báo cáo khoa học: " Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida" pot

... 10:4 http://www.biomedcentral.com/1471-2229/10/4 Page 4 of 11 RESEARC H ARTIC LE Open Access Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida Izaskun Mallona 1 , Sandra Lischewski 2 , ... log-transformed and used as input for the NormFinder tool, which fitted this data into a Figure 3 Express...

Ngày tải lên: 12/08/2014, 03:21

... NF n+1 ) and indicates the minimum number of reference genes necessary for an Ct distribution of each candidate reference gene among the 22 samplesFigure 2 Ct distribution of each candidate reference ... apomictic and sexual reproduc- tion within Brachiaria makes it an interesting system for understanding the molecular pathways involved in both modes of reproduction....

Ngày tải lên: 12/08/2014, 03:20

... basis of ligand recognition by PABC, a highly specific peptide- binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. EMBO J 23, 272–281. 9 Craig A (1998) Interaction of polyadenylate-binding protein ... cis-element is involved in regulating PABP1 mRNA translation during growth stimulation [14]. A number of mRNAs coding for fac- tors involved in mRNA tr...

Ngày tải lên: 18/02/2014, 12:20

... ed-end actin and determine the level and distr ibution o f F -actin [34]. Therefore, it i s tempting to speculate that interactions between monomeric G-actin and actin-binding proteins are a potential ... fixed, permeabilized and stained for F-actin with rhodamine-conju- gated phalloidin. Th e effects of toxin B and latrunculin B on the actin filaments was examined by preincubating...

Ngày tải lên: 19/02/2014, 16:20

... miRNAs in noncoding transcripts, and intronic miRNAs in protein-coding transcripts [8,9]. Based on the accumulating knowledge on miRNA biogenesis, we report here the development of vectors in which ... lgÆmL )1 streptomycin. For transfections, cells were grown in 6-well or 12-well plates and then switched into Opti-MEM I media before being transfected with 1 lgor 0.5 lg of...

Ngày tải lên: 07/03/2014, 11:20

... substrate and the inherent partioning of quinol into the Q o site. The Fig. 3. Steady-state level of the iron–sulfur protein (ISP) in G167E. Immunoblots of denaturing gels, loaded with 40 lg of protein ... the protein–protein interaction between the n-sulfur protein hinge region and the cytochrome b extramembranous cd2 helix is important for maintaining the structure of the...

Ngày tải lên: 07/03/2014, 15:20

... 2743 Formation of nucleoprotein RecA filament on single-stranded DNA Analysis by stepwise increase in ligand complexity Irina P. Bugreeva, Dmitry V. Bugreev and Georgy A. Nevinsky Institute of Chemical ... I 50 values for interactions of different ligands with the high-affinity DNA-binding center of E. coli RecA filament. Ligand (inhibition of d(pT) 20 ) I 50 , M* –lgI 50 Ligand...

Ngày tải lên: 30/03/2014, 16:20

... complementarity Before considering combining and validating the outputs of individual techniques, it is informative to look at some notion of “complementarity” be- tween techniques, in terms of how many ... the techniques in our study produce more candidates that are not in the gold standard: this will be an important fact to keep in mind when tackling the task of combining...

Ngày tải lên: 31/03/2014, 21:20

...

Ngày tải lên: 07/08/2014, 18:21

... prognostic ability of bidimensional measurement of primary tumor and ret- ropharyngeal nodes using MRI findings. After analyzing trade-off, we chose 15 cm 2 as the cut-off point in the validation cohort ... an as means of deter- mining the stage of tumors, including NPC, before they are treated [13]. MRI is superior to CT scan for diag- nosing the gross extent of tumor infil...

Ngày tải lên: 09/08/2014, 09:20