Báo cáo khoa học: "Antiviral activity of gliotoxin, gentian violet and brilliant green against Nipah and Hendra virus in vitro" ppsx

... gliotoxin against numer- ous viruses including poliovirus types 1, 2 and 3, rhinovi- rus strain HGP, ECHO virus types 12 and 28, measles virus, coxsackie virus, Sendai virus, influenza virus and Newcastle ... structures of a. brilliant green, b. gentian violet and c. gliotoxin. Table 1: IC 50 , CC 50 and therapeutic index (TI) values calculated for each compound a...

Ngày tải lên: 12/08/2014, 04:20

... mechanisms involved in PrP Sc propagation, aggregation and neuroinvasion in vivo [22]. In this study, possible in uences of pyridine nucleotides on the propagation of PrP Sc from scrapie- infected ... the proteinase K (PK)-resistant isoform in the presence of scrapie PrP Sc in PMCA. Results The propagating activities of PrP Sc in PMCA are remarkably enhanced in the pr...

Ngày tải lên: 07/03/2014, 03:20

... examined cal- pain 7–IST1 interactions by in vitro and in vivo binding experiments, using purified recombinant proteins and cultured mammalian cells expressing epitope-tagged proteins. We also investigated ... whether MIM1 and MIM2 bind to only one MIT domain or bind to each of the two MIT domains of calpain 7, and it is not known why interaction between calpain 7 and the MIM...

Ngày tải lên: 18/02/2014, 04:20

... conformational transition upon binding to BchH may bring the integrin I domain of BchD into contact with the integrin-binding motif of BchH, simultaneously triggering porphyrin metallation. This would ... one type of molecular chaperones and their function is to control the fate of proteins or DNA. This is done by facilitating protein folding and unfolding, assembly and disassem...

Ngày tải lên: 19/02/2014, 12:20

... features of the catalytic site (i.e. a catalytic triad of Ser-His-Glu, an oxyanion hole, a p-cation-binding site, and an acyl-binding pocket) are the same in AChE and BuChE (Fig. 1). The acyl-binding pocket, ... excess-substrate binding appears to involve the intricate interplay of a variety of residues in the active site. pH dependence of A328W The A328W mutant has a remark...

Ngày tải lên: 20/02/2014, 23:20

... environment and are essentially an indication of substrate specificity. Full understanding of sHsp substrate interactions will require not only considera- tion of substrate binding sites and binding interactions, ... deter- mined by the N-terminal arm. The N-terminus of PsHsp18.1.C-I was also implicated in substrate interactions in bis-ANS binding experiments [15]. As it is pro...

Ngày tải lên: 07/03/2014, 15:20

... from the N-terminus domain of histone H2A: buforin I (39 amino acids), parasin I (21 amino acids), and hipposin (51 amino acids ) from the Asian toad stomach and catfish and halibut skin mucus, respect- ively, ... example, bovine lactoferricin is derived from lactoferrin, an iron bin ding protein, by pepsin cleavage of its N-terminus [9]. In crustaceans, antimicrobial peptides have...

Ngày tải lên: 07/03/2014, 16:20

... supernatant. Fig. 6. Autophosphorylation of recombinant Ndk and mutant proteins. (A) Ndk and mutant proteins (1 lg) were incubated in the presence of 10 lCi of [c- 32 P]ATP in 20 lL of reaction volume. The reaction ... H53Q resulted in the loss of both autophosphorylation and nucleotide binding activity (Figs 3, 6 and 7). Thus only His117 is critical for autophosphor...

Ngày tải lên: 08/03/2014, 08:20

... (A) Native gels showing in situ enzymatic staining for ATP or ADP hydrolysis activity of the Q-fraction of NtrC1 C . Arrows indi- cate positions of the NtrC1 C and apyrase proteins located by Coo- massie ... chromatography permitting quantification of fluxes in the concen- trations of ATP, ADP and AMP, beginning with an initial concentration of 10 mm ATP (Fig. 1B). The app...

Ngày tải lên: 16/03/2014, 04:20

... nature of CH 2 H 4 folate inhibition of the oxidase activity. To examine the relation between CH 2 H 4 folate and O 2 , and to ascer- tain the binding constant of CH 2 H 4 folate to the reduced and ... attempts to gain structural information of FDTS complexes with folates and nicotinamides. The Scheme 3. The proposed binding and release kinetic mechanism of FDTS (see text...

Ngày tải lên: 23/03/2014, 04:21