhong Xing 1,2 , Uschi Frei 1 , Britt Schejbel 1 , Torben Asp 1 and Thomas ... tracing in GRASP within about 1 kb fragments of expressed resist- ance candidate genes, (2) compare the nucleotide diver- sity within and between differen...
Ngày tải lên: 12/08/2014, 05:20